Immunohistochemical detection of human telomerase reverse transcriptase in oral cancer and pre-cancer

Palani, Jayanthi; Lakshminarayanan, Vidya; Ranganathan, Kannan

doi:10.4103/0970-9290.84281

Cited by 13 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Use of IHC is a useful, reliable method of localizing the hTERT protein in tissue sections. [ 12 15 ] Telomerase activity is conventionally studied using modified polymerase chain reaction, the telomeric repeat amplification protocol assay. This technique does not permit cellular localization of telomerase activity which is better demonstrated with techniques likes IHC/ in situ hybridization.…”

Section: Discussionmentioning

confidence: 99%

“…Telomerase activity is up-regulated in many tumors and studies have shown that increased telomerase expression is an early event in oral carcinogenesis. [ 7 8 9 10 11 12 13 14 ] The aim of our study was to ascertain the expression of hTERT as an indicator of telomerase activity in oral epithelia dysplasia and oral squamous cell carcinoma (OSCC) using immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissues.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of human telomerase reverse transcriptase protein in oral epithelial dysplasia and oral squamous cell carcinoma: An immunohistochemical study

Raghunandan

Sanjai

Kumaraswamy

et al. 2016

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Background:Telomerase is an RNA-dependent DNA polymerase that synthesizes TTAGGG telomeric DNA sequences and almost universally provides the molecular basis for unlimited proliferative potential. The telomeres become shorter with each cycle of replication and reach a critical limit; most cells die or enter stage of replicative senescence. Telomere length maintenance by telomerase is required for all the cells that exhibit limitless replicative potential. It has been postulated that reactivation of telomerase expression is necessary for the continuous proliferation of neoplastic cells to attain immortality. Use of immunohistochemistry (IHC) is a useful, reliable method of localizing the human telomerase reverse transcriptase (hTERT) protein in tissue sections which permits cellular localization. Although there exists a lot of information on telomerase in oral cancer, little is known about their expression in oral epithelial dysplasia and their progression to oral squamous cell carcinoma (OSCC) compared to normal oral mucosa. This study addresses this lacuna.Aims:To compare the expression of hTERT protein in oral epithelial dysplasia and OSCC with normal oral mucosa by Immunohistochemical method.Subjects and Methods:In this preliminary study, IHC was used to detect the expression of hTERT protein in OSCC (n = 20), oral epithelial dysplasia (n = 21) and normal oral mucosa (n = 10). The tissue localization of immunostain, cellular localization of immunostain, nature of stain, intensity of stain, percentage of cells stained with hTERT protein were studied. A total number of 100 cells were counted in each slide.Statistical Analysis:All the data were analyzed using SPSS software version 16.0. The tissue localization, cellular localization of cytoplasmic/nuclear/both of hTERT stain, staining intensity was compared across the groups using Pearson's Chi-square test. The mean percentage of cells stained for oral epithelial dysplasia, OSCC and normal oral mucosa were compared using analysis of variance (ANOVA). A P < 0.05 was considered to be statistically significant.Results:The mean hTERT positive cells in the study groups were as follows, 62.91% in normal oral mucosa samples, 77.06% in oral epithelial dysplasia cases, and 81.48% in OSCC. In 61.9% of oral epithelial dysplasia and 65% of OSCC in our study, staining was visualized within the nucleus predominantly in the dot like pattern. There was a statistically significant difference in the nature of nuclear stain between oral epithelial dysplasia and OSCC (P = 0.023).Conclusions:Our results suggests that the mean percentage of cells showing hTERT expression steadily increased from normal oral mucosa to oral epithelial dysplasia to OSCC. The steady trend of increase in the percentage of cells was evident in different grades of oral epithelial dysplasia group and OSCC. The nature of hTERT staining did show variations among the three groups and promise to be a potential surrogate marker for malignant transformation. Further studies using IHC on larger sample size and clinica...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of human telomerase reverse transcriptase protein in oral epithelial dysplasia and oral squamous cell carcinoma: An immunohistochemical study

Raghunandan

Sanjai

Kumaraswamy

et al. 2016

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

show abstract

“…The malignant transformation rate for leukoplakia ranges from 15% to 20%, while transformation rates as high as 7.6% over a 10‐year period have been reported for OSF . Many markers including cytokeratins and human telomerase reverse transcriptase (hTERT) have been studied and found to be altered in malignant transformation …”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical detection of p53 and p63 in oral squamous cell carcinoma, oral leukoplakia, and oral submucous fibrosis

Varun

Ranganathan

Rao

et al. 2013

J of Invest & Clin Dent

View full text Add to dashboard Cite

show abstract

“…Detection of the telomerase activity in tissue by immunohistochemical methods is recent and reliable method in human tumors. Telomerase expressed in cytoplasm and nucleus of the tumoral cells [38][39][40] . In this study we used this technique in ENA and our results showed that telomerase expression in tumor tissue was increased compared to normal nasal tissue.…”

Section: Discussionmentioning

confidence: 99%

Keçilerin Enzootik Intranazal Tümörlerinde Telomeraz Enziminin Düzeyinin Belirlenmesi

Şahinduran¹,

Özmen²,

Kiyici³

2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Enzootic intranasal adenocarcinoma (ENA) of goats are contagious tumoral disease that characterized occurrence of tumoral masses in nasal cavity of the animals. The etiological agent of the disease is retrovirus and the disease cause high economical loses in goat industry. Retroviruses, respectively inducing lung or nasal adenocarcinomas. The true economic impact of ENA is not known because affected animals are given insights from the herd before actual diagnosis, and suspected disease incidence is rarely reported. Telomerase regulates the proliferative capacity of cells and this enzyme has critical role in tumor progression. The aim of this study is to evaluate telomerase activity in serum and tumoral tissue in goat with ENA. For this aim 11 goat's serum were analyses for telomerase activity. Tumoral tissue sections of totally 14 death goats were examined by immunohistochemically for telomerase expression. In addition, 10 normal goat's serum and nasal tissues that belong slaughtered healthy animals used as control. This study showed that increased serum and tissue telomerase activity in goats with ENA comparing to controls. Also our results showed that telomerase activity becomes a useful prediction marker for tumor development in infected but clinically healthy goats in infected flocks.

show abstract

Immunohistochemical detection of human telomerase reverse transcriptase in oral cancer and pre-cancer

Abstract: There was increased expression of hTERT protein in OSCC and leukoplakia samples when compared to normal oral mucosa. The cellular localization, LI and LS in OSF were significantly different from OSCC and leukoplakia.

Cited by 13 publications

References 0 publications

Expression of human telomerase reverse transcriptase protein in oral epithelial dysplasia and oral squamous cell carcinoma: An immunohistochemical study

Expression of human telomerase reverse transcriptase protein in oral epithelial dysplasia and oral squamous cell carcinoma: An immunohistochemical study

Immunohistochemical detection of p53 and p63 in oral squamous cell carcinoma, oral leukoplakia, and oral submucous fibrosis

Keçilerin Enzootik Intranazal Tümörlerinde Telomeraz Enziminin Düzeyinin Belirlenmesi

Contact Info

Product

Resources

About