Immunohistochemical demonstration of serotonin nerve fibers in the subthalamic nucleus of the rat, cat and monkey

Mori, Shigeki; Takino, Tatsuro; Yamada, Hisao; Sano, Yutaka

doi:10.1016/0304-3940(85)90566-x

Cited by 44 publications

(39 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, stimulation of ␣1-AR has a direct excitatory effect on SNc DA neurons (Grenhoff et al, 1995) that, in return, exert a direct excitatory modulation of STN neurons (Cragg et al, 2004). ␣1-ARs are highly expressed in the dorsal raphe nucleus that sends 5-HT projections to the STN (Mori et al, 1985;Lavoie and Parent, 1990). 5-HT induces both excitation and inhibition in the STN (Stanford et al, 2005).…”

Section: Effects Of ␣1 Noradrenergic Agents On Stn Neuronal Activitymentioning

confidence: 99%

Noradrenergic Modulation of Subthalamic Nucleus Activity: Behavioral and Electrophysiological Evidence in Intact and 6-Hydroxydopamine-Lesioned Rats

Belujon¹,

Bézard²,

Taupignon³

et al. 2007

J. Neurosci.

View full text Add to dashboard Cite

The subthalamic nucleus (STN) plays a key role in the pathophysiology of Parkinson's disease. The modulation of the STN by norepinephrine, however, is unknown. The present study aims at characterizing the effects of systemic administration of noradrenergic agents on locomotor activity and on in vivo extracellularly recorded STN neuronal activity in intact and 6-hydroxydopamine (6-OHDA)-lesioned rats. Using selective agonists and antagonists of ␣1 and ␣2 adrenergic receptors (ARs), we show that STN neurons have functional ␣1-and ␣2-AR controlling STN firing with an impact on locomotor activity. We further demonstrate that those systemic effects are supported, at least in part, by a direct modulation of STN neuronal activity, using patch-clamp recordings of STN neurons in brain slices. These findings support the premise that hypokinesia is associated with an increased STN neuronal activity, and that improvements of parkinsonian motor abnormalities are associated with a decrease in STN activity. Our data challenge assumptions about the role of ␣1-AR and ␣2-AR in the regulation of STN neurons in both intact and 6-OHDA-lesioned rats and further ground the rationale for using ␣2-AR noradrenergic antagonists in Parkinson's disease, albeit via an unexpected mechanism.

show abstract

Section: Effects Of ␣1 Noradrenergic Agents On Stn Neuronal Activitymentioning

confidence: 99%

Noradrenergic Modulation of Subthalamic Nucleus Activity: Behavioral and Electrophysiological Evidence in Intact and 6-Hydroxydopamine-Lesioned Rats

Belujon¹,

Bézard²,

Taupignon³

et al. 2007

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The STN receives a dense 5HT-containing innervation that arises from the dorsal raphe nucleus (Canteras et al, 1990;Mori et al, 1985). Furthermore, the STN has been shown to express several 5HT receptor subtypes, * Author for correspondence: Steven W, Johnson, MD, PhD, Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97207 USA, Section Editor: Dr. Yoland Smith (neuropharmacology) Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication.…”

Section: Introductionmentioning

confidence: 99%

“…The STN receives a dense 5HT-containing innervation that arises from the dorsal raphe nucleus (Canteras et al, 1990;Mori et al, 1985). Furthermore, the STN has been shown to express several 5HT receptor subtypes, including 5HT 1A , 5HT 1B , 5HT 2C , and 5HT 4 (Pompeiano et al, 1994;Wright et al, 1995;Bruinvels et al, 1993;Compan et al, 1996).…”

mentioning

confidence: 99%

5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro

Shen

Johnson

2008

Neuroscience

View full text Add to dashboard Cite

The subthalamic nucleus (STN) plays a pivotal role in normal and abnormal motor function. We used patch pipettes to study effects of 5-hydroxytryptamine (5HT) on synaptic currents evoked in STN neurons by focal electrical stimulation of rat brain slices. 5HT (10 μM) reduced glutamatemediated excitatory postsynaptic currents (EPSCs) by 35 ± 4%. However, a much higher concentration of 5HT (100 μM) was required to inhibit GABA-mediated inhibitory postsynaptic currents (IPSCs) to a comparable extent. Concentration-response curves showed that the 5HT IC 50 for inhibition of IPSCs (20.2 μM) was more than 5-fold greater than the IC 50 for inhibition of EPSCs (3.4 μM). The 5HT-induced reductions in EPSCs and IPSCs were accompanied by increases in paired-pulse ratios, indicating that 5HT acts presynaptically to inhibit synaptic transmission. The 5HT1B receptor antagonist NAS-181 significantly antagonized 5HT-induced inhibitions of EPSCs and IPSCs. These studies show that 5HT inhibits synaptic transmission in the STN by activating presynaptic 5HT 1B receptors.Keywords presynaptic inhibition; EPSC; IPSC; 5-hydroxytryptamine; serotonin; brain slice; patch clampThe subthalamic nucleus (STN) is a basal ganglia structure comprised of glutamate-containing neurons that project to the two output nuclei of the basal ganglia: substantia nigra pars reticulata and globus pallidus interna (Albin et al., 1995). By way of its glutamatergic projections, the STN plays an important role in normal and pathological motor behaviors. For example, excessive burst firing of STN neurons is thought to contribute to the akinesia of Parkinson's disease (Benedetti et al., 2004;Wichmann et al., 1994). The importance of the STN in the control of movement is also evidenced by the effective therapeutic effects of surgical lesions in an animal model of Parkinson's disease (Bergman et al., 1990) and high frequency STN stimulation in Parkinson's disease patients (Krack et al., 1998).Among the neurotransmitter systems that regulate STN neuronal activity, the actions of 5-hydroxytryptamine (5HT) have yet to be fully investigated. The STN receives a dense 5HT-containing innervation that arises from the dorsal raphe nucleus (Canteras et al., 1990;Mori et al., 1985). Furthermore, the STN has been shown to express several 5HT receptor subtypes, * Author for correspondence: Steven W, Johnson, MD, PhD, Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97207 USA, Section Editor: Dr. Yoland Smith (neuropharmacology) Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. (Pompeiano et al., 1994;W...

show abstract

“…The STN is innervated by rich varicose serotonin (5-HT) fibers (Bobillier et al 1976;Lavoie and Parent 1990;Moore et al 1978;Mori et al 1985;Palkovits et al 1974;Steinbusch 1981), which originate mainly from the neurons in the dorsal raphe nucleus (Bobillier et al 1976;Lavoie and Parent 1990). The serotonergic neurons in the dorsal raphe nucleus have been implicated in various aspects of motor control (Steinfels et al 1983).…”

Section: Introductionmentioning

confidence: 99%

Modulation of Spontaneous Firing in Rat Subthalamic Neurons by 5-HT Receptor Subtypes

Xiang

Wang²,

Kitai³

2005

Journal of Neurophysiology

View full text Add to dashboard Cite

Xiang, Zixiu, Lie Wang, and Stephen T. Kitai. Modulation of spontaneous firing in rat subthalamic neurons by 5-HT receptor subtypes. J Neurophysiol 93: 1145-1157, 2005 doi:10.1152/jn.00561. 2004. The subthalamic nucleus (STN) is considered to be one of the driving forces in the basal ganglia circuit. The STN is innervated by serotonergic afferents from the raphe nucleus and expresses a variety of 5-HT receptor subtypes. We investigated the effects of 5-HT and 5-HT receptor subtype agonists and antagonists on the firing properties of STN neurons in rat brain slices. We used cell-attached, perforated-patch, and whole cell recording techniques to detect changes in firing frequency and pattern and electrical membrane properties. Due to the depolarization of membrane potential caused by reduced potassium conductance, 5-HT (10 M) increased the firing frequency of STN neurons without changing their firing pattern. Cadmium failed to occlude the effect of 5-HT on firing frequency. 5-HT had no effect on afterhyperpolarization current. These results indicated that the 5-HT action was not mediated by high-voltageactivated calcium channel currents and calcium-dependent potassium currents. 5-HT had no effect on hyperpolarization-activated cation current (I H ) amplitude and voltage-dependence of I H activation, suggesting that I H was not involved in 5-HT-induced excitation. The increased firing by 5-HT was mimicked by 5-HT 2/4 receptor agonist ␣-methyl-5-HT and was partially mimicked by 5-HT 2 receptor agonist DOI or 5-HT 4 receptor agonist cisapride. The 5-HT action was partially reversed by 5-HT 4 receptor antagonist SB 23597-190, 5-HT 2 receptor antagonist ketanserin, and 5-HT 2C receptor antagonist RS 102221. Our data indicate that 5-HT has significant ability to modulate membrane excitability in STN neurons; modulation is accomplished by decreasing potassium conductance by activating 5-HT 4 and 5-HT 2C receptors.

show abstract

Immunohistochemical demonstration of serotonin nerve fibers in the subthalamic nucleus of the rat, cat and monkey

Cited by 44 publications

References 9 publications

Noradrenergic Modulation of Subthalamic Nucleus Activity: Behavioral and Electrophysiological Evidence in Intact and 6-Hydroxydopamine-Lesioned Rats

Noradrenergic Modulation of Subthalamic Nucleus Activity: Behavioral and Electrophysiological Evidence in Intact and 6-Hydroxydopamine-Lesioned Rats

5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro

Modulation of Spontaneous Firing in Rat Subthalamic Neurons by 5-HT Receptor Subtypes

Contact Info

Product

Resources

About