Immunohistochemical Expression of Estrogen and Progesterone Receptors in Human Colorectal Adenoma and Carcinoma Using Specified Automated Cellular Image Analysis System: A Clinicopathological Study

Qasim, Ban J.; Ali, Hussam Hasson

doi:10.5001/omj.2011.78

Cited by 12 publications

(14 citation statements)

References 17 publications

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“…In addition, there are also studies showing that estrogen-only hormone replacement therapy does not produce changes in the prevalence or survival after developing colon cancer in women. The authors postulate that P4 is necessary for protection against this type of cancer because of the modulation that exerts on the effects of estrogens on carcinogenesis (16).…”

Section: Discussionmentioning

confidence: 99%

“…However, other studies report the implication of P4. For example, the expression of PR increases in the order of normal colon-adenoma-adenocarcinoma, supporting its role on this disease (16). Furthermore, some studies propose synthetic progestins as chemopreventive agents in colon cancer (17), but little is known about the role of natural P4.…”

Section: Introductionmentioning

confidence: 97%

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Estradiol and progesterone regulate proliferation and apoptosis in colon cancer

Sasso

Santiano

Arboccó

et al. 2019

Endocrine Connections

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Epidemiological studies describe estrogens as protectors in the development of colon cancer in postmenopausal women treated with hormone replacement therapy. However, the role of progesterone in colon cancer has been minimally studied and the results are controversial. For the above, the objective of this work was to determine the hormonal regulation exerted by natural ovarian steroids on proliferation and apoptosis in an experimental model of colon cancer in ovariectomized rats treated with 17-beta estradiol and progesterone. Sprague–Dawley rats were exposed to the carcinogen 1,2-dimethylhydrazine to induce colon tumors. Thirty days later, the rats were ovariectomized and treated with estradiol (60 μg/kg), progesterone (10 mg/kg), estradiol plus progesterone (60 μg/kg and 10 mg/kg) or vehicle. We observed no significant differences in colon cancer incidence and tumor multiplicity between the groups. Nevertheless, we observed a decrease in PCNA expression and a greater number of apoptotic index, higher expression of caspase 3, cleaved PARP and cleaved caspase 8 in tumors, confirming the activation of the extrinsic pathway of apoptosis by the combined treatment. In addition, we observed a higher expression of estrogen receptor beta in these tumors. We conclude that the action of both hormones, estradiol and progesterone, is necessary to reduce proliferation and increase apoptosis in colon tumors, probably through estrogen receptor beta activation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Estradiol and progesterone regulate proliferation and apoptosis in colon cancer

Sasso

Santiano

Arboccó

et al. 2019

Endocrine Connections

View full text Add to dashboard Cite

show abstract

“…[22][23][24] The preparation of cytological specimens to be examined immunocytochemically was the same procedure as that of conventional cytology. [22][23][24] The preparation of cytological specimens to be examined immunocytochemically was the same procedure as that of conventional cytology.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Sensitivity and Specificity of Immunohistochemical Markers in the Differential Diagnosis of Effusion Cytology

Yahya¹,

Ali²,

Hussein³

2013

Oman Med J

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“…Instead of adding a totally new assay to laboratory operation, it modifies only the quantifying step while keeping the previous immunohistochemical assay steps unchanged. [6]…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of matrix metalloproteinase-7 in human colorectal adenomas using specified automated cellular image analysis system: A clinicopathological study

Qasim

Ali

2013

Saudi J Gastroenterol

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Background/Aim:To evaluate the immunohistochemical expression of matrix metalloproteinase-7 (MMP-7) in colorectal adenomas, and to correlate this expression with different clinicopathological parameters.Patients and Methods:The study was retrospectively designed. Thirty three paraffin blocks from patients with colorectal adenoma and 20 samples of non-tumerous colonic tissue taken as control group were included in the study. MMP-7 expression was assessed by immunohistochemistry method. The scoring of immunohistochemical staining was conducted utilizing a specified automated cellular image analysis system (Digimizer).Results:The frequency of positive immunohistochemical expression of MMP-7 was significantly higher in adenoma than control group (45.45% versus 10%) (P value < 0.001). Strong MMP-7 staining was mainly seen in adenoma cases (30.30%) in comparison with control (0%) the difference is significant (P < 0.001). The three digital parameters of MMP-7 immunohistochemical expression (Area (A), Number of objects (N), and intensity (I)) were significantly higher in adenoma than control. Mean (A and I) of MMP-7 showed a significant correlation with large sized adenoma (≥ 1cm) (P < 0.05), also a significant positive correlation of the three digital parameters (A, N, and I) of MMP-7 expression with villous configuration and severe dysplasia in colorectal adenoma had been identified (P < 0.05).Conclusion:MMP-7 plays an important role in the growth and malignant conversion of colorectal adenomas as it is more likely to be expressed in advanced colorectal adenomatous polyps with large size, severe dysplasia and villous histology. The use of automated cellular image analysis system (Digmizer) to quantify immunohistochemical staining yields more consistent assay results, converts semi-quantitative assay to a truly quantitative assay, and improves assay objectivity and reproducibility.

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Immunohistochemical Expression of Estrogen and Progesterone Receptors in Human Colorectal Adenoma and Carcinoma Using Specified Automated Cellular Image Analysis System: A Clinicopathological Study

Abstract: ER and PR expression increased in a sequence of; normal colonic mucosa-adenoma-carcinoma, and a positive correlation was observed between ER and PR expression in colonic adenoma and carcinoma specimen indicating that ER and PR may play a role in colorectal carcinogenesis.

Cited by 12 publications

References 17 publications

Estradiol and progesterone regulate proliferation and apoptosis in colon cancer

Estradiol and progesterone regulate proliferation and apoptosis in colon cancer

Evaluation of the Sensitivity and Specificity of Immunohistochemical Markers in the Differential Diagnosis of Effusion Cytology

Immunohistochemical expression of matrix metalloproteinase-7 in human colorectal adenomas using specified automated cellular image analysis system: A clinicopathological study

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