Immunohistochemical expression of endometrial L-selectin ligand is higher in donor egg recipients with embryonic implantation

Shamonki, M.; Kligman, Isaac; Shamonki, Jaime; Schattman, Glenn; Hyjek, Elizabeth; Spandorfer, Steven D.; Zaninović, Nikica; Rosenwaks, Zev

doi:10.1016/j.fertnstert.2006.04.035

Cited by 26 publications

(21 citation statements)

References 72 publications

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“…Recently, Shamonki et al [20] correlated L-selectin ligand expression with the pregnancy rate in subsequent donor egg cycles. They demonstrated significantly higher immunohistochemical reactivity for the L-selectin ligand at the apex of endometrial surface epithelium obtained during mock cycle from donor egg recipients who subsequently conceived compared to those who did not.…”

Section: Discussionmentioning

confidence: 99%

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

Foulk

Zdravkovic

Genbačev

et al. 2007

J Assist Reprod Genet

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Problem Patients with repeated implantation failure (RIF) represent a subgroup of couples who suffer from unexplained infertility. Human blastocysts utilize L-selectin to initiate implantation by binding to endometrial ligands composed of oligosaccharide moieties on the surface glycoproteins. The absence of these ligands could lead to recurrent implantation failure (RIF) in some of these couples. Methods Twenty fertile women and 20 patients with RIF were tested for the presence of the L-selectin ligands by immunohistochemistry. Endometrial biopsies were obtained on the sixth day post ovulation. After fixation, they were dated according to Noyes. Immunolocalization was performed using the MECA-79 antibody which is directed against ligands of L-selectin. Results The fertile group all showed the presence of the L-selectin ligand. Of those with RIF, five were negative for the ligand and never, despite an average of five successive embryo transfers, became pregnant. Fifteen RIF patients were positive for the L-selectin ligand, of whom ten subsequently conceived. As a screening test for RIF patients who lack the ligand, the predictive value was 100% with a sensitivity of 50% and specificity of 100%. The positive predictive value was 100% and negative predictive value is 87%. Conclusions L-selectin and its ligands play a vital role for early human implantation. Screening for the absence of the ligand may help many patients with RIF to avoid undergoing repeated failed treatment cycles.

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Section: Discussionmentioning

confidence: 99%

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

Foulk

Zdravkovic

Genbačev

et al. 2007

J Assist Reprod Genet

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“…Blocking L-selectin with specific antibodies leads to impaired adhesion of trophoblasts to the endometrial epithelium (Genbacev et al, 2003). Moreover, endometrial expression of L-selectin ligands is significantly different between fertile and infertile women in natural cycles (Foulk et al, 2007; Margarit et al, 2009), and impaired expression of L-selectin ligands reduces the chance of successful implantation (Genbacev et al, 2003; Shamonki et al, 2006). High expression of L-selectin ligands in the secretory endometrium is likely associated with robust endometrial receptivity for embryo implantation in humans (Shamonki et al, 2006; Wang et al, 2008).…”

Section: Cell-cell Interactions: the Nature Of Embryo Implantationmentioning

confidence: 99%

Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

450

480

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Embryo implantation involves the intimate interaction between an implantation-competent blastocyst and a receptive uterus, which occurs in a limited time period known as the window of implantation. Emerging evidence shows that defects originating during embryo implantation induce ripple effects with adverse consequences on later gestation events, highlighting the significance of this event for pregnancy success. Although a multitude of cellular events and molecular pathways involved in embryo-uterine crosstalk during implantation have been identified through gene expression studies and genetically engineered mouse models, a comprehensive understanding of the nature of embryo implantation is still missing. This review focuses on recent progress with particular attention to physiological and molecular determinants of blastocyst activation, uterine receptivity, blastocyst attachment and uterine decidualization. A better understanding of underlying mechanisms governing embryo implantation should generate new strategies to rectify implantation failure and improve pregnancy rates in women.

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“…The pattern and intensity of protein and mRNA staining for VEGF, VEGFR-2, ANG-1, ANG-2, and TIE-2 in follicular tissue were determined semi-quantitatively using a histochemical score (HSCORE) method that has been widely reported in a number of studies in our own laboratory [27][28][29][30] and by others [31,32]. Moreover, the HSCORE method of analysis used in this study has been previously validated as a suitable method for semiquantitative estimation of immunohistochemistry and in situ hybridization results [33].…”

Section: Quantification Of Staining For Immunohistochemistry and In Smentioning

confidence: 99%

Progesterone exposure of seasonally anoestrous ewes alters the expression of angiogenic growth factors in preovulatory follicles

2014

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Immunohistochemical expression of endometrial L-selectin ligand is higher in donor egg recipients with embryonic implantation

Cited by 26 publications

References 72 publications

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

Physiological and molecular determinants of embryo implantation

Progesterone exposure of seasonally anoestrous ewes alters the expression of angiogenic growth factors in preovulatory follicles

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