Immunohistochemical Detection of Dendritic Cell Markers in Cattle

Abstract

Dendritic cells (DCs) are ''professional'' antigen-presenting cells with a critical role in the regulation of innate and adaptive immune responses and thus have been considered of great interest in the study of a variety of infectious diseases. The objective of this investigation was to characterize the in vivo distribution of DCs in bovine tissues by using potential DC markers to establish a basis for the study of DCs in diseased tissues. Markers evaluated included MHCII, CD208, CD1b, CD205, CNA.42, and S100 protein, the latter 2 being expressed by follicular dendritic cells whose origin and role are different from the rest of hematopoietic DCs. Paraffin wax-embedded tissues from 6 healthy Friesian calves were subjected to the avidin-biotin-peroxidase method, and the most appropriate fixatives, dilutions, and antigen retrieval pretreatments were studied for each of the primary antibodies. The most significant results included the localization of CD208-positive cells not only in the T zone of lymphoid organs but also within lymphoid follicles; CD1b-positive cells were mainly found in thymus and interfollicular areas of some lymph nodes; cells stained with anti-CD205 antibody were scarce, and their location was mainly in nonlymphoid tissues; and CNA.42-and S100 proteinpositive cells localized in primary lymphoid follicles and light zones of germinal centers, although showing differences in the staining pattern. Furthermore, MHCII was established as one of the most sensitive markers for any DC of hematopoietic origin. These results increase our understanding of DC immunolabeling and will help in future DC studies of both healthy and diseased tissues.

Immunohistochemical study on roe deer haemal nodes

“…It was detected that IHC studies were carried out in the lymph node of rat with S100 protein [6], Romero-Palomo et al [13] has employed polyclonal rabbit anti-S100 primary antibody as dendritic cell marker in the lymph node of cattle. There is also a comparative study on S100 protein-immunoreactive cells in the lymph nodes of guinea pig, dog, cat, horse, pig, goat and cow [14].…”

Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1

“…Thymic DCs are considered the major cell population responsible for the process of immunologic central tolerance, by participating in the deletion of self‐reactive developing T cells (Ardavin, ) and the induction of natural regulatory T cells (Proietto et al., ). For this reason, we studied this type of cells by immunolabelling against two molecules (CD1b and CD208) previously reported as DC markers in cattle (Romero‐Palomo et al., ).…”

Evaluation of a panel of antibodies for the immunohistochemical identification of immune cells in paraffin-embedded lymphoid tissues of new- and old-world camelids

“…Antibodies against CD205 and CD208 antigen were applied for the detection of DCs, as previously reported in cattle (Romero-Palomo et al, 2013), sheep (Åkesson et al, 2008) andhumans (de Saint-Vis et al, 1998;Geijtenbeek et al, 2000). Only CD208 (synonym dendritic cell lysosomal-associated membrane protein [DC-LAMP]) revealed specific immunoreaction on few histiocytic cells in camelid tissue ( Fig.…”