Immunohistochemical Evaluation of Angiogenesis and Tryptase-positive Mast Cell Infiltration in Periapical Lesions

Lima, Sílvio Carlos Albuquerque; Toral-Rizo, Víctor Hugo; Silva‐Sousa, Yara Teresinha Corrêa; Almeida, Luciana Yamamoto; Almeida, Oslei Paes de; León, Jorge Esquiche

doi:10.1016/j.joen.2011.08.024

Cited by 23 publications

(29 citation statements)

References 33 publications

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“…Recent studies have focused on the etiopathogenesis of chronic periapical lesions, odontogenic cysts and tumors, using immunohistochemistry to investigate the role of cytokines, growth factors, cellular differentiation factors, tumor suppressor genes and oncogenes in the initiation, development, and progression of these lesions (4,6,(12)(13)(14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors

et al. 2012

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The aim of this study was to assess the immunohistochemical expression of p63 protein, epidermal growth factor receptor (EGFR) and Notch-1 in the epithelial lining of radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). For this study, 35 RC, 22 DC and 17 KOT were used. The clinical and epidemiological data were collected from the patient charts filed in the Oral Pathology Laboratory, University of Ribeirão Preto, Brazil. Immunohistochemical reactions against the p63, EGFR and Notch-1 were performed in 3-µm-thick histological sections. The slides were evaluated according to the following criteria: negative: <5% of positive cells, low expression: 5-50% of positive cells, and high expression: >50% of positive cells. Moreover, the intensity of EGFR and Notch-1 expressions was also evaluated. Fisher's exact test and Spearman's correlation coefficients were used for statistical analysis, considering a significance level of 5%. Almost all cases demonstrated p63, EGFR and Notch-1 expressions. The p63 expression was significantly higher in KOT (p<0.001). Positive correlation between these immunomarkers was observed. These findings suggest the participation of p63, EGFR and Notch-1 in the development, maintenance and integrity of cystic odontogenic epithelial lining, favoring lesion persistence. The high expression of p63 in KOT suggests that it may be related to their more aggressive biological behavior and marked tendency to recurrence.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors

et al. 2012

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“…MVD and microvessel area (MVA), assessed through CD34 and CD105 expression, as well as lymphatic MVD (LMVD) and MVA (LMVA), assessed through D2-40 expression, were established identifying the most vascularized areas within the lesions ("hot spots"), which were chosen at low magnification (100x) and subsequently photographed in fve high-power fields (at 400x magnifcation) 14 . The images were made using the Aperio Scan Scope ® software, which depicted the image size (height: 580 µm/width: 320 µm), resulting in a total area of 185,600 µm 2 .…”

Section: Methodsmentioning

confidence: 99%

FASN expression, angiogenesis and lymphangiogenesis in central and peripheral giant cell lesions

et al. 2014

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Central giant cell lesion (CGCL) and peripheral giant cell lesion (PGCL) are non-neoplastic proliferative processes of the jaws. PGCL is a reactive process induced by irritant local factors and CGCL is an intra-osseous lesion of unknown etiology. Both lesions exhibit similar histologic features showing abundant mononuclear cells, admixed with a large number of multinucleated giant cells and a rich vascularized stroma with extravasated erythrocytes, hemosiderin deposition, and blood-filled pools. Recent studies have linked fatty acid synthase (FASN) with angiogenesis.Objective To evaluate angiogenesis and lymphangiogenesis and their relationship with FASN expression in CGCL and PGCL. Material and Methods Thirteen CGCL and 14 PGCL of the jaws were selected for immunoexpression of FASN; CD34 and CD105 (to assess blood microvessel density [MVD] and microvessel area [MVA]); and D2-40 (to assess lymphatic MVD and MVA). Results Within PGCL and CGCL, MVD-CD34 was signifcantly higher than MVD-CD10S, followed by MVD-D2-40. Moreover, a signifcantly higher number of FASN-positive multinucleated giant cells than mononuclear cells were observed. Between PGCL and CGCL, only MVD-CD34 and all MVA were signifcantly higher in PGCL. Positive correlation between MVA-CD10S with FASNpositive mononuclear cells in both lesions was observed. Conclusions Our results show both lesions exhibiting similar levels of FASN expression and neoangiogenesis, suggesting constitutive processes that regulate tissue maintenance.

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“…The major component present in the secretory granules of MC is tryptase, an enzyme released along with other mediators in the extracellular matrix after the activation/degranulation of MC. 23,24 It has been suggested that tryptase stimulates the proliferation of fibroblasts and synthesis of collagen, and that it is involved in fibrinogenesis. 23,25 In addition, tryptase plays a role in other biological activities, such as: the stimulation of the proliferation of smooth muscle cells and bronchial epithelial cells, the degradation of vasoactive intestinal peptide, and angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Mast cells produce and release proteolytic enzymes, tryptase and chymase, which mediate the migration of endothelial cells and the release of angiogenic factors, such as vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (b-FGF), transforming growth factor-beta (TGF-b), tumor necrosis factor-alpha (TNF-α), and interleukin (IL)-8, creating a microenvironment that favors neoplastic development. 24,34 …”

Section: Discussionmentioning

confidence: 99%

Histochemical and immunohistochemical differences between solitary oral fibroma and fibrous papule of the face

Henriques

Freitas

Pires³

et al. 2016

An. Bras. Dermatol.

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BackgroundThe morphological similarities between fibrous papules of the face and multiple sporadic oral fibromas were mentioned long ago and a relationship between them has been reported in the literature.ObjectiveThe aim of this study was to evaluate the participation of mast cells, elastin and collagen in a series of oral fibromas and fibrous papules of the face in order to better understand the possible role of these factors in fibrosis and the formation of these lesions.MethodsThirty cases of oral fibroma involving the buccal mucosa and 30 cases of fibrous papules of the face were selected. Tissue samples were submitted to picrosirius red staining and immunohistochemistry using anti-elastin and anti-tryptase antibodies.ResultsThe percentage of tryptase-positive mast cells and expression of elastin were higher in cases of fibrous papules of the face (p < 0.05). In contrast, a higher intensity of collagen deposition was observed in oral fibromas. The results showed mast cell accumulation and higher elastin synthesis in fibrous papules of the face, and mast cell accumulation with higher collagen fiber synthesis in oral fibromas.ConclusionThese findings support the hypothesis that mast cells influence the development and growth of these lesions through different mechanisms.

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Immunohistochemical Evaluation of Angiogenesis and Tryptase-positive Mast Cell Infiltration in Periapical Lesions

Cited by 23 publications

References 33 publications

Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors

Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors

FASN expression, angiogenesis and lymphangiogenesis in central and peripheral giant cell lesions

Histochemical and immunohistochemical differences between solitary oral fibroma and fibrous papule of the face

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