Immunohistochemical Expression of Ki67 and HER2 in Colorectal Cancer Compared to Adenomatous and Normal Samples

Heidari, Zahra; Mahmoudzadeh‐Sagheb, Hamidreza; Jahantigh, Mehdi; Gorgich, Enam Alhagh Charkhat

doi:10.5812/ijcm.12252

Cited by 13 publications

(7 citation statements)

References 43 publications

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“…We found a high statistical significant difference of HER2 expression in CRC group compared with other studied groups which were all negative. This goes with results of Heidari et al, (2017) who found a higher significant difference comparing CRCs to adenomas and normal tissues. Also, Pazurek et al, (2009) found lower expression of HER2 in colon adenoma compared with cancer group by using PCR method.…”

Section: Discussionsupporting

confidence: 91%

Differential Expression of HER2 and SKP2 in Benign and Malignant Colorectal Lesions

Moussa

Badawy

Helal

et al. 2020

Asian Pac J Cancer Prev

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with younger cases usually complicate polyposis and ulcerative colitis (El-Bolkainy et al., 2013). The outcome of CRC patients has significantly improved over the past decades, reflecting continuous progress in understanding its biology, epidemiology, prevention, early diagnosis and treatment (Siegel et al., 2017), but the identification of clinically actionable oncogenic drivers and related predicted biomarkers are largely elusive (Zhang et al., 2020). CRC is a tumor that develops from the progression of acquired or hereditary premalignant lesions. It arises from interactions among different risk factors (environmental,

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Section: Discussionsupporting

confidence: 91%

Differential Expression of HER2 and SKP2 in Benign and Malignant Colorectal Lesions

Moussa

Badawy

Helal

et al. 2020

Asian Pac J Cancer Prev

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“…Крiм того, експресiя Ki-67 у CRC, аденомах та iнтактнiй слизовiй кишкiвника значно вiдрiзняються (79,3%, 44,8%, 25% вiдповiдно), що робить цей маркер привабливим кандидатом для прогностичних висновкiв та скринiнгових дослiджень [23].…”

Section: вступunclassified

Molecular and genetic features of colorectal carcinoma: pathomorphological demonstration of clinical cases and literature review

Shponka¹,

Bondarenko²,

Молокова³

2021

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Background. Colorectal carcinoma (CRC) is one of the most widespread malignancies worldwide; its morbidity rate is on the third place amidst the most common cancers. According to present data, such molecular markers as CyD1, HIF1α, LC3B, p21, p53 as well as the detection of KRAS gene amplification could be used for evaluation of tumor grade, its invasiveness, risk of metastases, sensitivity to anti-EGFR treatment, and further prognosis for patient’s survive. Objective. The purpose of this study was to make a literature review of the CRC molecular diagnostic approaches and demonstrate 5 cases of colorectal carcinoma in patients of Dnipro-city region in purpose to reveal their molecular-genetic features. Methods. Five formalin fixed paraffin embedded specimens of colorectal carcinoma from patients of Dnipro-city region were evaluated pathomorphologically with histological, immunohistochemical and fluorescence in situ hybridization methods. Results. Case study revealed increased level of p53, p21, and LC3B expression, minor elevation of CyD1 and HIF1α expression in demonstrated samples. Amplification of KRAS gene was not found. Conclusion. Our data analysis had revealed multiple studies dedicated to CRC molecular diagnostics with controversial results, what could be explained by both variable methodological approaches and epidemiological peculiarities of CRC in different sites of the globe. Therefore, there is a necessity in empirical identification of the molecular-genetic features of colorectal carcinomas in patients of our region that could improve current state of the art in CRC diagnostic and treatment approaches. Achieved data are the result of the trial and our research of this issue is ongoing.

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“…In human medicine, published data describing Ki-67 expression in normal tissue is scarce. Ki-67 expression was 1.28 ± 0.008% in normal human colon biopsies and <1% in human CD4 T-cells (8,27). So far, no reference data describing the Ki-67 expression in lymphocytes from normal canine lymph nodes are available, neither in histopathology nor in FCM.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes

et al. 2021

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Recent literature suggests conventional flow cytometric (FCM) immunophenotyping complemented by Ki-67 FCM assessment as a reliable tool to classify canine lymphomas. Ki-67 expression assessed by FCM is rarely reported in canine lymphoma cases and reference data for normal canine lymph nodes are missing. Moreover, nothing is known about the Ki-67 expression within the occasionally observed remnant cell population within the gates of normal lymphocytes in lymphoma cases. Aim of this study was to compare flow cytometric Ki-67 expression of lymphocyte populations from normal canine lymph nodes, lymphoma cells from World-Health-Organisation (WHO) classified lymphoma patient samples and their neighboring normal remnant cell population. Cryopreserved lymphocyte cell suspensions from normal lymph nodes from eight dogs free of lymphoma served as reference material. Fourteen cases diagnosed by cytology, FCM, clonality testing, histopathology including immunohistochemistry consisting of 10 DLBCL, 1 MZL, 1 PTCL and 2 TZL showed a residual small lymphocyte population and were investigated. The Ki-67 expression in normal canine lymphoid tissue was 3.19 ± 2.17%. Mean Ki-67 expression in the malignant cell populations was 41 ± 24.36%. Ki-67 positivity was 12.34 ± 10.68% in the residual physiologic lymphocyte population, which otherwise exhibited a physiologic immunophenotype pattern. This ratio was equivalent (n = 3) or lower (n = 11) than the Ki-67 expression of the malignant cell population within the sample. This is the first report of FCM derived Ki-67 expression combined with immunophenotype patterns in normal canine lymph nodes, compared with lymphoma cell populations and residual normal cell populations of lymphoma cases diagnosed by state of the art technology.

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Immunohistochemical Expression of Ki67 and HER2 in Colorectal Cancer Compared to Adenomatous and Normal Samples

Cited by 13 publications

References 43 publications

Differential Expression of HER2 and SKP2 in Benign and Malignant Colorectal Lesions

Differential Expression of HER2 and SKP2 in Benign and Malignant Colorectal Lesions

Molecular and genetic features of colorectal carcinoma: pathomorphological demonstration of clinical cases and literature review

Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes

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