Immunohistochemical Demonstration of S-100 Protein in Epithelial Cells of Bovine Oviduct

Kamiya, Shinji; Tajika, T; Daigo, M.

doi:10.1159/000146801

Cited by 2 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They also suggested that S-100 varies among different species rather than among different tissues of a given species. Although the presence of S-100 in the bovine Fallopian tube was shown by Kamiya et al (1989), the subunit distribution and the heterogeneity of the epithelial cell population observed in the present study was not examined in detail. In agreement with the results of Kamiya et al (1989), we found that the cytoplasm of non-ciliated cells of the ampulla and of fimbriae was diffusely positive for S-100a(a~) and S-100~ during the whole oestrous cycle.…”

Section: Discussionmentioning

confidence: 68%

“…The different labelling results with anti-S-100a(R~) or [3 obtained by Kamiya et al (1989) in non-ciliated cells in the isthmus may be explained by the use of different antibodies (different source) or by differences in sampling: as the isthmus epithelial lining merges into the S-100-negative uterus epithelium gradually, the site of sample collection could be crucial.…”

Section: Discussionmentioning

confidence: 99%

“…Only a single report has dealt with the distribution of S-100 in bovine oviduct epithelium during the oestrus cycle by means of immunohistochem-istry (Kamiya et al, 1989), but subunit composition was not considered. These authors found reactivity for S-100 in all types of epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

S-100 protein subunits in bovine oviduct epithelium:In situ distribution and changes during primary cell culture

Walter

Miller

1996

Histochem J

View full text Add to dashboard Cite

Cultures of bovine oviduct epithelial cells are widely used in co-culture systems to improve the results of in vitro fertilization. The aim of the present study was to evaluate the suitability of S-100 protein as a differentiation marker for bovine oviduct epithelial cells in vitro. The distribution of S-100 alpha and S-100 beta was examined immunohistochemically in bovine oviduct epithelium in situ and in primary cell cultures derived from it. Three segments of the Fallopian tube (isthmus, ampulla and fimbriae) were compared and analysed during different stages of the oestrus cycle (luteal phase and follicular phase). Ciliated and non-ciliated cells of the epithelium reacted with anti-S-100 alpha, S-100a (alpha beta) and S-100 beta antibodies, except for isthmic non-ciliated cells, which did not bind anti-S-100 beta or anti-S-100a (alpha beta). In addition, basal cells never showed immunoreactivity for S-100. In confluent monolayers of cultured oviduct epithelial cells, disappearance of reactivity for S-100 paralleled morphological signs of dedifferentiation (loss of cilia, cytoplasmic vacuolization). Free-floating oviduct epithelial cells, in contrast, retained morphological differentiation and still expressed S-100 antigen even after seven days in vitro. The immunohistochemical findings were confirmed by polyacrylamide gel electrophoresis and Western blotting. The results indicate that the presence of S-100 is closely connected to morphological differentiation and to the specific functional condition of bovine oviduct epithelial cells.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

S-100 protein subunits in bovine oviduct epithelium:In situ distribution and changes during primary cell culture

Walter

Miller

1996

Histochem J

View full text Add to dashboard Cite

show abstract

“…In the oviduct, these proteins were linked with epithelial cells (Kamiya et al. ). Acetylated α ‐tubulin is present in various microtubule structures.…”

Section: Introductionmentioning

confidence: 99%

“…S100 proteins have been implicated in a variety of intracellular and extracellular functions (Heizmann et al 2002;Donato 2003). In the oviduct, these proteins were linked with epithelial cells (Kamiya et al 1989). Acetylated a-tubulin is present in various microtubule structures.…”

Section: Introductionmentioning

confidence: 99%

An Immunohistochemical Observations on the Oviduct of the Goat

Marettová

Maretta

2014

Reprod Domestic Animals

View full text Add to dashboard Cite

The oviduct has an important role in regulating transport of gametes and fertilization. The main role in these functions has a smooth muscle cells and ciliated epithelium lining the oviduct. All functions are under the influence of hormonal and nervous system. The objective of this study was immunohistochemically to examine the following structures: lining epithelium, smooth muscle cells, elastic fibres and nerve fibres. For this purpose, the following antibodies were used: cytokeratin 18, S-100 protein, acetylated α-tubulin, smooth muscle actin, desmin and elastin. Ciliary and secretory cells of the lining epithelium were positive for cytokeratin 18 and S-100 protein. Cilia and the basal body-associated structures of ciliary cells were positive to acetylated α-tubulin. Smooth muscle cells (SMC) in mucosa and of the muscular layer were positive for α-smooth muscle actin (SMA) and desmin. High density of nerve fibres positively reacted to acetylated α-tubulin and S100 protein was present in the mucosa, muscular layer and serosa. Elastic fibres positive for elastin form a dense network at the base of the mucosal folds and in the muscle layer. A dense network of these fibres is accompanying the blood vessels. It is supposed that together with smooth muscle cells they are involved in the transport of ovum and in blood flow regulation.

show abstract

Immunohistochemical Demonstration of S-100 Protein in Epithelial Cells of Bovine Oviduct

Cited by 2 publications

References 6 publications

S-100 protein subunits in bovine oviduct epithelium:In situ distribution and changes during primary cell culture

S-100 protein subunits in bovine oviduct epithelium:In situ distribution and changes during primary cell culture

An Immunohistochemical Observations on the Oviduct of the Goat

Contact Info

Product

Resources

About