Immunohistochemical expression of heat shock protein 70 in vitiligo

Abdou, Asmaa Gaber; Maraee, Alaa Hassan; Reyad, Walla

doi:10.1016/j.anndiagpath.2012.11.005

Cited by 24 publications

(22 citation statements)

References 28 publications

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“…Moreover, we found that the serum level of hsCRP did not differ between the three groups with respect to sex and BMI. In a previous study, the researchers found that type A and B vitiligo have different pathogenesis and the autoimmune theory is only applicable to type a [13] which is consistent with our findings.…”

Section: Discussionsupporting

confidence: 83%

“…Since these markers are inflammatory markers, it can be stated that inflammation has a key role in the pathogenesis of vitiligo, especially generalized vitiligo, and hsCRP is an inflammatory marker that increases in systemic inflammation [6]. Moreover, the serum level of hsp-70 (an acute phase protein) increases significantly in patients with vitiligo [13].…”

Section: Discussionmentioning

confidence: 99%

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A New Biomarker in Patients with Vitiligo: A Case-Control Study

Ghaderi¹

2016

MOJI

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Background: CRP is an acute phase protein secreted in the blood stream by the liver in response to inflammatory cytokines such as IL6 and several other systemic inflammation biomarkers. Since inflammatory and immune factors have a key role in the pathogenesis of vitiligo, we aimed to assess the relationship between the serum level of hsCRP (as a biomarker for systemic inflammation) and the pathogenesis of vitiligo. Methods:In this case-control study, we enrolled patients who had referred to the Dermatology Department of Valiasr Dermatology Hospital, Birjand, Iran, during 2013. The patients were diagnosed to have vitiligo by a dermatologist. The patients were divided into two groups: those with type A vitiligo (generalized, n=30) and those with type B vitiligo (segmental, n=30). Moreover, 30 people who had the inclusion criteria and did not have vitiligo were selected among those referring to the clinic as the control group and matched with the other two groups. The serum hsCRP levels were checked for all the patients in the three groups and compared. Data were compared between the three groups and analyzed using SPSS software. Results:We did not find any significant difference between the three groups with respect to sex, age, smoking habit, BMI, and history of diabetes mellitus. The serum level of hsCRP was 4.76±1.31 mg/l in patients with type a vitiligo, 3.71±1.03 in those with type B vitiligo, and 3.01±1.08 in those in the control group. The highest level of serum hsCRP was related to patients with type A vitiligo while the lowest mean was related to the control group. The mean serum hsCRP level was significantly higher in patients with type A compared with those with type B and the control group (P<0.001). However, in patients with type B and the control groups no significant different was seen in this regard (P=0.053). Moreover, we found that the serum level of hsCRP did not differ between the three groups with respect to BMI. Conclusion:We found an association between increased serum hsCRP level and generalized vitiligo. The usage of hsCRP alone is neither sensitive, nor specific in diagnosis of vitiligo but this association could imply that hsCRP could intensify the severity of vitiligo thus serum hsCRP level might be useful for evaluating the disease activity of vitiligo as Novel biomarker.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

A New Biomarker in Patients with Vitiligo: A Case-Control Study

Ghaderi¹

2016

MOJI

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“…This supports the convergence and melanocytorrhagy theories which state that autoimmunity, impaired redox status, and genetic predisposition all contribute to vitiligo development (Kumar and Parsad, 2012;Le Poole et al, 1993b). It was reported that HSP70 is overexpressed in vitiligo perilesional and lesional skin compared with unaffected areas (Abdou et al, 2013). For that study, an antibody was used that cannot distinguish between constitutive and inducible HSP70.…”

Section: Discussionsupporting

confidence: 63%

“…It was reported that HSP70 is overexpressed in vitiligo perilesional and lesional skin compared with unaffected areas (Abdou et al., ). For that study, an antibody was used that cannot distinguish between constitutive and inducible HSP70.…”

Section: Discussionmentioning

confidence: 99%

Preferential secretion of inducible HSP70 by vitiligo melanocytes under stress

Mosenson

Flood

Klarquist

et al. 2014

Pigment Cell Melanoma Res

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SUMMARY Inducible HSP70 (HSP70i) chaperones peptides from stressed cells, protecting them from apoptosis. Upon extracellular release, HSP70i serves an adjuvant function, enhancing immune responses to bound peptides. We questioned whether HSP70i differentially protects control and vitiligo melanocytes from stress and subsequent immune responses. We compared expression of HSP70i in skin samples, evaluated the viability of primary vitiligo and control melanocytes exposed to bleaching phenols, and measured secreted HSP70i. We determined whether HSP70i traffics to melanosomes to contact immunogenic proteins by cell fractionation, western blotting, electron microscopy and confocal microscopy. Viability of vitiligo and control melanocytes was equally affected under stress. However, vitiligo melanocytes secreted increased amounts of HSP70i in response to MBEH, corroborating with aberrant HSP70i expression in patient skin. Intracellular HSP70i colocalized with melanosomes, and more so in response to MBEH in vitiligo melanocytes. Thus whereas either agent is cytotoxic to melanocytes, MBEH preferentially induces immune responses to melanocytes.

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“…Thus, its expression was expected, although no differences were observed between lesions from Balb/c and C57BL/6 mice. Hsp70 is one of the heat shock glycoproteins that stabilize intracellular processes of cells under temperature changes or stress, its expression has been reported in various models of injury, including skin excision, laser and ultraviolet irradiation and vitiligo [1,57]. Thus, it is not surprising that cells within leishmanial lesions expressed HSP70.…”

Section: Discussionmentioning

confidence: 97%

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

Araújo

Giorgio

2015

Arch Dermatol Res

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Leishmanioses are chronic parasitic diseases and host responses are associated with pro- or anti-inflammatory cytokines involved, respectively, in the control or exacerbation of infection. The relevance of other inflammatory mediators and stress markers has not been widely studied and there is a need to search for biomarkers to leishmaniasis. In this work, the stress and inflammatory molecules p38 mitogen-activated protein kinase, cyclooxygenase-2, migration inhibitory factor, macrophage inflammatory protein 2, heat shock protein 70 kDa, vascular endothelial factor (VEGF), hypoxia-inducible factors (HIF-1α and HIF-2α), heme oxygenase and galectin-3 expression were assessed immunohistochemically in self-controlled lesions in C57BL/6 mice and severe lesions in Balb/c mice infected with Leishmania amazonensis. The results indicated that the majority of molecules were expressed in the cutaneous lesions of both C57BL/6 and Balb/c mice during various phases of infection, suggesting no obvious correlation between the stress and inflammatory molecule expression and the control/exacerbation of leishmanial lesions. However, the cytokine VEGF was only detected in C57BL/6 footpad lesions and small lesions in Balb/c mice treated with antimonial pentavalent. These findings suggest that VEGF expression could be a predictive factor for murine leishmanial control, a hypothesis that should be tested in human leishmaniosis.

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Immunohistochemical expression of heat shock protein 70 in vitiligo

Cited by 24 publications

References 28 publications

A New Biomarker in Patients with Vitiligo: A Case-Control Study

A New Biomarker in Patients with Vitiligo: A Case-Control Study

Preferential secretion of inducible HSP70 by vitiligo melanocytes under stress

Immunohistochemical evidence of stress and inflammatory markers in mouse models of cutaneous leishmaniosis

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