Immunohistochemical expression of budding uninhibited by benzimidazole related 1 in leukoplakia and oral squamous cell carcinoma

Sravya, Taneeru; Yeluri, Sivaranjani; Guttikonda, Venkateswara Rao

doi:10.4103/0973-029x.180938

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Notably, BubR1, Mad2 and Ki-67 showed low expression in normal tissue but their expression significantly increased in the presence of dysplasia. This increasing expression was previously reported for BubR1 in oral premalignant lesions by Hsieh et al ( 18 ) and Sravya et al ( 19 ), suggesting that the deregulation of SAC is an early and important event in premalignancy. To the best of our knowledge, a significantly increased expression of Mad2 proteins, as reported in the present study, has not been described before.…”

Section: Discussionsupporting

confidence: 84%

Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study

Monteiro¹,

Silva²,

Delgado³

et al. 2021

Med Oral

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Section: Discussionsupporting

confidence: 84%

Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study

Monteiro¹,

Silva²,

Delgado³

et al. 2021

Med Oral

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Immunohistochemical Expression of BubR1 and Telomerase in Oral Squamous Cell Carcinoma

Garib¹,

Mohammad²,

Hamied³

2020

Sulaimani Dent J

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Objective: Aberrant BubR1 and Telomerase expressions are considered as important markers in tumor progression and clinical outcome. This study purposed to describe, compare, and correlate the immunohistochemical expression of BubR1 and Telomerase in oral squamous cell carcinoma (OSCC). Methods: Immunohistochemical staining was performed for 28 OSCC samples. The samples graded according to Bryne’s grading system. The reaction positivity, intracellular localization, and intensity were recorded. The expression distribution and mean-ranks of these markers were related to OSCC grades by Fisher’s exact and Kruskal Wallis tests. Finally, the correlation between them was achieved by Spearman’s rho test. Results: BubR1 was detected in 89.3% of OSCCs, equally within scores 2 and 3 (35.7%), presented with 50% faint intensity, and 64.3% cytoplasmic localization. Grade III had a significantly higher mean-rank of BubR1 scoring. Whereas Telomerase observed in 75% of OSCCs, mainly found at scores 2 and 3 (60.7%), and 42.9% was within the cytoplasm. Half of OSCCs had faint intensity. No significant differences reported in the mean-rank and stain intensity among the grades. There was no significant correlation between BubR1 and Telomerase expression. Conclusions: OSCCs had high cytoplasmic BubR1 and telomerase expression that cannot ensure proper function. Mean-rank of BubR1 expression related to OSCC grading and being more in high grade. It determines the loss of differentiation and aggressiveness of OSCC. While the mean-rank of Telomerase did not relate to histopathological grading and considered an early event in carcinogenesis, it can use for the diagnostic approach of oral carcinogenesis. These markers have an independent role in cancer progression.

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Immunohistochemical Expression of BubR1 and Telomerase in Minor Salivary Gland Tissue Adjacent to Oral Squamous Cell Carcinoma

Hamied¹

2021

SDJ

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Objectives: Evaluation of the expression of tissue markers in the parenchyma within the area adjacent to a primary tumor is critical in cancer progression, prevention, and identification of early changes in the field of cancerization. The present study aimed to evaluate BubR1 and telomerase activity in minor salivary gland tissue adjacent to oral squamous cell carcinoma (OSCC) with their clinicopathological features. Methods: Four-micron tissue sections from 21 formalin-fixed paraffin blocks of OSCC surgical margins were prepared and stained for anti-BubR1 and anti-telomerase antigens. Results: The studied sample included 71.4% males and 28.6% females, and the most prevalent site was buccal mucosa (42.9%). The total sample showed a high (71.4%) BubR1 expression, while telomerase was expressed in 42.9%. BubR1 was expressed significantly in the buccal mucosa (p=0.000) in the myoepithelial (cytoplasmic) and ductal cells (cytoplasmic and mixed). In comparison, telomerase showed significant myoepithelial mixed and cytoplasmic expression of buccal mucosa (p=0.013) and nuclear expression in ductal cells within the tongue (p=0.05). Both markers showed altered expression and predominated significantly in cases adjacent to poorly differentiated OSCC (p<0.05). Lastly, a moderate/strong correlation between the aberrant accumulation of BubR1 and telomerase was found (r=0.5-0.8, p<0.05). Conclusions: Elevated expression of BubR1 and telomerase was found in the minor salivary gland adjacent to OSCC. Therefore, they represent a valuable tool to identify molecular changes in the field of cancerization. Cases of OSCC excised from buccal mucosa and tongue required a regular follow-up, especially poorly differentiated OSCCs. The elevated expression of both markers positively regulates each other.

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Immunohistochemical expression of budding uninhibited by benzimidazole related 1 in leukoplakia and oral squamous cell carcinoma

Cited by 4 publications

References 19 publications

Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study

Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study

Immunohistochemical Expression of BubR1 and Telomerase in Oral Squamous Cell Carcinoma

Immunohistochemical Expression of BubR1 and Telomerase in Minor Salivary Gland Tissue Adjacent to Oral Squamous Cell Carcinoma

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