Immunohistochemical Detection of Potential Microbial Antigens in Granulomas in the Diagnosis of Sarcoidosis

Yamaguchi, Tetsuo; Costabel, Ulrich; McDowell, Andrew; Guzman, Josune; Uchida, Keiji; Ohashi, Kenichi; Eishi, Yoshinobu

doi:10.3390/jcm10050983

Cited by 31 publications

(28 citation statements)

References 80 publications

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“…Additionally, it is worth noting that Propionibacterium acnes 16S rRNA gene has been detected in epithelioid granuloma of PBC livers, but less in adjacent hepatic parenchyma (Harada et al, 2001). Propionibacterium acnes has been suggested as a most likely infectious pathogen of sarcoidosis, a kind of autoimmune disease (Yamaguchi et al, 2021). This study indicated that Propionibacterium acnes may also play a role in PBC which required further investigation.…”

Section: The Microbiome In Primary Biliary Cholangitismentioning

confidence: 78%

The Microbiome in Autoimmune Liver Diseases: Metagenomic and Metabolomic Changes

et al. 2021

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Recent studies have identified the critical role of microbiota in the pathophysiology of autoimmune liver diseases (AILDs), including autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), and primary sclerosing cholangitis (PSC). Metagenomic studies reveal significant decrease of gut bacterial diversity in AILDs. Although profiles of metagenomic vary widely, Veillonella is commonly enriched in AIH, PBC, and PSC. Apart from gut microbiome, the oral and bile microbiome seem to be associated with these diseases as well. The functional analysis of metagenomics suggests that metabolic pathways changed in the gut microbiome of the patients. Microbial metabolites, including short-chain fatty acids (SCFAs) and microbial bile acid metabolites, have been shown to modulate innate immunity, adaptive immunity, and inflammation. Taken together, the evidence of host–microbiome interactions and in-depth mechanistic studies needs further accumulation, which will offer more possibilities to clarify the mechanisms of AILDs and provide potential molecular targets for the prevention and treatment in the future.

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Section: The Microbiome In Primary Biliary Cholangitismentioning

confidence: 78%

The Microbiome in Autoimmune Liver Diseases: Metagenomic and Metabolomic Changes

et al. 2021

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“…Bacterial candidates as infectious causes of sarcoidosis are primarily Mycobacterium tuberculosis and C. acnes (Eishi, 2013). A recent review summarizes current insights in the disease contribution of M. tuberculosis and C. acnes to sarcoidosis (Yamaguchi et al, 2021). Several recent studies have identified C. acnes in granulomas of sarcoidosis patients; detection was achieved with DNA-based techniques, but also with immunohistochemistry/immunofluorescence staining (Nagata et al, 2017;Suzuki et al, 2018;Beijer et al, 2021).…”

Section: Sarcoidosismentioning

confidence: 99%

“…As a possible disease scenario, invasive C. acnes, possibly derived from the skin, could result in a latent, intracellular state, for example in macrophages. Intracellular presence and/or proliferation could activate immune responses, mediated by bacterial factors, followed by the formation of insoluble immune complexes, in particular in individuals predisposed with a Th1 hypersensitivity against C. acnes (Yamaguchi et al, 2021). The multifaceted interactions of C. acnes with the immune system and relevant machineries such as autophagy are presented in the chapter Sensing and Signalling of Extra-and Intracellular C. acnes and Innate and Adaptive Immune Responses.…”

Section: Sarcoidosismentioning

confidence: 99%

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

et al. 2021

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The bacterial species Cutibacterium acnes (formerly known as Propionibacterium acnes) is tightly associated with humans. It is the dominant bacterium in sebaceous regions of the human skin, where it preferentially colonizes the pilosebaceous unit. Multiple strains of C. acnes that belong to phylogenetically distinct types can co-exist. In this review we summarize and discuss the current knowledge of C. acnes regarding bacterial properties and traits that allow host colonization and play major roles in host-bacterium interactions and also regarding the host responses that C. acnes can trigger. These responses can have beneficial or detrimental consequences for the host. In the first part of the review, we highlight and critically review disease associations of C. acnes, in particular acne vulgaris, implant-associated infections and native infections. Here, we also analyse the current evidence for a direct or indirect role of a C. acnes-related dysbiosis in disease development or progression, i.e., reduced C. acnes strain diversity and/or the predominance of a certain phylotype. In the second part of the review, we highlight historical and recent findings demonstrating beneficial aspects of colonization by C. acnes such as colonization resistance, immune system interactions, and oxidant protection, and discuss the molecular mechanisms behind these effects. This new insight led to efforts in skin microbiota manipulation, such as the use of C. acnes strains as probiotic options to treat skin disorders.

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“…Among the potential infectious agents, Mycobacterium tuberculosis and Propionibacterium acnes —currently referred to as Cutibacterium acnes [ 3 ]—are frequently reported to be associated with the pathogenesis of sarcoidosis [ 4 ]. Potential latent infection by both microorganisms, however, has complicated the findings of molecular and immunologic studies [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Detection of Propionibacterium acnes in Granulomas for Differentiating Sarcoidosis from Other Granulomatous Diseases Utilizing an Automated System with a Commercially Available PAB Antibody

et al. 2021

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Propionibacterium acnes is implicated in the pathogenesis of sarcoidosis. We investigated the usefulness of immunohistochemistry (IHC) with a commercially available P. acnes-specific monoclonal antibody (PAB antibody) for differentiating sarcoidosis from other granulomatous diseases. Formalin-fixed paraffin-embedded tissue samples from 94 sarcoidosis patients and 30 control patients with other granulomatous diseases were examined by the original manual IHC method. We also compared the detection frequency of P. acnes in sarcoid granulomas between manual and automated IHC methods. P. acnes was detected in sarcoid granulomas of samples obtained by transbronchial lung biopsy (64%), video-associated thoracic surgery (67%), endobronchial-ultrasound-guided transbronchial-needle aspiration (32%), lymph node biopsy (80%), and skin biopsy (80%) from sarcoidosis patients, but not in any non-sarcoid granulomas of the samples obtained from control patients. P. acnes outside granulomas, however, was frequently detected in both groups. The detection status of P. acnes in granulomas did not correlate with the clinical characteristics of sarcoidosis patients. The automated Leica system exhibited the best detection sensitivity (72%) and almost an identical localization for P. acnes in sarcoid granulomas compared with the manual method. IHC with a PAB antibody is useful for differentiating sarcoidosis from other granulomatous diseases by detecting P. acnes in granulomas. An automated method by the Leica system can be used in pathology laboratories for differential diagnosis of granulomas by IHC with the PAB antibody.

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Immunohistochemical Detection of Potential Microbial Antigens in Granulomas in the Diagnosis of Sarcoidosis

Cited by 31 publications

References 80 publications

The Microbiome in Autoimmune Liver Diseases: Metagenomic and Metabolomic Changes

The Microbiome in Autoimmune Liver Diseases: Metagenomic and Metabolomic Changes

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

Immunohistochemical Detection of Propionibacterium acnes in Granulomas for Differentiating Sarcoidosis from Other Granulomatous Diseases Utilizing an Automated System with a Commercially Available PAB Antibody

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