Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus

Kawata, Mitsuhiro; Hashimoto, Kozo; Takahara, Jiro; Sano, Yutaka

doi:10.1007/bf00213802

Cited by 37 publications

(14 citation statements)

References 31 publications

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“…GANN et al, 1978) and that the increases of plasma ACTH in the peripheral blood and of CRF in the hypophysial portal blood after hemorrhage are abolished by PVN lesion (PLOTSKY and VALE, 1984). Immunocytochemical studies have shown that cell bodies of CRF-producing neurons exist in the PVN (BLOOM et al, 1982;BUGNON et al, 1982a, b;MERCHENTHALER et al, 1982MERCHENTHALER et al, , 1984OLSCHOWKA et al, 1982;PAULL et al, 1982;VIGH et al, 1982;KAWATA et al, 1983;SWANSON et al, 1983). The present experiments have disclosed that excitability of certain PC cells identified in the PVN increases during hypovolemia due to hemorrhage and resumed the previous level after retransfusion of the shed blood.…”

Section: Conveging Synaptic Inputssupporting

confidence: 51%

Parvocellular neurosecretory neurons : Converging inputs after saphenous nerve and hypovolemic stimulations in the rat.

1986

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Effects of saphenous nerve stimulation and hemorrhage on discharge activity of parvocellular neurosecretory (PC) cells of the paraventricular hypothalamic nucleus (PVN) were studied in anesthetized rats. The PC cells were identified in the PVN with the criteria that units recorded in the PVN showed an antidromically conducted spike after median eminence but not after posterior pituitary stimulation of 0.45 mA intensity and were demonstrated histologically to be located in the parvocellular regions of the PVN. Saphenous nerve stimulation at 4 Hz repetition and of 3 mA intensity increased the discharge rate of 13 of the 52 PC cells tested. These excited cells exhibited a synaptically mediated excitation during post-stimulus period on peristimulus time histograms constructed during 1 Hz stimulation. Hemorrhage (4 ml/kg b.w.) activated 7 of the 10 PC cells tested which had been excited by saphenous nerve stimulation. Both saphenous nerve stimulation and hemorrhage increased immunoreactive adrenocorticotropic hormone (ACTH) in the plasma in the anesthetized rats. These data support the view that synergistic potentiation of ACTH secretion reported to occur after noxious and hypovolemic stimuli depends at least in part on the interaction between afferent neural signals originating from noxious and cardiovascular receptors on their way up to corticotropin-releasing factor (CRF)-secreting PVN neurons.

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Section: Conveging Synaptic Inputssupporting

confidence: 51%

Parvocellular neurosecretory neurons : Converging inputs after saphenous nerve and hypovolemic stimulations in the rat.

1986

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“…1983]. Some CRF-containing cells are also localized in the SON and the anterior hy- pothalamic regions [Merchenthaler et al, 1982;Olschowka et al, 1982;Kawata et al, 1983;Swanson et al, 1983;Bnrlei et al, 1983]. In this study, in the rats treated with colchicine a large number of CRF-containing neurons have been found in the parvocellular part of the PVN, the SON.…”

Section: Discussionmentioning

confidence: 51%

“…especially in its parvocellular part [Bugnon et a!., 1982;Merchenthaler et al, 1982;Olschowka et al, 1983;Antoni et al, 1983;Kawata et al, 1983;Swanson et al. 1983].…”

Section: Discussionmentioning

confidence: 99%

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Immunocytochemical Observations of Corticotropin-Releasing-Factor-Containing Neurons in the Rat Hypothalamus with Special Reference to Neuronal Communication

Shioda¹,

Nakai²,

Kitazawa³

et al. 1985

Cells Tissues Organs

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Synapses between neurons with corticotropin-releasing-factor-(CRF)-like immunoreactivities and other immunonegative neurons in the hypothalamus of colchicine-treated rats, especially in the paraventricular nucleus (PVN) and the supraoptic nucleus (SON) were observed by immunocytochemistry using CRF antiserum. The immunoreactive nerve cell bodies and fibers were numerous in both the PVN and the SON. The CRF-containing neurons had synaptic contacts with immunonegative axon terminals containing a large number of clear synaptic vesicles alone or combined with a few dense-cored vesicles. We also found CRF-like immunoreactive axon terminals making synaptic contacts with other immunonegative neuronal cell bodies and fibers. And since some postsynaptic immunonegative neurons contained many large neurosecretory granules, they are considered to be magnocellular neurosecretory cells. These findings suggest that CRF functions as a neurotransmitter and/or modulator in addition to its function as a hormone.

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“…Such neurons, like in the rat, were present in the parvocellular perikarya of the PVN. However, as reported by Pelletier et al [1982] and Kawata et al [1983a], in the rat hypothalamus. CRFimmunoreactive neurons are also present within the SON.…”

Section: Discussionmentioning

confidence: 76%

Studies on Hypothalamo-Pituitary Corticoliberin System

B¹

1987

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The immunohistochemical localization of CRF- and neurophysin-containing neurons in the hypothalamus of the Mongolian gerbil was studied by means of the PAP technique. The CRF-immunoreactive fibers were detected mainly in the outer layer of the median eminence of intact adult male gerbils. The CRF-positive neurons respond to aminoglutethimide (Elipten, Ciba) administration by showing increased immunoreactivity and an increase in the number of stained cell bodies in the parvocellular division of the paraventricular nucleus. Aminoglutethimide treatment results also in an increase in the number of neurophysin-immunoreactive nervous fibers localized in the internal layer of the median eminence. However, CRF-immunoreactive fibers are observed mainly in the outer layer of the median eminence while neurophysin-immunopositive axons are seen predominantly in the internal layer of this region. Since the axons of paraventricular neurons run to the median eminence and their staining ability is changed due to aminoglutethimide, their involvement in the endocrine control of hypophysial ACTH release is postulated.

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Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus

Cited by 37 publications

References 31 publications

Parvocellular neurosecretory neurons : Converging inputs after saphenous nerve and hypovolemic stimulations in the rat.

Parvocellular neurosecretory neurons : Converging inputs after saphenous nerve and hypovolemic stimulations in the rat.

Immunocytochemical Observations of Corticotropin-Releasing-Factor-Containing Neurons in the Rat Hypothalamus with Special Reference to Neuronal Communication

Studies on Hypothalamo-Pituitary Corticoliberin System

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