Immunohistochemical Detection of CD34, E-cadherin, Claudin-1, Glucose Transporter 1, Laminin, and Protein Gene Product 9.5 in 28 Canine and 8 Feline Meningiomas

Abstract

The variation in histologic pattern of meningiomas can make their diagnosis challenging. The immunohistochemical profile of 28 canine and 8 feline meningiomas was examined. Tumor types included anaplastic (6 dogs), angiomatoid (1 cat), fibroblastic (3 dogs, 1 cat), meningothelial (1 dog), microcystic (2 dogs), myxoid (3 dogs), psammomatous (4 cats), and transitional (13 dogs, 2 cats). The authors compared the expression of novel markers (CD34, E-cadherin, claudin-1, glucose transporter 1 [GLUT-1], laminin, and protein gene product [PGP] 9.5) with published markers (cytokeratins, glial fibrillary acidic protein [GFAP], progesterone receptor, S100, and vimentin). Neoplastic cells were immunohistochemically positive for vimentin in 100% of the meningiomas; CD34, 94%; GLUT-1, 86%; E-cadherin, 81%; S100, 75%; laminin, 72%; claudin-1, 60%; PGP 9.5, 55%; progesterone receptor, 44%; pancytokeratins, 39%; cytokeratins 8/18, 17%, and GFAP in 9%. Ki67 index did not correlate well with mitotic index. Based on these results and those in the human literature, immunohistochemistry for vimentin, CD34, and E-cadherin is proposed to support a diagnosis of meningioma. Immunohistochemistry for claudin-1, albeit of only moderate to low sensitivity in canine and feline meningiomas, may help to distinguish meningioma from some mesenchymal neoplasms involving the brain and associated structures, such as schwannomas, which in humans express claudin-1 poorly or not at all. Further studies with CD34, E-cadherin, and claudin-1 in canine and feline tumors that may mimic meningiomas are needed to determine the adequacy of this approach.

Spinal Meningeal Oligodendrogliomatosis in Two Boxer Dogs

“…Canine meningiomas of various histological patterns have been reported to be immunopositive for CD34 and E-cadherin in 92% and 79% of dogs, respectively. 9 The growth pattern of the oligodendroglial tumor in these 2 dogs is unusual. However, widespread dissemination of oligodendroglial tumors into the leptomeninges is recognized in humans.…”

Advances in Diagnostic and Treatment Modalities for Intracranial Tumors

“…Similarly, overexpression of MMP‐2 and ‐9, E‐cadherin, claudin‐1, IL‐6, and cyclooxygenase‐2 has been documented in canine and feline meningiomas,121, 122, 123, 124, 125, 126 and MMP‐2/9 has been identified in canine gliomas and choroid plexus tumors 125. Overexpression of surface markers previously defined in human tumors, suitable for tumor targeting strategies, has been demonstrated for IL‐13RA2, EphA2, and alpha3‐beta1 integrin in a variety of canine brain tumors (Fig 4).…”

Comparison of CD34, CD31, and Factor VIII–Related Antigen Immunohistochemical Expression in Feline Vascular Neoplasms and CD34 Expression in Feline Nonvascular Neoplasms

“…Immunohistochemistry was performed on all formalinfixed, paraffin-embedded (FFPE) vascular neoplasms using antibodies to factor VIII-related antigen (FVIII-ra), CD31, and CD34 according to established protocols 21,23 with minor modifications (Table 1). Immunohistochemistry using anti-CD34 antibody was also performed on all FFPE nonvascular neoplasms.…”