Immunohistochemical expression of interferon-γ in different types of granulomatous lesions associated with bovine paratuberculosis

Fernández, Miguel; Fuertes, M.; Elguezabal, Natalia; Castaño, Pablo; Royo, M.; Ferreras, M.C.; Benavides, Julio; Pérez, Valentín Pérez

doi:10.1016/j.cimid.2017.01.002

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…In this sense, only the Peyer patches and mesenteric lymph node from NVI group produced a slightly higher IFNγ production. This result is consistent with the greater number of granulomas detected in this group as an increased number of granulomatous lesions have been related to a higher number of IFN-γ-immunolabeled cells (6) and are likely that lesions present in the remaining infected groups might not be sufficient to generate a substantial local cell-mediated immune response in response to mycobacterial antigens. Therefore, neither peripheral cellular and humoral immune responses nor evaluation of the relative proportion of lymphocyte subpopulations in whole blood and tissues nor local production of IFN-γ was able to predict those animals that remain unprotected early after infection.…”

Section: Discussionsupporting

confidence: 86%

Influence of Heterologous and Homologous Vaccines, and Their Components, on the Host Immune Response and Protection Against Experimental Caprine Paratuberculosis

et al. 2022

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Vaccination against paratuberculosis, a chronic disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map), has been considered as the most effective control method. However, protection is incomplete, and the mechanisms operating in the response of the animals to vaccination are not fully understood. Therefore, this study analyzed the immune response and the effects on protection against Map infection, elicited by paratuberculosis (Silirum®) and tuberculosis (heat-inactivated M. bovis [HIMB]) vaccines and their components in a caprine experimental model. Fifty goat kids were divided into 10 groups (n = 5) according to their vaccination (Silirum®, HIMB and nonvaccinated), immunization (inactivated bacteria or adjuvant), and/or infection. Oral challenge with Map was performed 45 days postvaccination/immunization (dpv), and animals were euthanized at 190 dpv. Peripheral immune response and proportion of lymphocyte subpopulations were assessed monthly by enzyme-linked immunosorbent assay and flow cytometry analysis, respectively. Local immune response, proportion of tissue lymphocyte subpopulations, Map detection (polymerase chain reaction), and histological examination were conducted in gut-associated lymphoid tissues. All infected groups developed paratuberculosis granulomatous lesions despite vaccination or immunization. The Silirum® and HIMB-vaccinated groups showed a considerable lesion reduction consistent with a significant peripheral cellular and humoral immune response. Besides, a lower number of granulomas were observed in groups immunized with inactivated bacteria and adjuvants in comparison to nonvaccinated and infected group. However, despite not being significant, this reduction was even higher in adjuvant immunized groups, which developed milder granulomatous lesion with no detectable peripheral immune responses associated with immunization. No changes in the peripheral and local proportion of lymphocyte subsets or local immune response were detected in relation to either vaccination/immunization or infection. Despite that paratuberculosis and tuberculosis vaccination showed a partial and cross-protection against Map infection, respectively, only histological examination could assess the progression of infection in these animals. In addition, the pattern observed in the reduction of the lesions in adjuvant immunized groups suggests the possible involvement of a nonspecific immune response that reduces the development of granulomatous lesions.

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Section: Discussionsupporting

confidence: 86%

Influence of Heterologous and Homologous Vaccines, and Their Components, on the Host Immune Response and Protection Against Experimental Caprine Paratuberculosis

et al. 2022

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“…For formalin-fixed tissue specimens in specific cases, antigen retrieval may be necessary. Antigen retrieval methods using enzymatic or heat-based protocols vary according to each molecular target and comprise: heat-induced (heating by pressure cookers or microwave in citrate buffer for 10 to 20 min), enzymatic (0.1% trypsin or pepsin in phosphate-buffered saline for 30 min at 37°C) or proteinase K (10 μg/mL proteinase K in Tris buffer for 15 min) may be necessary [163][164][165][166][167][168].…”

Section: Immunohistochemistry and Immunofluorescent Characterization Of Macrophagesmentioning

confidence: 99%

“…Immunohistochemistry and immunofluorescence have been utilized to identify, enumerate and characterize tissue macrophages in formalin-fixed [160,[163][164][165][166][167][175][176][177] or frozen [168][169][170][172][173][174] sections of a variety of bovine tissue samples including adipose tissue [160,167,168,175], in the intestinal tissue of cattle with spontaneous paratuberculosis at different stages of the disease [165,166,[172][173][174], the endometrium of pregnant and post parturient dairy cows [169,170,176], fibrotic liver lesions in bovines infected with Fasciola hepatica [163], lung lesion in bovine contagious pleuropneumonia [177] and early lesions during experimental M. bovis aerosol infection [164].…”

Section: Primarymentioning

confidence: 99%

“…Studies on paratuberculosis have demonstrated significantly higher numbers of M2 macrophages in the ileum of bovines with the clinical stage multibacillary lesions [165,173]. On the contrary, significant polarization versus M1 phenotype has been observed in paucibacillary lesions [166]. In lung lesions of contagious bovine pleuropneumonia, a general M1 polarization has been detected [177].…”

Section: Primarymentioning

confidence: 99%

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Methods in isolation and characterization of bovine monocytes and macrophages

Ceciliani

Morales

Matteis

et al. 2021

Methods

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Monocytes and macrophages belong to the mononuclear phagocyte system and play important roles in both physiological and pathological processes. The cells belonging to the monocyte/macrophage system are structurally and functionally heterogeneous. Several subsets of monocytes have been previously identified in mammalian blood, generating different subpopulations of macrophages in tissues. Although their distribution and phenotype are similar to their human counterpart, bovine monocytes and macrophages feature differences in both functions and purification procedures. The specific roles that monocytes and macrophages fulfil in several important diseases of bovine species, including among the others tuberculosis and paratuberculosis, brucellosis or the disease related to peripartum, remain still partially elusive. The purpose of this review is to discuss the current knowledge of bovine monocytes and macrophages. We will describe methods for their purification and characterization of their major functions, including chemotaxis, phagocytosis and killing, oxidative burst, apoptosis and necrosis. An overview of the flow cytometry and morphological procedures, including cytology, histology and immunohistochemistry, that are currently utilized to describe monocyte and macrophage main populations and functions is presented as well.

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“…IFN-γ is one of the key cytokines in mycobacterial diseases and it induces M1 polarization of macrophages, leading to a pro-inflammatory response and increased microbicidal capacity. Macrophage bactericidal activity and phagocytosis are significantly enhanced by IFN-γ when it acts in concert with TNF-α, being necessary for M. tuberculosis control [46,47].…”

Section: Discussionmentioning

confidence: 99%

Alternative Vaccination Routes against Paratuberculosis Modulate Local Immune Response and Interference with Tuberculosis Diagnosis in Laboratory Animal Models

Arrazuria

Ladero

Molina

et al. 2020

Veterinary Sciences

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Paratuberculosis (PTB) is an enteric granulomatous disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) that mainly affects ruminants. Current vaccines have shown to be cost–effective control reagents, although they are restricted due to cross-interference with bovine tuberculosis (bTB). Therefore, novel vaccination strategies are needed and this study is focused on evaluating alternative vaccination routes and their effect on the local immune response. The MAP oral challenge rabbit model was used to evaluate and compare an experimental inactivated MAP vaccine through oral (VOR) and intradermal (VID) routes. The VID group presented the highest proportion of animals with no visible lesions and the lowest proportion of animals with MAP positive tissues. Immunohistochemistry analysis revealed that the VID group presented a dominantly M1 polarized response indicating an ability to control MAP infection. In general, all vaccinated groups showed lower calprotectin levels compared to the non-vaccinated challenged group suggesting less active granulomatous lesions. The VID group showed some degree of skin test reactivity, whereas the same vaccine through oral administration was completely negative. These data show that PTB vaccination has an effect on macrophage polarization and that the route influences infection outcome and can also have an impact on bTB diagnosis. Future evaluation of new immunological products against mycobacterial diseases should consider assaying different vaccination routes.

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Immunohistochemical expression of interferon-γ in different types of granulomatous lesions associated with bovine paratuberculosis

Cited by 10 publications

References 38 publications

Influence of Heterologous and Homologous Vaccines, and Their Components, on the Host Immune Response and Protection Against Experimental Caprine Paratuberculosis

Influence of Heterologous and Homologous Vaccines, and Their Components, on the Host Immune Response and Protection Against Experimental Caprine Paratuberculosis

Methods in isolation and characterization of bovine monocytes and macrophages

Alternative Vaccination Routes against Paratuberculosis Modulate Local Immune Response and Interference with Tuberculosis Diagnosis in Laboratory Animal Models

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