Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas

Mandrioli, Luciana; Panarese, Serena; Cesari, Alessandro; Mandara, Maria Teresa; Marcato, Paolo Stefano; Bettini, Giuliano

doi:10.4142/jvs.2007.8.2.111

Cited by 20 publications

(27 citation statements)

References 10 publications

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“…Maintenance of telomere length by telomerase is a carcinogenetic mechanism for many neoplasms. 8,16,23,28,37 Mast cell precursors undergo transient growth factor-mediated induction of telomerase activity during differentiation. 6 Human MCT cell lines tend to maintain a persistently high telomerase activity, uninfluenced by protein kinase or cell cycle inhibitors.…”

Section: Discussionmentioning

confidence: 99%

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Prognostic Value of Histologic and Immunohistochemical Features in Feline Cutaneous Mast Cell Tumors

Sabattini

Bettini

2010

Vet Pathol

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Feline cutaneous mast cell tumors (MCTs) have been histologically classified as mastocytic (well differentiated or pleomorphic) and atypical/poorly granulated. Their biologic behavior ranges from benign to malignant, but prognostic factors are not well defined. Histologic classification, number of tumors, mitotic index, cytoplasmic granularity, and infiltration by eosinophils or lymphocytes were evaluated retrospectively in 25 feline cutaneous MCTs. Immunohistochemistry was applied to assess KIT (CD117) pattern and immunoreactivity score, telomerase expression (human telomerase reverse transcriptase), and proliferation index (MIB-1/Ki67 index). Case outcome was obtained via telephone interviews. The tumors comprised 15 mastocytic well-differentiated, 7 mastocytic pleomorphic, and 3 atypical/poorly granulated MCTs. Immunohistochemically, CD117 was expressed in 13 of 25 tumors (52%), and telomerase reverse transcriptase was expressed in 15 of 22 (68%), with no correlation to histologic classification. Mitotic index, KIT immunoreactivity score, and Ki67 index were significantly higher in mastocytic pleomorphic MCTs than in the other 2 categories. Five cats (20%) died of tumor-related causes. Multiplicity of lesions, pleomorphic phenotype, KIT immunoreactivity score, and mitotic and Ki67-indices correlated with an unfavorable outcome. Mitotic index was the strongest predictive variable. These results suggest that histologic classification, CD117/KIT immunohistochemistry, and proliferation indices may help to identify potentially aggressive cases of feline cutaneous MCT. Aberrant KIT protein localization and telomerase immunoreactivity warrant further exploration as potential prognostic markers.

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Section: Discussionmentioning

confidence: 99%

“…23,28 Human mast cell precursors have transient induction of telomerase activity, 6 but telomerase expression in feline MCTs has not been assessed.…”

mentioning

confidence: 99%

Prognostic Value of Histologic and Immunohistochemical Features in Feline Cutaneous Mast Cell Tumors

Sabattini

Bettini

2010

Vet Pathol

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“…The cellular immortality‐related gene telomerase reverse transcriptase has been shown to be expressed in a variety of canine and feline brain tumors, and similar to human tumors has been associated with tumor grade 119, 120, 121. Similarly, overexpression of MMP‐2 and ‐9, E‐cadherin, claudin‐1, IL‐6, and cyclooxygenase‐2 has been documented in canine and feline meningiomas,121, 122, 123, 124, 125, 126 and MMP‐2/9 has been identified in canine gliomas and choroid plexus tumors 125.…”

Section: Molecular Diagnosticsmentioning

confidence: 99%

Advances in Diagnostic and Treatment Modalities for Intracranial Tumors

Dickinson

2014

Veterinary Internal Medicne

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Intracranial neoplasia is a common clinical condition in domestic companion animals, particularly in dogs. Application of advances in standard diagnostic and therapeutic modalities together with a broad interest in the development of novel translational therapeutic strategies in dogs has resulted in clinically relevant improvements in outcome for many canine patients. This review highlights the status of current diagnostic and therapeutic approaches to intracranial neoplasia and areas of novel treatment currently in development.

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“…25 Canine normal testis was used as a positive control for h-TERT. 26 An isotype-matched IgG negative control was run on each section.…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that a high proliferative index in the tumor is associated with low progesterone receptor (PR) concentration, suggesting that PR expression in canine and feline meningiomas is a reliable prognostic factor in the evaluation of this tumor, as well as in humans. 2,12,25,46 More recently, canine and feline meningiomas have been tested for telomerase immunoreactivity 22,26 and have produced contradictory results as to the correlation with the proliferative index.…”

Section: Introductionmentioning

confidence: 99%

Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Expression in Canine and Feline Meningioma

et al. 2009

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Abstract. Fifty-one meningiomas obtained from 28 dogs and 23 cats were selected for this study to investigate the immunohistochemical expression of matrix metalloproteinase (MMP)-2 and MMP-9 and to compare it to the reverse transcriptase subunit of human-telomerase, progesterone receptor expression, and the proliferative index of the tumors, expressed by Ki67 and proliferating cellular nuclear antigen. Paraffin-embedded tumor tissue was obtained from biopsy samples (28 cases) and at necropsy (23 cases). The most common histotype was malignant in dogs (12/28) and transitional in cats (12/23). Slides immunolabelled for MMPs showed a diffuse cytoplasmic pattern. Twenty-one cases (19 dogs and 2 cats) did not express MMP-2, while only 2 cases were completely negative for MMP-9. The highest values of MMP-2 and MMP-9 were observed in a psammomatous and meningothelial tumor, respectively. On statistical analysis, MMP-2 expression did not show a significant correlation with MMP-9. Moreover, both MMP expressions failed to show significant variance among histologic patterns of the tumor and correlation with the proliferative index. MMP immunolabeling showed an inconstant correlation with progesterone receptor expression. No significant correlation was found between MMP and reverse transcriptase subunit of human-telomerase expression. In feline meningiomas, the MMP-2 value was significantly higher than in canine tumors and the MMP-9 value tended to be low for meningiomas with a follow-up duration from the 23 rd month to the 44 th month. In cats, the longer the time from surgery, the lower the proliferative index seemed to be. In dogs, we failed to find a correlation between MMP expression and the follow-up duration.

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Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas

Cited by 20 publications

References 10 publications

Prognostic Value of Histologic and Immunohistochemical Features in Feline Cutaneous Mast Cell Tumors

Prognostic Value of Histologic and Immunohistochemical Features in Feline Cutaneous Mast Cell Tumors

Advances in Diagnostic and Treatment Modalities for Intracranial Tumors

Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Expression in Canine and Feline Meningioma

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