Immunohistochemical Detection of S-100, S-1OOα, S-100β Proteins, Glial Fibrillary Acidic Protein, and Neuron Specific Enolase in the Prenatal and Adult Human Salivary Glands

Lee, Suk Keun; Kim, EunCheol; Chi, Je-G.; Hashimura, Kouji; Mori, Masahiko

doi:10.1016/s0344-0338(11)80677-1

Cited by 22 publications

(17 citation statements)

References 18 publications

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“…These results are contrary to those of Haimoto et al (1987) who reported an inverse distribution in human glands, but confirmed observations of Jayasinghe et al (1993) and Molin et al (1984) who investigated rat salivary glands. Mori et al (1990) and Lee et al (1993) described S-100α-and βpositive serous cells in human major salivary glands but found the same distribution pattern of S-100 in the duct system as we did in bovine material. This confined localization of S-100α tempts one to speculate that the protein takes part in transport mechanisms of striated duct cells.…”

Section: Discussionsupporting

confidence: 81%

“…They found S-100α mainly in serous acini, whereas S-100β was observed in excretory duct epithelium and in myoepithelial cells. In contrast, Lee et al (1993) observed both subunits in secretory cells of salivary glands, the βsubunit in intercalated duct epithelium, and S-100α in the excretory ducts. Results of Mori et al (1990) and Nakazato et al (1985) supported these findings and confirmed that mucous cells do not react for S-100.…”

Section: Introductionmentioning

confidence: 71%

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Distribution of S-100 protein and its subunits in bovine exocrine glands

Lauboeck

Egerbacher²

1997

Histochemistry and Cell Biology

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The distribution of S-100 protein and its alpha- and beta-subunits in bovine exocrine glands was studied by indirect immunohistochemistry. The entire spectrum of salivary glands, glands of the respiratory tract, intestinal glands, male and female genital glands, and skin glands was examined. S-100 and its beta-subunit were identified in most serous secretory cells of mixed salivary glands, although secretory acini in some serous glands remained unreactive for these antigens. Mucous cells were constantly negative; mucoid cells were positive in the lacrimal and Harderian gland. The alpha-subunit of S-100 protein was identified in serous cells but the staining reaction was faint. Subunits of S-100 showed a characteristic distribution along the excretory duct systems of compound glands: S-100 and the beta-subunit were present in intercalated duct epithelium, while striated duct epithelium stained for S100-alpha. Therefore, it is suggested that S100-alpha is related to resorption and secretion in striated ducts, while S100-beta may govern acinar exocytosis and probably regulates proliferation and differentiation of glandular cells. Differing staining intensities for S-100 and its subunits in secretory cells of exocrine glands most probably indicate functional differences with regard to secretory activity and the cell cycle.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 71%

Distribution of S-100 protein and its subunits in bovine exocrine glands

Lauboeck

Egerbacher²

1997

Histochemistry and Cell Biology

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“…Heterogeneity of salivary glands is poorly understood. Lee et al [2] described the details of stage-dependent changes in immunoreactivity toward S100, GFAP, and NSE by using 100 human fetal salivary glands; however, they did not discriminate among the gland types, and therefore, their results probably reflect type- and not stage-specific differences.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, previously reported data on expression of neuronal markers in fetal salivary glands are inconsistent; for example, Gustafsson et al [1] and Lee et al [2] stated that fetal parotid glands expressed GFAP, whereas Chi [19] and Ianez et al [14] found no evidence for GFAP immunostaining in these glands at a similar gestational age. Therefore, in this study, we aimed to comprehensively examine the immunohistochemical reactivity toward the described markers not only in the major parotid and submandibular glands, but also in the minor glands such as pharyngeal gland near the palatine tonsil, the lingual gland at the dorsum of the tongue, and the palatal gland by using specimens from cadavers of elderly persons.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of glandular cells in the human salivary glands: an immunohistochemical study using elderly adult and fetal specimens

et al. 2013

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Using immunohistochemical staining for alpha-smooth muscle actin (α-SMA), glial fibrillary acidic protein (GFAP), S100 protein (S100), p63, cytokeratin 14 (CK14), and cytokeratin 19 (CK19), we studied acinar and myoepithelial cells of major and minor salivary glands obtained from 14 donated cadavers (78-92 years old) and 5 donated fetuses (aborted at 15-16 weeks of gestation). CK and p63 expression was investigated only in the adult specimens. SMA was detected in all adult glands as well as in fetal sublingual and pharyngeal glands. GFAP expression was seen in a limited number of cells in adult glands, but was highly expressed in fetal pharyngeal glands. S100-positive myoepithelial-like cells were present in adult minor glands as well as in fetal sublingual and pharyngeal glands. Expression of p63 was evident in the ducts of adult glands. CK14 immunoreactivity was observed in a limited number of glandular cells in adults, in contrast to consistent expression of CK19. In both adults and fetuses, a mosaic expression pattern was usually evident for each of the examined proteins. A difference in immunoreactivity for the nerve markers GFAP and S100 was observed between the major and minor glands. Thus, in the present histologic study, we distinguished between the specific gland types on the basis of their immunohistochemical staining. A mosaic expression pattern suggested that the immunoreactivity against nerve protein markers in myoepithelial cells could not be due to the persistence of neural crest remnants or the physiological status of the gland, such as age-related degeneration.

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“…Data are shown as median and interquartile ranges, 5th-95th centile. Studies of human fetuses have shown that members of the S100 protein family are present in different tissues of the salivary glands during ontogenesis: this holds especially for S100A, S100A1, and S100A9, while S100B is known to be essentially absent from fetal salivary glands from 32 weeks of gestation onwards and in adulthood [15,16].…”

Section: Discussionmentioning

confidence: 99%