Immunohistochemical Detection of c-myc Products in Colorectal Cancer and Proliferative Cell Rate

Yamaguchi, Akio; Ninomiya, Itasu; Ishida, Tetsuya; Nishimura, Genichi; Kanno, Masahiro; Yonemura, Yutaka; Miwa, Kouichi; Miyazaki, Itsuo; Matsukawa, Shigeru

doi:10.1159/000227008

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…These results suggest that the relationship between cell proliferation and expression of c-myc is not clear-cut and other regulatory mechanisms are probably involved. The present results confirm previous results in that poor histological differentiation and expression of c-myc are interrelated (Kotake et al, 1990;Yamaguchi et al, 1992;Lanigan et al, 1993). Stage of disease and expression of Myc were not interrelated, which is at variance with previous results in renal adenocarcinomas (Lanigan et al, 1993).…”

Section: Expression Of Bcl-2supporting

confidence: 93%

“…Amplification or overexpression of c-myc has been previously related to cell proliferation in human neoplasms (Munzel et al, 1991;Melhem et al, 1992;Yamaguchi et al, 1992) albeit the results are variable. In this study cytoplasmic expression of c-myc was independent of mitotic index while nuclear expression was related to low proliferation rate.…”

Section: Expression Of Bcl-2mentioning

confidence: 99%

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Expression of tumour-suppressor gene Rb, apoptosis-suppressing protein Bcl-2 and c-Myc have no independent prognostic value in renal adenocarcinoma

Lipponen

Eskelinen²,

Syrjänen³

1995

Br J Cancer

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Summary The expression of retinoblastoma (Rb), c-Myc and Bcl-2 proteins was studied by immunohistochemical methods in 104 cases of renal adenocarcinoma. One tumour was completely negative for Rb protein and altered expression pattern was detected in 36% of cases. A low fraction of Rb-positive nuclei was related to high grade (P = 0.016) and high mitotic index (P = 0.012). Twenty-eight per cent of the tumours expressed c-Myc in cancer cell nuclei and 87% showed cytoplasmic positivity. Cytoplasmic expression of c-Myc was related to high grade (P= 0.002), while nuclear expression of c-Myc was related to small tumour diameter (P = 0.034), low T category (P = 0.04), low mitotic index (P = 0.019) and expression of c-ErbB-2 (P = 0.0007). Overexpression of c-myc predicted favourable outcome in MO tumours (P = 0.0157). Bcl-2 was expressed in 20% of tumours and it was related to small tumour size (P<0.0001), low T category (P<0.0001), lack of venous invasion (P= 0.008), node negativity (P = 0.015) and absence of metastasis (P = 0.017). In multivariate analysis the expression of Rb, Bcl-2 and c-Myc had no independent prognostic value over T category (P<0.001), mitotic index (P = 0.008) and combined nuclear grade (P = 0.056).

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Section: Expression Of Bcl-2supporting

confidence: 93%

Section: Expression Of Bcl-2mentioning

confidence: 99%

Expression of tumour-suppressor gene Rb, apoptosis-suppressing protein Bcl-2 and c-Myc have no independent prognostic value in renal adenocarcinoma

Lipponen

Eskelinen²,

Syrjänen³

1995

Br J Cancer

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“…c‐Myc is a nuclear protein which is generally associated with the nuclear matrix; 21–23 however, in our samples c‐Myc immunoreactivity appeared mostly confined to the cytoplasm even though, occasionally, positive nuclei could be observed. Cytoplasmic localization of c‐Myc has been attributed by some authors to the methods used for tissue fixation and processing 24,25 . There are no indications that this could be the case in our study because fixation either with methanol‐acetone or paraformaldehyde did not change the pattern of the immunoreactivity.…”

Section: Discussioncontrasting

confidence: 53%

c-Myc expression in human anagen hair follicles

Rumio

Donetti

Imberti

et al. 2000

British Journal of Dermatology

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The hair follicle represents a very attractive organ system for studying the precise balance between cell proliferation, growth, differentiation, and death of cells, because it periodically and regularly regenerates, retaining its morphogenetic signals throughout its life. One of the most intriguing oncogenes which is able to induce both cell growth and apoptosis, depending upon the environmental conditions, is c-myc. The aim of the present study was to investigate its presence and localization in human hair follicles by immunohistochemistry and immunofluorescence. Our observations demonstrated the consistent presence of two clusters of cMyc-expressing cells in anagen follicles, located in two annular regions of the inner root sheath, at the border between cells characterized by putative trichohyalin granules and cells which are keratinized. The lower group belongs to Henle's layer, while the upper group belongs to Huxley's layer. cMyc oncoprotein seems to favour apoptosis/differentiation and may be a marker for terminal differentiation of trichocytes, at least in the inner root sheath. Our findings agree with the interpretation that the complex morphology of the hair follicle reflects its complex function; the extrusion of a highly organized multicellular structure, the hair shaft, driven by another highly organized multicellular structure, the inner root sheath.

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“…Physiological conditions such as the proliferation of colonic epithelial crypts" is not related to myc expression, while in colonic cancer, cell proliferation and the expression of myc protein are interrelated. 34 The results of previous studies of transitional cell bladder tumours2' and in other urological neoplasia" suggest that poor histological differentiation, cell proliferation, and expression of c-myc are interrelated. The present study confirms previous results in that cell proliferation, DNA aneuploidy, high histological grade, and expression of c-myc were interrelated.…”

Section: Discussionmentioning

confidence: 96%

Expression of c‐myc protein is related to cell proliferation and expression of growth factor receptors in transitional cell bladder cancer

Lipponen

1995

The Journal of Pathology

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Archival biopsy specimens from transitional cell bladder tumours (n = 185) were analysed immunohistochemically for expression of c-myc protein. The results were compared with histopathological and clinical parameters and survival. Forty-three per cent of the tumours were negative for c-myc protein and weak, moderate, or strong cytoplasmic expression was found in 34, 14, and 9 per cent of cases, respectively. Nuclear positivity for c-myc protein was detected in 35 per cent of tumours and nuclear positivity was related to overexpression of c-erb B-2 (P = 0.01) and a high proportion of nuclei were also positive for p53 oncoprotein (p < 0.05). Cytoplasmic expression of c-myc protein was related to histological grade (P = 0.005), papillary status (P = 0.007), the S-phase fraction (P = 0.008), the mitotic index (P = 0.021), overexpression of epidermal growth factor receptor (P = 0.045), and c-erb B-2 (P = 0.17). Expression of c-myc protein was not significantly related to the progression of tumours and it had no prognostic value in survival analysis. Independent predictors were the T-category (P < 0.001), papillary status. (P = 0.001), and S-phase fraction (P = 0.061). The results show that while c-myc gene product participates in growth regulation of human bladder cancer cells, it has no independent prognostic significance.

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Immunohistochemical Detection of c-myc Products in Colorectal Cancer and Proliferative Cell Rate

Cited by 7 publications

References 12 publications

Expression of tumour-suppressor gene Rb, apoptosis-suppressing protein Bcl-2 and c-Myc have no independent prognostic value in renal adenocarcinoma

Expression of tumour-suppressor gene Rb, apoptosis-suppressing protein Bcl-2 and c-Myc have no independent prognostic value in renal adenocarcinoma

c-Myc expression in human anagen hair follicles

Expression of c‐myc protein is related to cell proliferation and expression of growth factor receptors in transitional cell bladder cancer

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