Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium

Summerfield, Nuala; Peters, Mary Ellen; Hercock, Carol Ann; Mobasheri, Ali; Young, Iain S.

doi:10.1016/j.jvc.2009.12.001

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…As mentioned before, SERCA1 may undergo expression in myocardium as a part of a natural adaptive process in the failing heart [15,16]. Our results are partially consistent with the results obtained by Summerfield et al [16] who noticed a higher expression of SERCA1 protein in German www.fhc.viamedica.pl trend of concomitant down-regulation of dystrophin also in the MMVD group and a negative correlation between dystrophin reactivity and features of cardiomyocyte damage were observed. It may suggest that in both DCM and MMVD, atrial tissue is exposed to various stress factors, including ischemia, which can cause loss of dystrophin in cardiomyocytes [23][24].…”

Section: Discussionsupporting

confidence: 93%

“…Although the majority of studies on SERCA2 expression in heart failure point to a decrease in the protein level, in some studies, the expression levels of SERCA2 was found to be unaltered, similarly to our results [14,16]. Regardless the unchanged level of SERCA2 expression, sarcoplasmic reticulum Ca 2+ transport function may be decreased [14].…”

Section: Discussionsupporting

confidence: 86%

“…Lack of significant difference between the DCM and control groups in our study may be due to the heterogeneity of the DCM group (apart from German Shepherds, also Dobermans, Boxer dog and Caucasian Shepherd were included). In the aforementioned study [16], authors noted also that the expression of SERCA1 protein was heterogeneous and varied regionally in the specimens obtained from right ventricle, left ventricle and interventricular septum. However, they did not examine specimens from atrial walls, what was the subject of our study.…”

Section: Sarcomeric Actinin and Smamentioning

confidence: 95%

“…SERCA1 is the isoform expressed mainly in skeletal muscles. However, there is evidence that its expression is altered in failing myocardium in humans and animals, changing the rate of Ca 2+ transport [15,16]. The changes in SERCA expression might be a part of a natural adaptive process in the failing heart [16].…”

Section: Introductionmentioning

confidence: 99%

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Cardiomyocyte marker expression in dogs with left atrial enlargement due to dilated cardiomyopathy or myxomatous mitral valve disease

Janus

Kandefer-Gola

Ciaputa

et al. 2017

Folia Histochem Cytobiol.

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Introduction. Dilated cardiomyopathy (DCM) and myxomatous mitral valve disease (MMVD) are common heart conditions in dogs. They have different etiology and pathogenesis and although other studies focused on changes in the left ventricles of the affected hearts, the aim of our study was to assess the expressions of some intrinsic proteins in the enlarged left atria. Material and methods. We performed an immunohistochemical analysis of left atrial specimens obtained from 15 dogs with DCM, 35 dogs with MMVD and six control dogs. We assessed the expression of following proteins: SERCA1, SERCA2, sarcomeric actinin, smooth muscle actin, and dystrophin. Results. We noted a higher percentage of SERCA1-positive cells in the MMVD group and lower percentage of dystrophin-positive cells in the DCM group as compared to control group. The expression of other proteins was similar in the hearts of control dogs and dogs with heart diseases. Conclusions. The observed changes in the expression patterns of some proteins in the atria of dogs with DCM and MMVD suggest that atrial enlargement relies not only on volume overload, but also alterations of the intrinsic proteins can contribute to the pathogenesis of dilated cardiomyopathy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Sarcomeric Actinin and Smamentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cardiomyocyte marker expression in dogs with left atrial enlargement due to dilated cardiomyopathy or myxomatous mitral valve disease

Janus

Kandefer-Gola

Ciaputa

et al. 2017

Folia Histochem Cytobiol.

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“…In adult heart, SERCA2 expression levels are highly associated with cardiac functions, therefore, SERCA2 could be used as a marker for detection of cardiomyopathy (Castilho et al, 2007;Andersson et al, 2009;Dai and Rabinovitch, 2009;Kontaraki et al, 2010;Stokke et al, 2010;Summerfield et al, 2010). We have shown that atp2a2a transcripts were detected strongly in the developing heart of 18-96 hpf zebrafish embryos (Fig.…”

Section: Atp2a2a Might Play Important Roles During Heart Development mentioning

confidence: 76%

Molecular structure and developmental expression of zebrafish atp2a genes

Lai

Pai²,

Tsai

et al. 2011

Genes Genom

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We isolated two atp2a genes, atp2a1 and atp2a2a, from embryonic zebrafish. Amino acid sequences deduced from zebrafish atp2a genes are aligned with orthologue proteins from other species, the results showed that they share high percentage of identities (82%-94%) and acidic pIs (5.03-5.33). Whole mount in situ hybridization experiments showed that atp2a1 and atp2a2a are maternal inherited genes which can be detected at 1-cell stage embryos and express in the entire animal pole from 6 hours post-fertilization (hpf) to 12 hpf. At the later stages (48-96 hpf), expression of atp2a1 was restricted in head and trunk muscles as well as in some neurons. In contrast to the strongly expression of atp2a1 in head muscle, expression of atp2a2a was detected in head muscle in a fainter manner. In addition, transcripts of atp2a2a were observed in the developing heart during early cardiogenesis. The present studies not only help us to comparatively analyze atp2a genes across species, but also provide useful information about expressions during early embryogenesis that will help in further investigations of functional studies of Atp2a in the future.

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Maternal diet high in linoleic acid alters offspring fatty acids and cardiovascular function in a rat model

et al. 2021

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Linoleic acid (LA), an essential n-6 fatty acid (FA), is critical for fetal growth and development. We investigated the effects of maternal high LA (HLA) diet on offspring cardiac development and how it relates to circulating FAs and cardiovascular function in adolescent offspring, and the ability of the postnatal diet to reverse any adverse effects. Female Wistar Kyoto rats were fed low LA (LLA; 1.44% energy from LA) or HLA (6.21% energy from LA) diets for 10 weeks before pregnancy, and during gestation/lactation. Offspring, weaned at postnatal day 25 (PN25), were fed LLA or HLA diets and euthanised at PN40 (n = 6-8). Maternal HLA diet decreased circulating total cholesterol and HDL-cholesterol in females and decreased total plasma n-3 FA in males, while maternal and postnatal HLA diets decreased total plasma n-3 FA in females. Alpha- linolenic acid (ALA) and eicosapentaenoic acid (EPA) were decreased by postnatal but not maternal HLA diets in both sexes. Maternal and postnatal HLA diets increased total plasma n-6 and LA, and a maternal HLA diet increased circulating leptin, in both male and female offspring. Maternal HLA decreased slopes of systolic and diastolic PVRs, and increased cardiac Col1a1, Col3a1, Atp2a1 and Notch1 in males. Maternal and postnatal HLA diets left-shifted the diastolic PVR in female offspring. Coronary reactivity was altered in females, with differential effects on flow repayment after 10–20 sec occlusions. In conclusion, maternal HLA diets impact lipids, fatty acids and cardiac function in offspring, with postnatal diet modifying fatty acids and cardiac function in the female offspring.

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Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium

Cited by 7 publications

References 25 publications

Cardiomyocyte marker expression in dogs with left atrial enlargement due to dilated cardiomyopathy or myxomatous mitral valve disease

Cardiomyocyte marker expression in dogs with left atrial enlargement due to dilated cardiomyopathy or myxomatous mitral valve disease

Molecular structure and developmental expression of zebrafish atp2a genes

Maternal diet high in linoleic acid alters offspring fatty acids and cardiovascular function in a rat model

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