Immunohistochemical Identification of Epithelial Membrane Antigen in Sweat Gland Tumours by the Use of a Monoclonal Antibody

Noda, Yoko; Horike, H.; Watanabe, Yoshihisa; Mori, M; Terauchi, Takashi

doi:10.1016/s0344-0338(87)80007-9

Cited by 23 publications

(5 citation statements)

References 13 publications

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“…We found no evidence of myoid differentiation in our case, but bmyoidQ apocrine cells were seen, which did not stain with any muscle markers, but did stain with AR and BRST-2. AR and BRST-2 are strong markers of apocrine differentiation in other organs and this finding, along with the EMA staining of the luminal surface of these glands, is in keeping with these cells being apocrine in origin [7][8][9]. Sebaceous cells were also seen in our case as single cells or small clusters within squamous islands, and stained with EMA as expected [10].…”

Section: Discussionsupporting

confidence: 86%

Ectopic hamartomatous thymoma: a case demonstrating skin adnexal differentiation with positivity for epithelial membrane antigen, androgen receptors, and BRST-2 by immunohistochemistry

2007

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Section: Discussionsupporting

confidence: 86%

Ectopic hamartomatous thymoma: a case demonstrating skin adnexal differentiation with positivity for epithelial membrane antigen, androgen receptors, and BRST-2 by immunohistochemistry

2007

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“…These findings are consistent with reports that benign tumours derived from eccrine gland structures are positive for EMA (8,9). Studies have found that eccrine poroma is positive for EMA not only on the luminal surface of ductal structures but also in the cytoplasm and on the plasma membrane of tumour cells (8,9).…”

Section: Discussionsupporting

confidence: 92%

“…The outer cells of both the intraepidermal and upper portion of intradermal eccrine ducts contain EMA, as do the luminal surfaces and intercellular canaliculi of eccrine glands (9). These findings are consistent with reports that benign tumours derived from eccrine gland structures are positive for EMA (8,9). Studies have found that eccrine poroma is positive for EMA not only on the luminal surface of ductal structures but also in the cytoplasm and on the plasma membrane of tumour cells (8,9).…”

Section: Discussionsupporting

confidence: 89%

“…This is further supported by evidence that superficial layers of squamous cell carcinoma express EMA (4). In contrast, it appears that EMA expression in the cytoplasm and on the plasma membrane but not luminal surfaces of ductal structures is lost during malignant transformation of eccrine gland structures, as indicated by comparison of EMA staining between eccrine poroma (8), and the present eccrine porocarcinoma. This is in striking contrast to the finding that almost all adenocarcinomas including metastatic lesions from breast, stomach, pancreas and colon are positive for EMA (5).…”

Section: Discussionmentioning

confidence: 77%

“…Adenocarcinomas from various primary sites contain EMA (5). In normal skin, eccrine, apocrine and sebaceous glands are positive for EMA, but squamous epithelium is negative (6,8). The outer cells of both the intraepidermal and upper portion of intradermal eccrine ducts contain EMA, as do the luminal surfaces and intercellular canaliculi of eccrine glands (9).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Eccrine Porocarcinoma with Bowenoid Changes: Epithelial Membrane Antigen is Not a Useful Marker for Malignant Tumours Arising from Eccrine Gland Structures

Obi¹,

Sotoh²,

Yokozeki³

et al. 2004

Acta Dermato-Venereologica

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A case of eccrine porocarcinoma with Bowenoid changes is reported. We compared the results of immunohistochemical staining for epithelial membrane antigen in the present case with results in Bowen's disease to determine whether the presence of epithelial membrane antigen (EMA) enabled us to differentiate between Bowen's disease and eccrine porocarcinoma with Bowenoid changes. Histologically, the present tumour was characterized by atypical clear cells with Bowenoid changes as well as uniform small cells and intradermal nests with ductal structures. The membrane and cytoplasm of uniform small cells and ductal luminal surfaces were positive for EMA. However, the atypical cells with Bowenoid changes were negative for this. In contrast, tumour cells in Bowen's disease were positive for EMA. Although EMA is known to be a useful marker for some benign tumours derived from eccrine ducts, we found it difficult to distinguish eccrine porocarcinoma with Bowenoid changes from Bowen's disease using immunohistochemical staining for EMA.

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Interleukin-1 induces major phenotypic changes in human skin microvascular endothelial cells

et al. 1997

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To determine the role of the pleiotropic cytokine interleukin-1 (IL-1) on the activation of endothelial cells during inflammation and angiogenesis, pure populations of human dermal microvascular endothelial cells (HDMEC) were obtained by immunoaffinity purification using Ulex europaeus agglutinin-1 and platelet endothelial cell adhesion molecule-1 (PECAM-1) antibody. Exposure of HDMEC to IL-1beta induced morphologic and physiologic changes characterized by 1) phenotypic modulation of endothelial cells from epithelioid to spindle-shaped cells accompanied by reorganization of vimentin filaments; 2) gradual decrease to a complete absence of the endothelial cell markers von Willebrand factor (vWf) and PECAM-1; and 3) increased capability to form tubule-like structures when overlaid with collagen gels. The IL-1 effect on cell morphology, growth, and decrease of endothelial cell antigens was potentiated by basic fibroblast growth factor (bFGF). Similar results were observed in mitotically arrested gamma-irradiated cells demonstrating that the spindle-shaped cells observed after IL-1 stimulation were derived from epithelioid endothelial cells and that DNA synthesis was not required to effect these changes. Immunostaining with an antibody specific for human fibroblasts was negative and further confirmed the endothelial cell origin of the spindle-shaped cells. These data demonstrate that IL-1 can induce phenotypic changes in HDMEC from epithelioid to spindle-shaped, mesenchymal-like cells, that these cells are more susceptible to stimulation by bFGF, and that they lose biochemical and functional properties characteristic of epithelioid HDMEC.

show abstract

Immunohistochemical Identification of Epithelial Membrane Antigen in Sweat Gland Tumours by the Use of a Monoclonal Antibody

Cited by 23 publications

References 13 publications

Ectopic hamartomatous thymoma: a case demonstrating skin adnexal differentiation with positivity for epithelial membrane antigen, androgen receptors, and BRST-2 by immunohistochemistry

Ectopic hamartomatous thymoma: a case demonstrating skin adnexal differentiation with positivity for epithelial membrane antigen, androgen receptors, and BRST-2 by immunohistochemistry

Eccrine Porocarcinoma with Bowenoid Changes: Epithelial Membrane Antigen is Not a Useful Marker for Malignant Tumours Arising from Eccrine Gland Structures

Interleukin-1 induces major phenotypic changes in human skin microvascular endothelial cells

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