Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma

Abstract

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

Macroscopic and histomorphometric evaluation of different healing stages of skin wounds in horses treated with leukocyte-poor platelet-rich plasma

“…The shorter closure time of the wound UT with PRP was also reported by Monteiro et al (2009) in injuries induced on the dorsolateral surface of the metacarpal. However, a faster macroscopic healing may not result in better quality tissue (SOUZA et al, 2015). This was also observed in the present study, wherein the T wounds with LP-PRP demonstrated a superior feature of the scar tissue in the sixth biopsy, primarily with respect to the alignment of collagen fibers and fibroblasts, concluded after the assessment of the three evaluators.…”

“…Daily measurements of the retraction on the edge of wound C were also performed, which were then scanned, and the cross-sectional area (cm 2 ) was determined using QUANT v 1.0.0.28 software (VALE et al, 2003), as previously described (SOUZA et al, 2015). Finally, before and weekly after the wound induction, hematological (hemoglobin, hematocrit, erythrocyte, total platelets, and leukocytes) and biochemical (plasma total protein and fibrinogen) characteristics were evaluated in whole blood obtained by jugular venipuncture in vacuum tubes containing EDTA as the anticoagulant.…”

“…Although some studies demonstrate the effectiveness of growth factors in accelerating the healing process of skin wounds in horses (MACIEL et al, 2012), other studies have not shown this effect (MONTEIRO et al, 2009;SOUZA et al, 2014a). However, a previous report has described a better response in some constituents of the wound treated with leucocyte-poor PRP (LP-PRP) with respect to the control group (SOUZA et al, 2014b) along with better healing quality (SOUZA et al, 2015).…”

Platelet-rich plasma to treat experimentally-induced skin wounds in animals: A systematic review and meta-analysis

“…Considering the healing time, there was no statistical difference between the study groups. Two studies showed not statistical negative ES [ 47 , 53 ]; two studies showed positive ES [ 48 , 50 ] among which one with significative statistical difference [ 48 ].…”

Laserterapia Em Afecções Locomotoras: Revisão Sistemática De Estudos Experimentais

“…O mesmo foi relatado 21 dias após lesão traumática em TCC 6 . Estudos recentemente publicados em outra espécie (equina) e em outro tecido (pele) demonstraram que, dependendo da fase do processo de regeneração ou cicatrização, a expressão de colágenos determinada mediante as técnicas como histomorfometria 53 , RT-qPCR 54 e imunoistoquímica 55 , pode não se mostrar diferença entre grupos tratados e não tratados, inclusive utilizando uma terapia regenerativa como o plasma rico em plaquetas. O mesmo pode acontecer com a laserterapia e em ratos.…”