Immunohistochemical distinction of high-grade mucoepidermoid carcinoma and epidermoid carcinoma of the parotid region

Sobral, Ana Paula Veras; Loducca, S. V. L.; Kowalski, Luiz Paulo; Santos, Izandro R B; Almeida, Orlando José de; Araújo, Ney Soares de; Araújo, Vera Cavalcanti de

doi:10.1016/s1368-8375(01)00089-6

Cited by 26 publications

(30 citation statements)

References 8 publications

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“…In general, CK7 was reported to be expressed by the vast majority of studied salivary gland tumors, including benign and malignant tumors. 19,[22][23][24][26][27][28][29][30][31] These results are in accordance with our findings showing that all cases of malignant salivary gland tumors were positive for CK7, despite differences in intensity, positivity, and distribution of staining. Regarding the latter, the preferential CK7 immunohistochemical expression in certain subtypes of tumor cells observed in our study, such as luminal over myoepithelial cells in adenoid cystic carcinomas, agrees with the findings of other authors and partially reiterates the distribution of CK7 expression in the different cellular compartments of normal salivary glands.…”

Section: Discussionsupporting

confidence: 92%

“…Although several investigators have examined the expression of CKs in neoplastic lesions of salivary glands, 19,[21][22][23][24][25][26][27][28][29][30][31] to the best of our knowledge this is the first time that the combined CK7 and CK20 profile of malignant salivary gland tumors is analyzed in a sizeable group of these tumors.…”

Section: Discussionmentioning

confidence: 99%

“…Our results in squamous cell carcinomas secondarily involving salivary glands largely confirm these previous studies, in that most tumors are negative for both CK7 and CK20, the rest being weakly focally positive only for CK7. Given the relative frequency of squamous cell carcinomas involving major and minor salivary glands, as a result of metastasis or direct extension from adjacent sites, and the possibility of confusion between squamous cell carcinomas and malignant salivary gland tumors, especially high-grade mucoepidermoid carcinomas, 19 the differential CK7/CK20 immunoprofile of these tumors can facilitate their discrimination. In this regard, the negative or focally and weakly positive CK7 staining in squamous cell carcinomas contrasts with the uniformly strong and predominantly diffuse CK7 immunoreactivity of mucoepidermoid carcinomas, which persists among high-grade tumors.…”

Section: Discussionmentioning

confidence: 99%

“…17 Moreover, diagnostic difficulties arise when carcinomas from remote sites metastasize to salivary glands 18 or when squamous cell carcinomas of mucosal or skin origin metastasize to or invade the salivary glands. 19 Finally, the overlapping histopathologic features of the numerous types of malignant salivary gland tumors often pose difficulties in the accurate classification of these neoplasms, which may exhibit a wide range of biologic behaviors. 20 In all these instances, assessment of the CK profile could facilitate the precise diagnosis of malignant salivary gland tumors.…”

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confidence: 99%

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Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

et al. 2004

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On the basis of the heterogeneity of cytokeratins 7 and 20 expression in malignant epithelial tumors, the cytokeratin 7/20 immunophenotype has served as a useful diagnostic tool for discrimination of primary and/or metastatic carcinomas of unknown origin. However, the expression pattern of these cytokeratins in malignant salivary gland tumors has not been thoroughly studied. Our study material was composed of 84 malignant tumors of primary major or minor salivary gland origin. Nine histologic types of carcinoma were represented, including mucoepidermoid (26 cases), adenoid cystic (25), polymorphous low grade (11), salivary duct (8), acinic cell (4), ex mixed tumor (3), not otherwise specified (3), clear cell (2), and basal cell (2). In all, 13 cases of primary skin or mucosal squamous cell carcinoma with secondary salivary gland involvement were also examined. Immunoreactivity for cytokeratin 7 was evident in all malignant salivary gland tumors; the staining pattern was diffuse and strong in 62 cases, and focal and strong in 22 cases. In contrast, 78 cases were negative for cytokeratin 20, whereas only six cases (two mucoepidermoid, one adenoid cystic, and three salivary duct) displayed focal weak positivity. Overall, 92.9% of malignant salivary gland tumors were characterized by a cytokeratin 7 positive / 20 negative immunoprofile, the remaining 7.1% of cases being positive for both cytokeratins. The latter phenotype was more common in salivary duct carcinomas (Pr0.05). On the other hand, most squamous cell carcinomas (69%) were negative for both cytokeratins, while the remaining cases (31%) were negative for cytokeratin 20 and focally weakly positive for cytokeratin 7. We suggest that assessment of cytokeratin 7/20 immunoprofile may facilitate the differential diagnosis of (a) primary malignant salivary gland tumors from metastatic tumors, (b) metastatic salivary gland tumors, (c) primary salivary gland tumors, especially mucoepidermoid carcinomas, from squamous cell carcinomas, and (d) salivary duct carcinomas from other malignant salivary gland tumors.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

et al. 2004

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“…Based on previous studies on p27/ skp-2 expression and oral (mucosal) squamous cell carcinoma, it may be concluded that the histogenesis of PSCCA is more closely related to mucosal SCCA rather than the salivary duct apparatus [25]. This information may provide us with an additional diagnostic tool for distinguishing between high grade MEC and PSCCA [35].…”

Section: Pscca and Mstmentioning

confidence: 89%

P27/SKP-2 Histochemical Profile is Relevant to Malignant Salivary Gland Tumors (MST) Histogenesis and Tumor Grade

Akrish

Ben‐Izhak

Peled

2011

Head and Neck Pathol

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Malignant salivary gland tumors (MST) represent over more than 24 distinct morphological subtypes. Most high grade tumors arise from the excretory duct portion of the salivary gland apparatus; the remainder from the intercalated duct portion. Altered p27/skp-2 expression has been associated with tumor aggressiveness and histologic differentiation. In our study, we analyzed p27/skp-2 expression proteins on series of malignant salivary gland tumors in order to assess their value as a histogenetic marker, which is relevant to tumor grade. 61 MST cases were segregated by proposed histogenesis and immunohistochemistry was performed using antibodies directed against p27 and skp-2. MST of proposed intercalated duct origin (n = 27) showed strong p27 expression (n = 25/27; 93%) in the vast majority of cases and all cases weakly expressed skp-2. MST of proposed excretory duct origin (n = 32) showed strong p27 expression (n = 18/32; 56%) and moderately strong/ strong skp-2 expression (n = 18/32; 56%), respectively, in over half the cases. MST of intercalated duct origin showed evident p27/skp-2 inverse correlation. Differences in p27/skp-2 expression among the MST subtypes correlated with histogenesis and tumor grade, which reinforces the notion that tumor behavior is relevant to the portion of the salivary gland unit from which they arise. MST of proposed intercalated duct origin strongly expressed p27, and not skp-2, unlike MST of proposed excretory duct origin. The immunohistochemical profile of high grade mucoepidermoid carcinoma was distinct from its low/intermediate grade counterparts, suggesting a separate identity. These results may influence future decision making when formulating workable MST categorization schemes. Further studies on a larger series of MST are warranted in order to support the value of our findings.

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Salivary Gland and Other Head and Neck Structures

Schuerch

2011

Handbook of Practical Immunohistochemistry

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Immunohistochemical distinction of high-grade mucoepidermoid carcinoma and epidermoid carcinoma of the parotid region

Cited by 26 publications

References 8 publications

Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors

P27/SKP-2 Histochemical Profile is Relevant to Malignant Salivary Gland Tumors (MST) Histogenesis and Tumor Grade

Salivary Gland and Other Head and Neck Structures

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