Immunohistochemical Expression of Epithelial-Mesenchymal Transition Markers in Early Gastric Cancer: Cancer Tissue versus Noncancer Tissue

Jung, Hyun-Joo; Hong, Su Jin; Kim, Shin Hee

doi:10.5946/ce.2018.181

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…As regard E-Cadherin expression pattern, there was a statistically signi cant difference between chronic gastritis and GC cases with the lowest percentage of homogenous expression in GC cases. These ndings were consistent with others who suggest the role of E-Cadherin in multistep carcinogenesis [41,42].…”

Section: Discussionsupporting

confidence: 93%

Gastric Carcinoma with low ROR alpha, low E- Cadherin and High LAPTM4B Immunohistochemical Profile; is associated with poor prognosis in Egyptian patients

Mashad

Kandil

Talab

et al. 2022

Preprint

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Background Gastric carcinoma (GC) is the tenth most prevalent cancer in both sexes in Egypt. Many pathways have been investigated regarding pathogenesis of GC, including epithelial-mesenchymal transition (EMT) pathway. In view of multiplicity of carcinogenic pathways, poor survival and chemotherapy resistance detected in GC patients, more analysis of these pathways is required for better molecular selection of patients, prediction of prognosis and developing new therapeutic targets. Down-regulation of E-Cadherin is an important EMT stage. RORα is a tumor suppressor gene, expressed in normal epithelial tissues and reduced in a variety of human cancers. Knocking down of RORα; increase cell proliferation, EMT, migration, and invasion. LAPTM4B is a protooncogene and it has been suggested to be strictly associated with EMT induction. Therefore, this work aims to investigate the role of RORα, LAPTM4B and E-Cadherin and its relationship to prognosis of GC. Methods This is a retrospective study where the standard immunohistochemical technique was done to assess the expression of RORα, LAPTM4B and E-Cadherin in 167 cases of chronic gastritis (control group) and 73 primary gastric carcinomas (51 of them have available adjacent non tumor tissue). Results Low RORα and high LAPTM4B expression in GC cases were associated with unfavorable prognostic factors such as positive lymph nodes, and high tumor budding. E-Cadherin Heterogeneous staining was associated with poor prognostic pathological criteria, such as diffuse type GC and high tumor budding. In GC, there was significant co parallel correlation between RORα and E-Cadherin expression while LAPTM4B showed inverse correlation with E-Cadherin expression. Low RORα, high LAPTM4B, and negative or heterogeneous E-Cadherin were the most common immunohistochemical profile in GC cases. Low RORα expression showed poor prognostic impact on overall patient survival. Conclusions Low RORα H-score and increased expression of LAPTM4B were significantly associated with unfavorable prognostic parameters of GC which may indicate their crucial role in tumor aggressiveness. The predominance of low RORα, high LAPTM4B and heterogeneous or negative E-Cadherin immunohistochemical profile in GC cases with unfavorable pathological parameters suggested that this profile may predict tumor behavior and this profile could be linked to EMT molecular subtype of GC

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Section: Discussionsupporting

confidence: 93%

Gastric Carcinoma with low ROR alpha, low E- Cadherin and High LAPTM4B Immunohistochemical Profile; is associated with poor prognosis in Egyptian patients

Mashad

Kandil

Talab

et al. 2022

Preprint

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“…In this respect, our preliminary results revealed a significantly higher value of α-SMA in CSCs subpopulation in comparison to parental cells. These results could be supported by previous studies on basal cell carcinoma, gastric, ovarian, and prostate cancer in which, the higher expression of α-SMA in malignant cells was significantly associated with increased tumorigenesis, migration, and invasion ability, indicating its possible role in CSC biology (Anggorowati et al, 2017;Jung et al, 2019;Jabour et al, 2022;Masola et al, 2022). A recent study by Jabour et al (2022) reported that the aggressive biological behavior of basal cell carcinoma can be predicted using α-SMA biomarker.…”

Section: Cd44 Positive Cancer Stem Cells Express Invasion and Metasta...supporting

confidence: 76%

CD44 positive cancer stem cells express invasion and metastasis markers: Analysis of three tongue squamous cell carcinoma cell lines

Kamal,

Riad,

Abdel Latif

et al. 2024

Egyptian Dental Journal

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Objectives: This study aimed to analyze the expression of epithelial-mesenchymal transition (EMT) markers, E-cadherin, and alpha-smooth muscle actin (α-SMA) in CD44+ cancer stem cells (CSCs) compared to parental cells to shed light on the possible role of CSCs in the invasion and metastasis of tongue squamous cell carcinoma (TSCC).Materials and Methods: Three tongue squamous cell carcinoma cell lines were used in this study. Cells were cultured for cancer stem cell screening and isolation. Tumor cells underwent CD44-based fluorescence-activated cell sorting analysis. Subsequently the expression of E-cadherin and α-SMA in CSCs sub-population and parental cells was evaluated using quantitative real-time PCR. Results:The three cell lines displayed stable CD44 expression levels. Moreover, E-cadherin recorded a significantly higher value in parental cells compared to CSCs sub-population, whereas α-SMA recorded a significantly lower value in parental cells compared to CSCs sub-population. A very strong negative correlation was noted between E-cadherin and each of α-SMA and CD44, whereas a very strong positive correlation was noted between α-SMA and CD44. Conclusion:The findings of our study indicated that TSSC harbors a sub-population of CD44+ CSCs that exhibited enhanced EMT features compared to other cell populations. Our data provide new evidence in support of a potential link between α-SMA expression and CSC features which may have relevant implications for the development of cancer stem cell-based treatment options.

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“…Gastric cancer (GC) is a malignancy that occurs in gastric epithelial tissue [ 1 ], and its incidence accounts for 40%–50% of gastrointestinal cancers, ranking first in gastrointestinal tumors [ 2 ]. Additionally, its morbidity and mortality rank second among malignant tumors worldwide, and its morbidity and mortality rank first among all cancers in China [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-522-3p Targets Transcription Factor 4 to Overcome Cisplatin Resistance of Gastric Cells

Guo¹,

Xue²,

Ni³

et al. 2022

Journal of Oncology

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Gastric cancer (GC) is a malignancy originating from gastric epithelial tissue. Chemoresistance to cisplatin (DDP) often leads to chemotherapy failure in GC. Previously, miR-522 was found to be associated with chemoresistance in GC cells. Thus, we attempted to clarify miR-522-3p’s role underlying chemoresistance of GC cells. RT-qPCR measured the miR-522-3p levels in untreated and DDP-treated AGS cells. RT-qPCR and Western blotting detected transcription factor 4 (TCF4) mRNA and protein levels in GC cells. AGS and AGS/DDP cell proliferation were detected by the colony formation assay. Flow cytometry analysis detected AGS and AGS/DDP cell apoptosis. Bioinformatics and dual luciferase reporter assays predicted and verified the relationship between miR-522-3p and TCF4. Rescue experiments further clarified the regulatory patterns of miR-522-3p/TGF4 in GC cells. miR-522-3p presented a downregulation in GC cells and was positively affected by DDP. TCF4 presented elevation in GC cells and was negatively affected by DDP. Mechanistically, miR-522-3p targeted TCF4 to suppress TCF4 gene expression. miR-522-3p overexpression suppressed GC cell proliferation and resistance to DDP and GC cell apoptosis was facilitated. TCF4 overexpression facilitated GC cell proliferation and resistance to DDP while repressing GC cell apoptosis. TCF4 elevation rescued changes in GC cell proliferation, apoptosis, and chemoresistance due to miR-522-3p overexpression. To sum up, miR-522-3p suppresses GC cell malignancy and resistance to DDP via targeting TCF4. Our research may provide a new biomarker for GC diagnosis and a novel direction for GC chemotherapy.

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Immunohistochemical Expression of Epithelial-Mesenchymal Transition Markers in Early Gastric Cancer: Cancer Tissue versus Noncancer Tissue

Cited by 7 publications

References 21 publications

Gastric Carcinoma with low ROR alpha, low E- Cadherin and High LAPTM4B Immunohistochemical Profile; is associated with poor prognosis in Egyptian patients

Gastric Carcinoma with low ROR alpha, low E- Cadherin and High LAPTM4B Immunohistochemical Profile; is associated with poor prognosis in Egyptian patients

CD44 positive cancer stem cells express invasion and metastasis markers: Analysis of three tongue squamous cell carcinoma cell lines

MiR-522-3p Targets Transcription Factor 4 to Overcome Cisplatin Resistance of Gastric Cells

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