Immunohistochemical demonstration of salmon olfactory glutathione S-transferase class pi (N24) in the olfactory system of lacustrine sockeye salmon during ontogenesis and cell proliferation

Yanagi, S.; Kudo, Hideaki; Doi, Yoshiaki; Yamauchi, Kazuyo; Ueda, Hiroshi

doi:10.1007/s00429-004-0392-3

Cited by 21 publications

(14 citation statements)

References 24 publications

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“…This peculiar cell type is a true sensory neuron that sends its axon to well-defined areas in the olfactory bulb . Subpopulations of OSNs with xenobiotic transformation capabilities were characterized (Starcevic et al, 1993;Starcevic & Zielinski, 1995;Yanagi et al, 2004), and further details with respect to the G-protein-coupled receptor proteins and the transduction pathways added to the understanding of the function of the fish olfactory system (Belanger et al, 2003;Cao et al, 1998;Hansen et al, 2003Pfister & Rodriguez, 2005;Speca et al, 1999). It is now clear that several subsets of distinct morphological forms of teleost OSNs exist, based on odorant ligand, receptor protein, G-protein subunit, and second messenger activation (e.g., Hansen et al, 2003Luu et al, 2004;Sato & Suzuki, 2001).…”

Section: Introductionmentioning

confidence: 99%

Diversity in the olfactory epithelium of bony fishes: Development, lamellar arrangement, sensory neuron cell types and transduction components

2005

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In this study we use a taxon-based approach to examine previous, as well as new findings on several topics pertaining to the peripheral olfactory components in teleost fishes. These topics comprise (1) the gross anatomy of the peripheral olfactory organ, including olfactory sensory neuron subtypes and their functional parameters, (2) the ultrastructure of the olfactory epithelium, and (3) recent findings regarding the development of the nasal cavity and the olfactory epithelium. The teleosts are living ray-finned fish, and include descendants of early-diverging orders (e.g., salmon), specialized descendants (e.g., goldfish and zebrafish), as well as the Acanthopterygii, numerous species with sharp bony rays, including perch, stickleback, bass and tuna. Our survey reveals that the olfactory epithelium lines a multi-lamellar olfactory rosette in many teleosts. In Acanthopterygii, there are also examples of flat, single, double or triple folded olfactory epithelia. Diverse species ventilate the olfactory chamber with a single accessory nasal sac, whereas the presence of two sacs is confined to species within the Acanthopterygii. Recent studies in salmonids and cyprinids have shown that both ciliated olfactory sensory neurons (OSNs) and microvillous OSNs respond to amino acid odorants. Bile acids stimulate ciliated OSNs, and nucleotides activate microvillous OSNs. G-protein coupled odorant receptor molecules (OR-, V1R-, and V2R-types) have been identified in several teleost species. Ciliated OSNs express the G-protein subunit G(alphaolf/s), which activates cyclic AMP during transduction. Localization of G protein subunits G(alpha0) and G(alphaq/11) to microvillous or crypt OSNs, varies among different species. All teleost species appear to have microvillous and ciliated OSNs. The recently discovered crypt OSN is likewise found broadly. There is surprising diversity during ontogeny. In some species, OSNs and supporting cells derive from placodal cells; in others, supporting cells develop from epithelial (skin) cells. In some, epithelial cells covering the developing olfactory epithelium degenerate, in others, these retract. Likewise, there are different mechanisms for nostril formation. We conclude that there is considerable diversity in gross anatomy and development of the peripheral olfactory organ in teleosts, yet conservation of olfactory sensory neuron morphology. There is not sufficient information to draw conclusions regarding the diversity of teleost olfactory receptors or transduction cascades.

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Section: Introductionmentioning

confidence: 99%

Diversity in the olfactory epithelium of bony fishes: Development, lamellar arrangement, sensory neuron cell types and transduction components

2005

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“…In teleosts, Nile tilapia (Oreochromis niloticus) ORNs expressed an ubiquitin C-terminal hydrolase (EC 3.4.19.12) homologous to PGP9.5 (Mochida et al, 2002). An immunohistochemical study of GST during ontogeny indicated that GST started to be synthesized in embryonic ORNs of lacustrine sockeye salmon after the projection of olfactory nerve axons to the olfactory bulb (Yanagi et al, 2004). These findings strongly suggest that salmon OMP is expressed as well as mammalian OMP in mature ORNs, and GST is expressed following the appearance of PGP9.5 in semi-mature ORNs.…”

Section: Discussionmentioning

confidence: 66%

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Kudo

Doi

Ueda

et al. 2009

Comparative Biochemistry and Physiology Part A: Molecular &

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Despite the importance of olfactory receptor neurons (ORNs) for homing migration, the expression of olfactory marker protein (OMP) is not well understood in ORNs of Pacific salmon (Genus Oncorhynchus). In this study, salmon OMP was characterized in the olfactory epithelia of lacustrine sockeye salmon (O. nerka) by molecular biological and histochemical techniques. Two cDNAs encoding salmon OMP were isolated and sequenced.These cDNAs both contained a coding region encoding 173 amino acid residues, and the molecular mass of the two proteins was calculated to be 19,581.17 and 19,387.11 Da, respectively. Both amino acid sequences showed marked homology (90%). The protein and nucleotide sequencing demonstrates the existence of high-level homology between 2 salmon OMPs and those of other teleosts. By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. By immunohistochemistry using a specific antibody to salmon OMP, OMP-immunoreactivities were noted in the cytosol of those neurons. The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon.

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“…27, 28, 30). On the other hand, an ATP-dependent system to excrete exogen substances has been found in teleost (Yanagi et al 2004;Hirose and Nakada 2010). Accordingly, the increased ATP content found in the present study may be linked with increased energy demands resulting from an increased excretion of exogen substances as a result of a poor physical condition.…”

Section: -2 Development Of Techniques To Monitor the Physical Condimentioning

confidence: 56%

Studies on Improvement of Seed Production Techniques in Salmonids and Osmerids

Mizuno¹

2012

Aqua-BioSci. Monogr. (ABSM)

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anadromous and stream-resident types. The anadromous type migrates to the ocean in spring after spending 1 or 2 years in the streams. Before the seaward migration, they undergo smoltification, a series of behavioral, morphological, physiological and biochemical changes controlled by multiple hormonal pathways (Hoar 1988) that transform the freshwater parr into a seaward-migratory smolt. After 1 year of life in the ocean, the fish return to their natal stream in spring and spawn in autumn (Kubo 1980;Machidori and Kato 1984;Kato 1991). For the artificial propagation of masu salmon, hatchery-reared (hatchery) juveniles, originating from artificial insemination and fed a commercial food in hatcheries, are released into streams (Mayama 1992). Effects of masu salmon propagation, which are expressed as the recovery rate of released hatchery seed, are of 0.41 to 2.12% (Miyakoshi 2006), while the recovery rate of chum salmon artificial propagation was >10% (Taya 1989). In other Salmon and Freshwater Fisheries Research Institute Fisheries Research Department Local Independent Administrative Agency Hokkaido Research Organization 3-373, Kitakashiwagi, Eniwa, Hokkaido 061-1433, Japan e-mail: mizuno-shinya@hro.or.jp Abstract Anadromous salmonids and osmerids are artificially propagated in Japan by release of their seeds. However, there are many unsolved problems in the techniques of their propagation. In the present monograph, studies on the improvement of seed production techniques in 4 fishes (masu salmon Oncorhynchus masou, chum salmon O. keta, shishamo smelt Spirinchus lanceolatus and Japanese smelt Hypomesus nipponensis) were outlined. Techniques to evaluate dorsal fin pigmentation during smoltification as an external seed quality, and to improve seed quality of hatchery-reared fish, and the discovery of metabolic problems in hatchery-reared fish were described in yearling masu salmon. In underyearling masu salmon, techniques to evaluate nutritional conditions using kidney melano-macrophage density was developed, and applied to the evaluation of the nutritional condition in hatchery-reared fish after release. In chum salmon fry, the development of techniques to monitor the physical condition and to find its appropriate culture conditions was reviewed. In egg cultures of shishamo and Japanese smelt, techniques to eliminate egg adhesiveness with treatments of kaolin or scallop shell powder suspension were established in order to improve hatching rates. In addition, the appropriate embryogenetic stage for the release of shishamo smelt embryos was demonstrated. Consequently, this monograph reveals that these techniques contribute directly to the development of artificial propagation in some salmonids and osmerids. Studies on Improvement of Seed Production Techniques in Salmonids and Osmerids Shinya Mizuno

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Immunohistochemical demonstration of salmon olfactory glutathione S-transferase class pi (N24) in the olfactory system of lacustrine sockeye salmon during ontogenesis and cell proliferation

Cited by 21 publications

References 24 publications

Diversity in the olfactory epithelium of bony fishes: Development, lamellar arrangement, sensory neuron cell types and transduction components

Diversity in the olfactory epithelium of bony fishes: Development, lamellar arrangement, sensory neuron cell types and transduction components

Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)

Studies on Improvement of Seed Production Techniques in Salmonids and Osmerids

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