Immunohistochemical Identification of HSP47 and Type I Procollagen-expressing Cells in Mongolian Gerbil Salivary Glands: Correlation with Age-related Fibrotic Changes

Tomisaki, Harumi; Sekine, Joji; Inokuchi, Tsugio; Izumi, Shinichi; Takano, Kunio

doi:10.3353/omp.9.141

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…HSP47, known as a collagen specific stress protein, is a molecular chaperone during the processing and/or secretion of procollagen (13,14). HSP47 is an essential protein for the synthesis and processing of various types of collagen during normal development and/or wound healing (13)(14)(15)(16)(17), althought it is involved in the progression of pathologic fibrosis accompanied with surplus production of collagen (15,(18)(19)(20)(21). In this context, we examined the expression of HSP47 to evaluate collagen formation in the healing process of the periosteal defect.…”

Section: Discussionmentioning

confidence: 99%

HSP 47 and Angiogenic Factor Expression and Its Implication for the Healing of Periosteal Defects in the Mouse Cranium

et al. 2006

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We investigated collagen formation during healing in an experimentally-created periosteal defect site (PDS) in terms of the immunohistochemical detection of Heat Shock Protein 47 (HSP47) and-Smooth Muscle Actin (-SMA) along with the histopathological observation. In addition, we investigated expression of angiogenic factors, Vascular Endothelial Growth Factor (VEGF) and von Willebrand Factor (vWF), from the viewpoint of nutritional compensation for the bone in the PDS. The histological findings showed that the PDS was filled with fibrin-like structure until 3 days postoperatively, with granulation tissue at 5 days postoperatively, and it was finally replaced by collagenous fibers. The reparative periosteum-like tissue at 84 days postoperatively, showed features similar to those of normal periosteum. HSP47 and-SMA showed a positive reaction in fibroblast-like cells as well as immature collagen fibers in the healing process of the PDS. At 84 days after operation, some of the fibroblast-like cells in the reparative periosteum-like tissue showed a positive reaction to HSP47 and-SMA, similar to normal periosteum. Some collagen fibers in the normal periosteum showed a positive reaction for vWF. Fibrin-like structure, granulation tissue including fibrobast-like cells and some collagen fibers in the PDS were positive for VEGF and vWF during the experimental term. In conclusion, it is suggested that periosteal defects heal with regeneration of the collagenous structure, which has a role of nourishing the corresponding bone tissue.

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Section: Discussionmentioning

confidence: 99%

HSP 47 and Angiogenic Factor Expression and Its Implication for the Healing of Periosteal Defects in the Mouse Cranium

et al. 2006

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“…Heat shock proteins (HSPs), for instance found in rodent saliva [ 52 , 53 ], can modify functions and density of other proteins, and thus play an important role in numerous physiological processes. In general, they serve as house-keeping proteins in the cell, but are also involved in immunity.…”

Section: Discussionmentioning

confidence: 99%

“…Although many proteins related to stress response have been detected in saliva of nonvenomous mammals [ 51 – 53 ], it is intriguing that so many putative proteins linked to stress and cellular stress response are present in S. araneus venom, and to a lesser extent in N. fodiens venom. On the one hand, high energy demands and the fast pace of shrew life may lead to stress responses [ 34 – 37 ] and thus require efficient mechanisms to minimize stress.…”

Section: Discussionmentioning

confidence: 99%

Proteins from shrews’ venom glands play a role in gland functioning and venom production

Kowalski,

Marciniak,

Nekaris

et al. 2024

Zoological Lett

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Venom production has evolved independently many times in the animal kingdom, although it is rare among mammals. Venomous shrews produce venom in their submandibular salivary glands and use it for food acquisition. Only a few toxins have been identified in shrew venoms thus far, and their modes of action require investigation. The biological and molecular processes relating to venom production and gland functioning also remain unknown. To address this gap, we investigated protein content in extracts from venom glands of two shrew species, Neomys fodiens and Sorex araneus, and interpreted their biological functions. Applying a proteomic approach coupled with Gene Ontology enrichment analysis, we identified 313 and 187 putative proteins in venom glands of N. fodiens and S. araneus, respectively. A search of the UniProt database revealed that most of the proteins found in both shrew species were involved in metabolic processes and stress response, while GO enrichment analysis revealed more stress-related proteins in the glands of S. araneus. Molecules that regulate molecule synthesis, cell cycles, and cell divisions are necessary to enable venom regeneration and ensure its effectiveness in predation and food hoarding. The presence of proteins involved in stress response may be the result of shrews’ high metabolic rate and the costs of venom replenishment. Some proteins are likely to promote toxin spreading during envenomation and, due to their proteolytic action, reinforce venom toxicity. Finally, finding numerous proteins involved in immune response suggests a potential role of shrew venom gland secretions in protection against pathogens. These findings open up new perspectives for studying biological functions of molecules from shrew venom glands and extend our knowledge on the functioning of eulipotyphlan venom systems. Because the majority of existing and putative venomous mammals use oral venom systems to inject venom into target species, the methods presented here provide a promising avenue for confirming or discovering new taxa of venomous mammals.

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Extracellular matrix turnover in salivary gland disorders and regenerative therapies: Obstacles and opportunities

Marinkovic,

Tran,

Wang

et al. 2023

Journal of Oral Biology and Craniofacial Research

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Immunohistochemical Identification of HSP47 and Type I Procollagen-expressing Cells in Mongolian Gerbil Salivary Glands: Correlation with Age-related Fibrotic Changes

Abstract: Tomisaki H, Sekine J, Inokuchi T, Izumi S and Takano K. Immunohistochemical identification of HSP47 and type I procollagen-expressing cells in mongolian gerbil salivary glands: correlation with age-related fibrotic changes.

Cited by 3 publications

References 23 publications

HSP 47 and Angiogenic Factor Expression and Its Implication for the Healing of Periosteal Defects in the Mouse Cranium

HSP 47 and Angiogenic Factor Expression and Its Implication for the Healing of Periosteal Defects in the Mouse Cranium

Proteins from shrews’ venom glands play a role in gland functioning and venom production

Extracellular matrix turnover in salivary gland disorders and regenerative therapies: Obstacles and opportunities

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