Immunohistochemical determination of estrogen receptor-α in canine vaginal biopsies throughout proestrus, estrus, and early diestrus

Ithurralde, Javier; Costas, Ana Laura; Pessina, Paula; Cueto, Enrique; Fila, Danilo; Meikle, A.

doi:10.1016/j.theriogenology.2013.07.007

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…All of the following methods were previously validated for dogs (Ravivarapu et al 2000;Gallelli et al 2010;Cabrera Blatter et al 2011;Ithurralde et al 2013).…”

Section: Animalsmentioning

confidence: 99%

Plasma ACTH, α-MSH and cortisol variations in the dog during the oestrous cycle in different photoperiods

Gallelli¹,

Monachesi²,

Miceli³

et al. 2015

Vet. Med. - Czech

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ABSTRACT:The hypothalamic-pituitary-adrenal axis (HPA) is a complex system regulated by multiple factors. Sexual dimorphism of this axis has been described in different species under physiological conditions and it has been proposed that sexual hormones could have an effect on it. There are only a few reports about sex-linked variations in HPA axis hormones in the dog. Thus, studying the impact of sexual hormones on the HPA axis would broaden the knowledge about its function in this species. Therefore, the objective of this study was to determine whether there are variations in HPA plasma hormones (ACTH, alfa-melanocyte-stimulating hormone [α-MSH] and cortisol) according to the sex and photoperiod (positive or negative photoperiod were considered when the duration of the light hours of the day was more than 12 or less than 12, respectively) under basal conditions (like anoestrus) and throughout the oestrous cycle in the female dog. The population under study consisted of 11 intact female and 14 intact male dogs. Under basal conditions neither ACTH nor α-MSH concentrations showed differences between sexes and different photoperiods. Cortisol showed greater values in the negative photoperiod than in the positive, both in females and males (P = 0.03 and P = 0.015, respectively). Throughout the oestrous cycle, all the studied hormones showed variations (P < 0.0001). The greatest concentrations of ACTH were observed at proestrus, while α-MSH and cortisol showed their greatest concentrations at oestrus. The three hormones decreased in diestrus. ACTH and cortisol concentrations were higher in the negative photoperiod (P = 0.04 and P < 0.0001, respectively), while α-MSH concentrations were higher in the positive photoperiod (P = 0.012). In the group of females oestradiol and progesterone correlated with ACTH (r = 0.75, P < 0.0001; r = 0.34, P < 0.01, respectively), α-MSH (r = 0.49, P < 0.0001; r = 0.52, P < 0.0001, respectively) and cortisol (r = 0.33, P < 0.01; r = 0.5, P < 0.0001, respectively). These results show that in females, HPA axis hormones vary during the oestrous cycle in relation to oestradiol and progesterone fluctuations. The ACTH, α-MSH and cortisol concentrations also showed differences between photoperiods in females, but only cortisol did so in males. These findings suggest that sexual hormones could have an effect on the HPA axis. Further research needs to be done to fully understand this interaction and the mechanisms involved.

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“…All of the following methods were previously validated for dogs (Ravivarapu et al 2000;Gallelli et al 2010;Cabrera Blatter et al 2011;Ithurralde et al 2013).…”

Section: Animalsmentioning

confidence: 99%

Plasma ACTH, α-MSH and cortisol variations in the dog during the oestrous cycle in different photoperiods

Gallelli¹,

Monachesi²,

Miceli³

et al. 2015

Vet. Med. - Czech

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“…It was reported that VFP occurs due to a strong response of vaginal mucosa to the estrogens during pro-estrous and early estrus (Sontas et al, 2010;Alan et al, 2007). In healthy bitches, reduction of ER expression in vaginal tissue was detected from proestrus and late estrus (Ithurralde et al, 2013). de Brito et al (2006) compared ER-a in the vaginal epithelium of bitches with transmissible venereal tumor (TVT) or control bitches, and they indicated that there was a close relationship between neoplastic cells and cells of the vaginal stroma, which were highly positive for ER-a.…”

Section: Discussionmentioning

confidence: 98%

Determination of estrogen receptor, progesterone receptor and epidermal growth factor receptor in canine vaginal tumors and vaginal fold prolapse: A preliminary study

Uçmak

Ateş²,

Bamaç

et al. 2022

Journal of Istanbul Veterinary Sciences

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Eight dogs with a vaginal mass were incorporated into the study. According to the vaginal examination (vaginoscopy, exfoliative vaginal cytology) and histopathology, the groups were designed as vaginal fold prolapse (Group VFP; n=3) and vaginal tumor (Group VT; n=5). Vaginal tissue samples were homogenized. Tissue homogenates were analyzed with enzyme-linked immunosorbent assay (ELISA) to determine the levels of estrogen receptor (ER), progesterone receptor (PR), and epidermal growth factor receptor (EGFR). The mean ER level was significantly higher in group VT compared to the group VFP (P<0.05). However, PR and EGFR levels did not showed significance related to the groups (P>0.05). In conclusion, significant differences were obtained between the bitches with VT and VFP related to ER levels although both groups have pathological conditions. Further studies are needed to discuss these pathologies with healthy bitches in terms of steroid receptor and EGFR levels in both vaginal tissue and blood sera.

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“…Non-specific binding sites were blocked with normal horse serum for 30 min (Vectastain ABC-Kit, Elite, PK-6102, Burlingame, CA, USA). Sections were incubated in a humidified chamber for 2 h with either a 1:100 PBS:anti-MC2R primary antibody dilution (rabbit polyclonal; sc-13107, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or with a 1:25 dilution of the anti-ERα primary antibody (mouse monoclonal, sc-787, Santa Cruz Biotechnology, Santa Cruz, CA, USA) in PBS (Ithurralde et al, 2013). After being washed with PBS, sections were incubated for 1 h with a 1:200 dilution of the biotinylated anti-rabbit antibody (BA-1000, Vector, Burlingame, CA, USA) or the biotinylated anti-mouse antibody (BA-200, Vector, Burlingame, CA, USA) as appropriate for each section based on the previously applied primary antibody.…”

Section: Immunostaining For Erα and Mc2r In Adrenal Glandsmentioning

confidence: 99%

Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation

Gallelli¹,

Lombardo

Vissio

et al. 2016

Research in Veterinary Science

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Immunohistochemical determination of estrogen receptor-α in canine vaginal biopsies throughout proestrus, estrus, and early diestrus

Cited by 4 publications

References 23 publications

Plasma ACTH, α-MSH and cortisol variations in the dog during the oestrous cycle in different photoperiods

Plasma ACTH, α-MSH and cortisol variations in the dog during the oestrous cycle in different photoperiods

Determination of estrogen receptor, progesterone receptor and epidermal growth factor receptor in canine vaginal tumors and vaginal fold prolapse: A preliminary study

Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: Sex-linked and seasonal variation

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