Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Yao, Wenming; Godfrey, Donald A.

doi:10.1002/(sici)1097-0029(19980501)41:3<270::aid-jemt10>3.0.co;2-l

Cited by 40 publications

(36 citation statements)

References 41 publications

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“…The following SOC nuclei are labeled: lateral superior olive (LSO), dorsal periolivary (DPO) region, medial superior olive (MSO), ventral nucleus of the trapezoid body (VNTB), medial nucleus of the trapezoid body (MNTB), and superior paraolivary nucleus (SPN). Dorsal (D) and lateral (L) directions are as indicated From P7 through adult ages, cholinergic-like cells (as defined by either ChAT immunocytochemistry or AChE histochemistry) show comparable labeling distributions in the SOC as described in other adult rodents (Osen et al 1984;Altschuler et al 1985;Vetter et al 1991;Yao and Godfrey 1998). In this study, our observations are limited to only the darkest-staining cells that met strict criteria (see Materials and methods).…”

Section: General Observationsmentioning

confidence: 75%

Differential development of cholinergic-like neurons in the superior olive: a light microscopic study

Simmons¹,

Bertolotto²,

Typpo³

et al. 1999

Anatomy and Embryology

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To better understand the development of cholinergic-like neurons within the superior olivary complex, we investigated the onset and distribution of two well-known markers of cholinergic-like neurons in hamsters: choline acetyltransferase (ChAT) and acetylcholinesterase (AChE). From embryonic day (E) 14 through postnatal day (P) 0, olivary cells immunopositive for ChAT were restricted to the rostral periolivary (RPO) area. Between P0 and P3, ChAT-positive cells are found in progressively more caudal and ventral periolivary locations. Although rostral and ventral periolivary cells exhibited an early onset of ChAT expression, stable numbers were not reached until P4. In contrast, ChAT expression within the lateral superior olive (LSO) is not visible until after P0 and higher numbers of ChAT-positive cells are obtained by P5. The AChE expression lags several days but follows roughly the same pattern of onset as for ChAT. Additionally in rostral and ventral periolivary regions as well as in the LSO, there were fewer AChE-labeled cells than ChAT-labeled cells. The observed temporal relationships in cholinergic-like expression within olivary cells suggest that different cholinergic-like populations may be defined on the basis of the onset of neurotransmitter-related enzymes: RPO cells are first, cells in ventral periolivary regions are second, and cells associated with the LSO are last. The differences observed in the onset of ChAT and AChE expression may reflect differences in the timing of target innervation as well as differences in synaptogenesis.

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Section: General Observationsmentioning

confidence: 75%

Differential development of cholinergic-like neurons in the superior olive: a light microscopic study

Simmons¹,

Bertolotto²,

Typpo³

et al. 1999

Anatomy and Embryology

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“…Our immunohistochemical procedure was similar to that employed in our previous studies and those of others (Gilmore et al 1996;Yao and Godfrey 1998). In brief, young (250-350 g) male Sprague-Dawley rats were deeply anesthetized with sodium pentobarbital ip and perfusionfixed with 3% paraformaldehyde in PBS, pH 7.4, for light microscopic (LM) immunohistochemistry.…”

Section: Methodsmentioning

confidence: 99%

Vesicular Acetylcholine Transporter in the Rat Cochlear Nucleus: An Immunohistochemical Study

Yao

Godfrey

1999

J Histochem Cytochem.

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SUMMARY After being synthesized in the cytoplasm of axon terminals, acetylcholine is packaged into synaptic vesicles by a proton-dependent transporter, vesicular acetylcholine transporter (VAChT). Localization of VAChT is restricted to cholinergic neurons, especially their terminals. We used an anti-VAChT antibody from INCSTAR to localize cholinergic terminals in the rat cochlear nucleus (CN), an important brainstem auditory center. VAChT immunoreactivity in the rat CN appears as labeled puncta and a few connecting fibers. In ventral CN (VCN), VAChT-labeled puncta are closely associated with somatic profiles of medium to large neurons. In and near the granular regions of VCN, VAChT-labeled puncta are more diffusely scattered. In the subpeduncular corner and the medial sheet, some VAChT-labeled fibers are seen in connection with especially prominent VAChT-labeled puncta. In dorsal CN (DCN), VAChT-labeled puncta show no clear association with somata and are found in all layers. Ultrastructurally, VAChT labeling is seen in the cytoplasm and is associated with synaptic vesicle membrane of terminals with small round vesicles. Such VAChT-labeled terminals synapse with cell bodies and dendrites in the CN.

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“…The presence of neurons in the region anterior to the MSO have been indicated in rhesus and human, but no morphological details are available (Bazwinsky et al, 2003(Bazwinsky et al, ,2005Moore et al 1999). VNTB neurons utilize GABA, glycine or acetylcholine as neurotransmitter (Roberts and Ribak, 1987;Helfert et al, 1989;Yao and Godfrey, 1998). Moreover, neurons in the region of the VNTB in human are also reported to be cholinergic (Moore et al, 1999).…”

Section: Nuclei Of the Trapezoid Bodymentioning

confidence: 99%

Cytoarchitecture of the human superior olivary complex: Nuclei of the trapezoid body and posterior tier

Kulesza

2008

Hearing Research

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Immunohistochemical evaluation of cholinergic neurons in the rat superior olivary complex

Cited by 40 publications

References 41 publications

Differential development of cholinergic-like neurons in the superior olive: a light microscopic study

Differential development of cholinergic-like neurons in the superior olive: a light microscopic study

Vesicular Acetylcholine Transporter in the Rat Cochlear Nucleus: An Immunohistochemical Study

Cytoarchitecture of the human superior olivary complex: Nuclei of the trapezoid body and posterior tier

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