1997
DOI: 10.1182/blood.v90.4.1664
|View full text |Cite
|
Sign up to set email alerts
|

Immunohistochemical Detection of the Epstein-Barr Virus–Encoded Latent Membrane Protein 2A in Hodgkin's Disease and Infectious Mononucleosis

Abstract: We describe two new monoclonal antibodies specific for the Epstein-Barr virus (EBV)-encoded latent membrane protein 2A (LMP2A) that are suitable for the immunohistochemical analysis of routinely processed paraffin sections. These antibodies were applied to the immunohistochemical detection of LMP2A in Hodgkin's disease (HD). LMP2A-specific membrane staining was seen in the Hodgkin and Reed-Sternberg (HRS) cells of 22 of 42 (52%) EBV-positive HD cases, but not in 39 EBV-negative HD cases. In lymphoid tissues fr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
32
0

Year Published

2001
2001
2008
2008

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 117 publications
(33 citation statements)
references
References 54 publications
1
32
0
Order By: Relevance
“…In primary EBV infection the infected cells follow a productive cycle and viral replication can be demonstrated, whereas in EBV-associated tumors the virus within the infected cells is found in a status of restricted latency (8). In HL the detection of EBER, LMP-1 and EBNA-1 in the absence of EBNA-2 is consistent with a type II latency of EBV (14). Viral replication could be demonstrated by the detection of ZEBRA protein (15).…”
Section: Discussionsupporting
confidence: 64%
“…In primary EBV infection the infected cells follow a productive cycle and viral replication can be demonstrated, whereas in EBV-associated tumors the virus within the infected cells is found in a status of restricted latency (8). In HL the detection of EBER, LMP-1 and EBNA-1 in the absence of EBNA-2 is consistent with a type II latency of EBV (14). Viral replication could be demonstrated by the detection of ZEBRA protein (15).…”
Section: Discussionsupporting
confidence: 64%
“…This gene expression pattern is observed in lymphoproliferative disease occurring in the context of impaired cell mediated immunity, such as PTLD (Young et al, 1989). In contrast, in HL, the expression of viral proteins is restricted to EBNA1, LMP1, LMP2A and 2B, the latency II pattern of gene expression or default programme (Wu et al, 1990;Pallesen et al, 1991;Rowe et al, 1992;Deacon et al, 1993;Grasser et al, 1994;Niedobitek et al, 1997;Thorley-Lawson & Gross, 2004).…”
mentioning
confidence: 97%
“…6 Defined by in situ analysis, the EBV expression pattern in the HR/S cells is type II; they express Epstein-Barr virus early RNA (EBER), EBNA-1 and LMPs. 7,8 LMP-1 and LMP-2A are believed to provide survival signals to B lymphocytes by mimicking the externally activated CD40 and BCR pathways, respectively. 9 In the EBV-positive cases, expression of these viral proteins could be responsible for the survival of surface Ig-negative germinal-center B cells, the putative precursors of H/RS cells.…”
mentioning
confidence: 99%