Immunohistochemical Expression of Cyclin D1 and NF - KB p65 in Oral Lichen Planus and Oral Squamous Cell Carcinoma : Comparative Study

Abid, Amer Mousa; Merza, Muna S.

doi:10.12816/0015169

Cited by 6 publications

(4 citation statements)

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“…Oncogenic effects acting on proliferating cells may induce a malignant cell pattern in OLP individuals. This result supports recent findings that imply amplification of CCND1 at both the protein and gene expression levels in OLP may impair normal cell cycle control, allowing cancer cells to develop (Yao et al, 2007;Abid and Merza, 2014).…”

supporting

confidence: 91%

“…From healthy oral tissues to dysplastic abnormalities and OSCCs, cyclin D1 levels increase sequentially (Pitiyage et al, 2009;Ramakrishna et al, 2013). Previous research has suggested that CCND1 may play a significant role in the etiology of OLP (Zhang et al, 2010;Abid and Merza, 2014). Additionally, further studies have shown changes in the expression of some cell regulatory proteins in premalignant and malignant lesions of the oral cavity (Poomsawat et al, 2010).…”

Section: Introductionmentioning

confidence: 98%

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Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Abdulhussain¹,

Alaswad²

2022

jhms

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Background: Many studies have reported that a condition initially classified as oral lichen planus (OLP) has a variable chance of developing cancer progression over time, although these results are still questionable. Cyclin D1 controls the mitotic cell cycle's transition from G1 to S. Cyclin D1 has a significant function in carcinogenesis due to its critical involvement in cell cycle control. Cyclin D1 deregulation or overexpression may result in shortened G1 phase, enhanced cell replication, and decreased reliance on growth regulators, causing disruption in conventional cell cycle progression and the progression of cancer. The aim of the current study was to evaluate the expression of Cyclin D1 in histopathologically confirmed OLP samples and to predict the rate of malignant transformation in these lesions by comparing the level of expression with some clinical parameters. Materials and Methods: The immunohistochemical procedure was used to examine the expression of cyclin D1 on paraffin-embedded sections of (40) OLP lesions and (10) specimens of normal oral mucosa. We used the SPSS software version (18.0) to do the statistical analysis in this work. Results: There is a very significant difference between the people investigated (P = 0.01), which is statistically significant. CyclinD1 was found in all of the oral lichen planus cases in the research (100%), but it was also found (80%) in the normal oral mucosa. There was also no statistically significant difference between the clinical types of OLP and the Cyclin D1 scoring (P = 0.942), which revealed that the reticular type was the most frequent (7) in mild scoring, whereas the erosive type was the most frequent in severe scoring, which accounted for the majority of the cases. Conclusion: This research found that higher cyclinD1 expression increases the risk of cancer and many of the markers should be tested. Furthermore, larger sample numbers, improved designs, and gene-reduction studies should be performed. OLP patients should be continuously monitored for OSCC progression.

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confidence: 91%

Section: Introductionmentioning

confidence: 98%

Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Abdulhussain¹,

Alaswad²

2022

jhms

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“…Cyclin D1, a protooncogene and cell-cycle regulator, is involved in the control of restriction point in the G1 phase. Abidand Merza,[ 39 ] showed cyclin D1 overexpression using immunohistochemistry in 88% oral cancer patients. The cyclin D1 expression has been correlated with histological grading in tumor staging in various types of cancers including oral carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Manoharan¹,

Rajasekaran²,

Prabhakar³

et al. 2015

Toxicol Int

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Enicostemma littorale leaves are traditionally used for the treatment of several diseases, including inflammation and cancer. This study has taken effort to explore the antitumor initiating potential of E. littorale leaves (ElELet) by analyzing the expression pattern of apoptotic (p53, Bcl-2 and Bcl-2 associated X-protein), cell-proliferative (cyclin D1 and proliferating cell nuclear antigen), angiogenic (vascular endothelial growth factor), invasive (matrix metalloproteinase-2 and 9), and inflammatory (NF-κB and cyclooxygenase-2) markers during 7, 12-dimethylbenz (a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were induced in the buccal pouches of hamsters using the potent site and organ specific carcinogen, DMBA. DMBA application 3 times a week for 14 weeks resulted in tumor formation in the buccal pouches. Hundred percent tumor formations with dysregulation in the expression pattern of apoptotic, cell proliferative, inflammatory, angiogenic, and invasive markers were observed in the buccal pouches of hamsters treated with DMBA alone. ElELet at a dose of 250 mg/kg body weight orally to DMBA treated hamsters significantly prevented the tumor formation as well as corrected the abnormalities in the expression pattern of above mentioned molecular markers. ElELet thus modulated the expression pattern of all the above mentioned molecular markers in favor of the suppression of cell proliferation occurring in DMBA induced hamster buccal pouch carcinogenesis.

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“…Cyclin D1 is a protein that is overexpressed to more than 50% in the incidence of cancer (Qie and Diehl, 2016). Overexpression of cyclin D1 causes a shortening of the G1 phase, which results in abnormal cell proliferation and additional genetic lesions (Abid and Merza, 2014). Poor prognosis of OSSC is characterised by the low cell differentiation associated with overexpression of cyclin D (Ramos-García et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of the combination of Chrysanthemum cinerariifolium (Trev.) and doxorubicin in inhibiting PI3K and Cyclin D in oral squamous cell carcinoma: in vitro study

et al. 2023

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Background: The most common type of lips and oral cavity cancer is oral squamous cell carcinoma (OSCC). Doxorubicin (DX) is commonly used as a chemotherapy drug, but its use is limited due to risk factors and drug resistance. Chrysanthemum cinerariifolium (Trev.) (CC) has potential as an anticancer agent. Combining the plant extract and chemotherapy drug might prevent OSCC proliferation by inhibiting PI3K and cyclin D protein. Therefore, the present study aimed to determine the synergistic effect of the combination of C. cinerariifolium (Trev.) and doxorubicin in inhibiting PI3K and Cyclin D protein. Methods: Human oral squamous carcinoma cell lines SCC-9 were used in this study. A cytotoxicity assay was performed to obtain the IC50 value of CC ethanol extract and DX on the SCC-9 cell line. Synergism evaluation of the combination CC and DX was analyzed using CompuSyn software. ELISA and the immunofluorescent assay were performed to determine the level of PI3K and cyclin D in the SCC-9 cell line after being treated with IC50 value of CC, IC50 value of DX and three combinations of CC and DX [7/8 IC50 CC + 1/8 IC50 DX (dose 1), 6/8 IC50 CC + 2/8 IC50 DX (dose 2), and 4/8 IC50 CC + 4/8 IC50 DX (dose 3). Results: CC stem ethanol extract and DX inhibited the proliferation of SCC-9 cell lines with the IC50 value of 133.4 µg/mL and 288.3 nM, respectively. The combination of CC and DX at dose 2 (6/8 IC50 CC + 2/8 IC50 DX) exhibited a high decrease in PI3K and cyclin D expression. Conclusions: The combination of C. cinerariifolium and doxorubicin synergistically declined OSCC proliferation by inhibiting PI3K and cyclin D expression.

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Immunohistochemical Expression of Cyclin D1 and NF - KB p65 in Oral Lichen Planus and Oral Squamous Cell Carcinoma : Comparative Study

Cited by 6 publications

References 0 publications

Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Immunohistochemical Expression of Cyclin D1 in Oral Lichen Planus

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Synergistic effect of the combination of Chrysanthemum cinerariifolium (Trev.) and doxorubicin in inhibiting PI3K and Cyclin D in oral squamous cell carcinoma: in vitro study

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