Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions

Abstract

VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P < 0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P = 0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P = 0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression.

Decreased cytokeratin 7 expression correlates with the progression of cervical squamous cell carcinoma and poor patient outcomes

“…Belfort‐Mattos et al . reported that podoplanin expression was negative or limited in the basal area of CIN3 lesions . Low podoplanin expression in tumor cells was correlated with the presence of lymphatic invasion and lymph node metastasis .…”

Expression of CK7 and CDKN2 in Cervical Intraepithelial Neoplasia and Correlation with Clinical Outcome

“…Selected samples included 147 cervical biopsies (100 with squamous intraepithelial neoplasia and 47 without neoplasia) which were collected at the Gynecological Disease Prevention Nucleus. Patients were diagnosed by colposcopical and previous histopathological studies, and the absence of neoplasia was confirmed through the cytological and histopathological examination (13). Women with any kind of immunosuppression, diabetes, organ transplantation, or pregnancy were excluded from the study.…”

“…Conventional histopathological method. Routinely, we prepared the formalin-fixed paraffin-embedded tissue blocks (13), which were cut into 4-μm-thick sections using an AO American optical 820 Rotary microtome (AO Instrument Company, NY, USA). After assembly onto a glass slide, the tissue was deparaffinized in xylene, rehydrated in graded alcohols, and submitted to hematoxylin and eosin (HE) staining followed by sealing with Entellan ® (Merck Millipore, Darmstadt, Germany).…”

“…Another potential biomarker for HR-HPV-associated lesions is cytokeratin 7 (CK7) protein, which is synthesized by the KRT7 gene located at chromosome 12q13. 13. It is highly expressed in the squamocolumnar junction (SCJ) of the cervix (8).…”

Angiotensin converting enzyme: the antigenic properties of the domain, role in alzheimer's disease and tumor progression

“…Избыточная экспрессия VEGF наблюдается в большинстве опухолей [56]. Имеются данные, что уровень VEGF коррелирует с клиническими и патологическими особенностями опухолевой прогрессии при карциноме шейки матки [57][58][59]. Показано, что VEGF-сигналинг, индуцируемый AT1R, является одним из ключевых регуляторов роста опухоли и опухолевого ангиогенеза не только в опухолевых тканях, но также в тканях организма-хозяина [60].…”