2012
DOI: 10.1292/jvms.11-0336
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Immunohistochemical Detection of Polyunsaturated Fatty Acid Oxidation Markers in Acetaminophen-Induced Liver Injury in Rats

Abstract: ABSTRACT. To clarfity whether polyunsaturated fatty acid (PUFA) oxidation is involved in the mechanism of acetaminophen (APAP)-induced apoptotic cell death, the production and localization of PUFA oxidation markers N  -propanoyl-modified lysine, N  -hexanoylmodified lysine, 4-hydroxyhexenal-modified histidine and crotonaldehyde-modified lysine were evaluated in the development of APAPinduced liver injury. The immunoexpression of these markers in the liver was examined up to 24 hr post-APAP intraperitoneal in… Show more

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Cited by 5 publications
(3 citation statements)
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“…In fact, secondary antibodies incubation under the same conditions without first antibodies and for the same incubation period in negative controls did not show any staining of liver tissues in our experiment. The results from two recent papers by Sun et al and Zhang et al are in line with us that APAP causes pro-apoptotic mitochondrial Bax translocation. , Similar to our findings, there is no protein expression of Bax in controls evidenced by IHC analysis.…”
supporting
confidence: 93%
“…In fact, secondary antibodies incubation under the same conditions without first antibodies and for the same incubation period in negative controls did not show any staining of liver tissues in our experiment. The results from two recent papers by Sun et al and Zhang et al are in line with us that APAP causes pro-apoptotic mitochondrial Bax translocation. , Similar to our findings, there is no protein expression of Bax in controls evidenced by IHC analysis.…”
supporting
confidence: 93%
“…Another alkylamide-type adduct, PRL, has also been detected in rabbit atherosclerotic plaques ), the liver of an aminoacetophenone-injected rat (Sun et al 2012), and the hippocampus region of the aged rat brain (Hisaka et al, manuscript in preparation) by immunological methods. In particular, HEL is described as one biomarker for oxidative stress (see Sect.…”
Section: Identification Of Prl As An Oxidation Marker For N-3 Fatty Acidmentioning
confidence: 99%
“…In the previous study (Sun et al 2012), the immunoexpression of PUFA oxidation markers PRL, HEL, 4-HHE-histidine and CRA-lysine was examined up to 24 h post-APAP intraperitoneal injection in rats (1 g/kg body weight). Three hr after the postintraperitoneal injection of APAP in rats, vacuolated hepatocytes were observed in the centrilobular region of the hepatic lobule (Fig.…”
Section: Acetaminophen-induced Liver Injurymentioning
confidence: 99%