Immunohistochemical expression of non-collagenous extracellular matrix molecules involved in tertiary dentinogenesis following direct pulp capping: a systematic review

Călin, Claudiu; Sajin, M.; Moldovan, Valentin Tiberiu; Coman, Cristin; Stratul, Ştefan‐Ioan; Didilescu, Andreea Cristiana

doi:10.1016/j.aanat.2020.151674

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…DMP 1 is secreted by fully differentiated odontoblasts and plays a fundamental role in dentinogenesis, mediating the mineralization of peritubular dentin and collagen fibrils from intertubular dentin [48,[51][52][53]. Unlike previous investigations in which DMP 1 was expressed in response to calcium hydroxide and MTA [48,54], in the present study, DMP 1 was detected only in 21-day samples treated with pAsp, suggesting that only in this group were the odontoblast-like cells functionally mature.…”

Section: Discussioncontrasting

confidence: 75%

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

dos Santos,

Habelitz,

Nascimento

et al. 2023

JFB

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In recent years, alternative pulpal therapies targeting dentinogenesis signaling pathways using different peptides have been investigated. The aim of this study was to verify the effectiveness of poly(aspartic acid), pAsp, in dentin regeneration using an animal model. Methods: Mechanical pulp exposure was performed in the upper molars of 56 Wistar rats, randomly divided as follows (n = 14): control (no treatment); MTA group—pulp capping with mineral trioxide aggregate (MTA Angelus); pAsp group—application of 20 μL of pAsp solution (25 mg·mL−1); MTA+pAsp group—application of MTA mixed with pAsp (5:1 by mass). Animals were euthanized after 7 or 21 days. Histological sections were submitted to hematoxylin-eosin and Brown and Brenn staining and immunohistochemical analysis for osteopontin (OPN) and dentin matrix protein 1 (DMP 1). Results: At 7 days, an acute inflammatory infiltrate and the presence of disorganized mineralized tissue were observed in all groups. At 21 days, the quality and thickness of the reparative dentin in treated groups were superior to the control, and bacterial contamination was observed in two MTA-pAsp specimens. While all treated groups showed intense immunostaining for OPN at 21 days, only the pAsp group expressed DMP 1, indicating the presence of fully differentiated odontoblast-like cells. Conclusion: Poly(aspartic) acid promoted dentin regeneration in rat molars in the absence of an additional calcium source and may be an alternative to MTA as a pulp-capping agent.

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Section: Discussioncontrasting

confidence: 75%

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

dos Santos,

Habelitz,

Nascimento

et al. 2023

JFB

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“…An ideal DPC material should not induce inflammatory pulpal reactions, which may lead to necrosis, and should provide a quality repair dentin at the level of the exposure site [ 118 ]. Calcium-silicate-based materials have good efficacy on dental pulp when used as DPC agents [ 119 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

et al. 2021

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Dental pulp vitality is a desideratum for preserving the health and functionality of the tooth. In certain clinical situations that lead to pulp exposure, bioactive agents are used in direct pulp-capping procedures to stimulate the dentin-pulp complex and activate reparative dentinogenesis. Hydraulic calcium-silicate cements, derived from Portland cement, can induce the formation of a new dentin bridge at the interface between the biomaterial and the dental pulp. Odontoblasts are molecularly activated, and, if necessary, undifferentiated stem cells in the dental pulp can differentiate into odontoblasts. An extensive review of literature was conducted on MedLine/PubMed database to evaluate the histological outcomes of direct pulp capping with hydraulic calcium-silicate cements performed on animal models. Overall, irrespective of their physico-chemical properties and the molecular mechanisms involved in pulp healing, the effects of cements on tertiary dentin formation and pulp vitality preservation were positive. Histological examinations showed different degrees of dental pulp inflammatory response and complete/incomplete dentin bridge formation during the pulp healing process at different follow-up periods. Calcium silicate materials have the ability to induce reparative dentinogenesis when applied over exposed pulps, with different behaviors, as related to the animal model used, pulpal inflammatory responses, and quality of dentin bridges.

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“…Regarding the role of DMP1 in reparative dentinogenesis during pulp healing, DMP1 is found to be deposited along the boundary between the primary dentin and the newly formed dentin, and its expression preceded the appearance of nestin-immunoreactive cells (odontoblastic marker), active cell proliferation and new matrix formation, suggesting that DMP1 acts as a trigger of pulp repair [ 240 ].…”

Section: Sibling Familymentioning

confidence: 99%

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

Nikoloudaki

2021

IJMS

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Matricellular proteins (MCPs) are defined as extracellular matrix (ECM) associated proteins that are important regulators and integrators of microenvironmental signals, contributing to the dynamic nature of ECM signalling. There is a growing understanding of the role of matricellular proteins in cellular processes governing tissue development as well as in disease pathogenesis. In this review, the expression and functions of different MP family members (periostin, CCNs, TSPs, SIBLINGs and others) are presented, specifically in relation to craniofacial development and the maintenance of orofacial tissues, including bone, gingiva, oral mucosa, palate and the dental pulp. As will be discussed, each MP family member has been shown to have non-redundant roles in development, tissue homeostasis, wound healing, pathology and tumorigenesis of orofacial and dental tissues.

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Immunohistochemical expression of non-collagenous extracellular matrix molecules involved in tertiary dentinogenesis following direct pulp capping: a systematic review

Cited by 8 publications

References 54 publications

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

The Effect of Calcium-Silicate Cements on Reparative Dentinogenesis Following Direct Pulp Capping on Animal Models

Functions of Matricellular Proteins in Dental Tissues and Their Emerging Roles in Orofacial Tissue Development, Maintenance, and Disease

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