Immunohistochemical detection of keratin proteins in salivary gland ducts of mammals.

Takai, Yoshiaki; Noda, Yoko; Sumitomo, Shinichiro; Kawamura, Kazutaka; Mori, Masahiko

doi:10.1267/ahc.18.353

Cited by 19 publications

(14 citation statements)

References 15 publications

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“…In accordance with previous immunohistochemical studies of keratin distribution in salivary glands of mammals [7,10], the present study revealed that, the cytoplasm of all ductal cells in both the mandibular salivary gland and the pancreas including centroacinar cells were strongly immunopositive to the cytokeratin. However, the cells forming secretory endpeices were negatively reacted.…”

Section: Discussionsupporting

confidence: 92%

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Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Elewa¹,

Elsayed²,

Mohamed³

et al. 2017

J Vet Sci Technol

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The centroacinar cells (CACs) are a specialized ductal pancreatic cell. Recently, it has been revealed that such cells showed a progenitor function in the pancreas of adult mouse and zebrafish. However, no information about its occurrence in other exocrine glands specially in farm animals. Cytokeratins immunohistochemistry was used to detect ductal segments of the submandibular glands and the pancreas including CACs in human and rat. In the present study, the detection of cytokeratins in the normal goat pancreas (a serous type gland) and mandibular salivary gland (a mixed type gland, consisted of mucous tubules capped with serous demilunes) was investigated using a standard immunoperoxidase technique on paraffin sections. In the goat pancreas and mandibular salivary gland, cytokeratins were found in all duct cells including the CACs of the pancreas. Interestingly in the mandibular salivary glands, some immunoreacted cells with immunopositive cytoplasmic extensions were frequently observed between the cytoplasm of the mucous cells, especially near the lumina of the mucous tubules. We postulate that these cells could be centroacinar-like cells. Therefore, from these findings, it can be concluded that these cells could provide a supportive cytoskeleton to maintain cellular shape and avoid the collapse of the mucous cells during mucous secretion. Additionally, a regenerative function for such cells could be hypothesized. Furthermore, the keratin demonstration could be used as a marker for ductal cells and is a useful aid in the detection of lesions derived from gland ductal cells in goat. Further research is necessary to investigate the expression of keratins in caprine glandular tumors as well as the regenerative ability of these centroacinar-like cells in goat mandibular salivary glands injury.

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Section: Discussionsupporting

confidence: 92%

“…Furthermore, cells from the excretory ducts of the rat pancreas including the centroacinar cells (CACs) showed immunopositive reaction for different cytokeratins antibodies [8,9]. However, little has been elucidated so far concerning their distribution in farm animals, especially in goats [10].…”

Section: Introductionmentioning

confidence: 99%

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Elewa¹,

Elsayed²,

Mohamed³

et al. 2017

J Vet Sci Technol

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“…Ductal Basal Cell: Ductal Basal Cells in striated and excretory ducts of human salivary glands have been estimated in terms of keratin immunohistochemistry (38,50,81,84), and these basal cells also have important stem cells in neoplastic transformation of pleomorphic adenomas (72,95) tion with secretion of calcitonin. Other Factors: It has been reported that salivary glands in human and animals contain and secrete other growth factors and other growth factor-related substances identified by biological and biochemical studies, however cellular origin of these substances in salivary glands is still unknown.…”

Section: Calcitonin (Cat)mentioning

confidence: 99%

Biologically active peptides in the submandibular gland. Role of the granular convoluted tubule.

Mori¹,

Takai²,

Kunikata³

1992

Acta Histochem. Cytochem

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The granular convoluted tubule segment (GCT) of the rodent submandibular gland is a unique structure and consists of granular cell, dark granular cell, pillar cell, and transitional cell. The granular cells contain secretory granules and synthesize biologically active peptides and related processing enzymes; EGF, NGF, kallikrein and renin, erythropoietin, atrial natriuretic peptides, large mobile protein as cystain and serin proteinase inhibitors, S-100 protein, glucagon and somatostatin.Granular cells may have a multiproduction system for growth factors or biologically active peptides for related processing proteinases as well as proteinase inhibitors which are secreted by a-adrenergic stimulants. Pillar cells contain S-100 protein, neuron specific enolase and other nerve related materials, and involve regulation of the nerverelated system as neurotransmitter-like or paraneuron-like properties. Transitional cells show combined properties of granular convoluted tubule and striated duct, and may participate in secretion and reabsoorption. Granular intercalated duct cells show similar category of granular cells in GCT segment.

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“…In the present study, ductal epithelia and acinar cells, either serous or mucous, of normal glands indicated negative staining as did duct-like structures in obstructive sialadenitis. Keratin staining is strongly evident in epithelial architecture, i.e., normal ductal segments of salivary glands and duct-like structures of sialadenitis (2,3,6,12). This finding suggests that the duct-like structures in obstructive salivary lesions have less tendency to terminal keratinization.…”

Section: Discussionmentioning

confidence: 89%

Expression of involucrin in human salivary gland lesions and tumors.

Sumitomo

Kumasa

Iwai

et al. 1986

Acta Histochem. Cytochem

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Immunohistochemical detection of keratin proteins in salivary gland ducts of mammals.

Cited by 19 publications

References 15 publications

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Detection of an Intermediate Filament Protein in the Pancreas and Mandibular Salivary Gland of the Goat (Capra hircus): An Immunocytochemical Study

Biologically active peptides in the submandibular gland. Role of the granular convoluted tubule.

Expression of involucrin in human salivary gland lesions and tumors.

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