Immunohistochemical detection of progesterone receptor in formalin-fixed and paraffin-embedded breast cancer tissue using a monoclonal antibody

Scharl, A.; Vierbuchen, M.; Conradt, B; Moll, W; Würz, H.; Bolte, A.

doi:10.1007/bf02390663

Cited by 26 publications

(17 citation statements)

References 12 publications

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“…Then, immunohistochemistry (IHC) staining was performed in 85 different-grade chondrosarcoma specimens to assess the expression level of TCF-1. A total of 25 % of tumor samples were positive [23] for TCF-1 staining. The results showed that TCF-1 expression level was higher in DDCS than in CCS, especially in the dedifferentiated portion of DDCS.…”

Section: Resultsmentioning

confidence: 99%

TCF-1 participates in the occurrence of dedifferentiated chondrosarcoma

Tang

Guo

et al. 2016

Tumor Biol.

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The present study demonstrated that T cell factor 1 (TCF-1) protein, a component of the canonical Wnt/β-catenin signaling pathway, can regulate the expression of runt-related transcription factor 2 (runx2) gene and Sry-related HMG box 9 (sox9) gene, which may participate in the differentiation of chondrosarcoma. Dedifferentiated chondrosarcoma (DDCS) is a special variant of conventional chondrosarcoma (CCS), associated with poor survival and high metastasis rate. However, little is known about the mechanism of its occurrence; thus, no effective treatment is available except surgery. Earlier, high expression of runx2 and low expression of sox9 were found in DDCS compared with CCS. Using Western blot to detect clinical tissue samples (including 8 CCS samples and 8 DDCS samples) and immunohistochemistry to detect 85 different-grade chondrosarcoma specimens, a high expression of TCF-1 in DDCS tissues was found compared with CCS tissues. This difference in expression was related to patients’ prognosis. Results of luciferase, chromatin immunoprecipitation, and gel electrophoresis mobility shift assays demonstrated that TCF-1 protein could bind to the promoter of runx2 gene directly and sox9 gene indirectly. Hence, it could regulate expression of runx2 gene positively and sox9 gene negatively. Furthermore, in vitro and in vivo experiments showed that TCF-1 protein was closely related to the phenotype and aggressiveness of chondrosarcoma. In conclusion, this study proved that TCF-1 participates in the dedifferentiation of DDCS, which may be mediated by runx2 gene and sox9 gene. Also, TCF-1 can be of important prognostic value and a promising therapeutic target for DDCS patients.

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Section: Resultsmentioning

confidence: 99%

TCF-1 participates in the occurrence of dedifferentiated chondrosarcoma

Tang

Guo

et al. 2016

Tumor Biol.

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“…The sections were probed with primary antibodies through the night at 4°C and the signal was developed with EnVision detection kit from DAKO. Immunostaining labeling intensities were defined as the number and the staining degree of positive cells:+less than 10% of the cells were positive and positive weakly; ++10%–50% of the cells were positive and positive medium, +++50–80% of the cells were positive and strong positive, ++++more than 80% cells were positive[34].The primary antibodies used in this study include anti-glypican-3(1∶500,ABBIOTEC),anti-MT(1∶50,DAKO), anti-Nrf2(1∶100,Abcam)…”

Section: Methodsmentioning

confidence: 99%

Maotai Ameliorates Diethylnitrosamine-Initiated Hepatocellular Carcinoma Formation in Mice

Yi¹,

Long²,

Yang

et al. 2014

PLoS ONE

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Consumption of alcohol is closely related to liver disease, such as hepatic fibrosis or even hepatocellular carcinoma (HCC). However, epidemiological and experimental studies indicated that consumption of Maotai, one of the famous liquors in China, exhibits no significant correlation with hepatic fibrosis or cirrhosis as other beverage sources do. This study detected the relationship of Maotai consumption and HCC development in a diethylnitrosamine (DEN)-initiated HCC animal model. DEN was given to mice at a dose of 100 mg/kg, ip, and 50 mg/kg, ip in the following week. Mice were simultaneously given Maotai or an equal amount of ethanol (53%, 5 ml/kg/day, 5days/week for up to 35weeks). At 3-week and 35- week of the experiment, serum and livers were collected for biochemical and histopathological examination of liver injury and incidence of HCC. Real-time RT-PCR, immunohistochemistry and Western blotting were used to examine the expression of metallothionein-1/2 (MT-1/2), NF-E2-related factor 2 (Nrf2), glutamate-cysteine ligase catalytic subunit (GCLC) and modified subunit (GCLM). We identified tissue damage and dysfunction of liver in ethanol + DEN-treated mice, whereas the extent of injury was reduced in Maotai+ DEN –treated mice. Significant Glypican-3(GPC3) expression and precancerous injury or HCC were seen in approximately 50% of mice with ethanol+ DEN, but barely be seen in Maotai + DEN-treated mice. A higher expression of MT-1/2, Nrf2 and GCLC could be seen in Maotai + DEN-treated mice. Thus, Maotai liquor ameliorates the formation of DEN-induced HCC in mice, and the protection mechanism is possibly related with the activation of anti-oxidation factors, such as MTs, Nrf2 and GCLC.

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“…Immunohistochemical staining of cells for β-catenin and AXIN2 was scored according to stain intensity and extent of cells stained by semi-quantitative evaluation [16]. Brown-yellow staining observed mainly in cell plasma and cell nucleus was considered positive for β-catenin and AXIN2 protein expression.…”

Section: Methodsmentioning

confidence: 99%

Expression of β-catenin and AXIN2 in ameloblastomas

Wei

Zhong

Guo

et al. 2013

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Aim of the studyTo investigate the expression status and association of β-catenin and AXIN2 in ameloblastoma.Material and methods30 ameloblastoma specimens and 10 normal oral mucosa tissues were enrolled in the study. The protein and RNA levels of β-catenin and AXIN2 were detected by immunohistochemistry staining, Western blot, and real-time PCR analysis. The relationship between β-catenin and AXIN2 protein and clinico-pathological parameters and prognosis was subsequently determined.ResultsThe results show that β-catenin mRNA expression was 1.24-fold higher in ameloblastomas than that in normal mucosa tissues, but AXIN2 mRNA expression was 0.47-fold lower in ameloblastomas than that in normal mucosa tissues (p < 0.05). The Western blot results show that β-catenin and AXIN2 protein in ameloblastomas had a significantly higher level than normal mucosa tissues (p < 0.05). Immunohistochemical staining of β-catenin and AXIN2 protein in ameloblastoma was expressed at a higher level than normal oral mucosa (p < 0.05).ConclusionsAXIN2 and β-catenin play an important role in the tumorigenesis and progression of ameloblastoma.

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Immunohistochemical detection of progesterone receptor in formalin-fixed and paraffin-embedded breast cancer tissue using a monoclonal antibody

Cited by 26 publications

References 12 publications

TCF-1 participates in the occurrence of dedifferentiated chondrosarcoma

TCF-1 participates in the occurrence of dedifferentiated chondrosarcoma

Maotai Ameliorates Diethylnitrosamine-Initiated Hepatocellular Carcinoma Formation in Mice

Expression of β-catenin and AXIN2 in ameloblastomas

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