Immunohistochemical investigation of biomarkers for predicting adipose tissue invasion in oral squamous cell carcinoma

Han, Yang; Yamada, Shin‐ichi; Kawamoto, Masuki; Gibo, Takahiko; Hashidume, Masao; Otagiri, Hiroki; Tanaka, Hirokazu; Takizawa, Atsushi; Kondo, Eiji; Sakai, Hironori; Uehara, Takeshi; Kitamura, Hiroshi

doi:10.1016/j.ajoms.2021.11.004

Cited by 2 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The worst aggressive pattern of invasion in OSCC tumors exhibited a trend of more frequent mandibular invasion and an infiltrative pattern of invasion ( 3 ). Another study performed immunohistochemistry to identify biomarkers significantly associated with histological adipose tissue invasion in OSCC ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Invasion of the bucco-mandibular space by oral squamous cell carcinoma: histopathological analysis of invasion pattern

Kugimoto,

Nishii,

Oikawa

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

BackgroundThis study aimed to determine the patterns of invasion of oral squamous cell carcinoma (OSCC) into the bucco-mandibular space (BMS) using detailed histopathological analysis and to assess clinical outcomes.MethodsPatients with OSCC who underwent segmental mandibulectomy or hemi-mandibulectomy combined with resection of the BMS between 2012 and 2021 were included. The invasions of the BMS were classified into three patterns. Pattern A was defined as a horizontal invasion, Pattern B as a vertical invasion, and Pattern C as an expansive invasion.ResultsIn total, 109 patients were reviewed. Of these 109 patients, the primary tumor affected the lower gingiva in 78 patients, the buccal mucosa in 18 patients, and was a primary intraosseous carcinoma of the mandible in 13 patients. Invasion of the BMS was significantly associated with a higher pathological T stage, positive/close margins, and lower disease-free survival (DFS) rates. The DFS rates were 86.7% and 66.0% in the BMS non-invasion and invasion groups, respectively. The DFS rates for each type of invasion were 82.1% for Pattern A, 67.4% for Pattern B, and 48.0% for Pattern C (P=0.277).ConclusionPatients with BMS invasion have a poorer prognosis than those without invasion of the BMS. Therefore, adjuvant therapy is necessary, especially in Patterns B and C. Evaluation of preoperative BMS invasion patterns is important for predicting the prognosis of OSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Invasion of the bucco-mandibular space by oral squamous cell carcinoma: histopathological analysis of invasion pattern

Kugimoto,

Nishii,

Oikawa

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

show abstract

“…This result revealed that 36 downregulated DEGs were statistically enriched in the biological process of GO related to EMT, including regulation of cell adhesion, epithelial cell differentiation, and mesenchyme development ( 36 , 37 , 43 ). EMT plays a critical role in tumor progression and metastasis ( 36 – 38 , 44 ). During this process, epithelial cells lose cell-cell adhesion and apicobasal polarity to exhibit migratory mesenchymal properties ( 36 ).…”

Section: Discussionmentioning

confidence: 99%

High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma

et al. 2022

View full text Add to dashboard Cite

The present study aimed to investigate the clinical and biological significance of Src-associated in mitosis 68 kDa (Sam68) in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis was performed on tissue samples obtained from 77 patients with OSCC. Univariate analysis revealed that the high expression of Sam68 was significantly correlated with advanced pathological T stage (P=0.01), positive lymphovascular invasion (P=0.01), and pathological cervical lymph node metastasis (P<0.01).Moreover, multivariate analysis demonstrated that the high expression of Sam68 was an independent predictive factor for cervical lymph node metastasis (odds ratio, 4.39; 95% confidence interval, 1.49-14.23; P<0.01). These results indicated that high Sam68 expression contributed to tumor progression, especially cervical lymph node metastasis, in OSCC. mRNA sequencing was also performed to assess the changes in the transcriptome between OSCC cells with Sam68 knockdown and control cells with the aim of elucidating the biological roles of Sam68. Gene Ontology enrichment analysis revealed that downregulated differentially expressed genes (DEGs) were concentrated in some biological processes related to epithelial-mesenchymal transition. Among these DEGs, it was established that vimentin was particularly downregulated in these cells. It was also confirmed that Sam68 knockdown reduced the motility of OSCC cells. Furthermore, the immunohistochemical study of vimentin identified the association between vimentin expression and Sam68 expression as well as cervical lymph node metastasis. In conclusion, the present study suggested that the high expression of Sam68 may contribute to metastasis by regulating vimentin expression and a motile mesenchymal phenotype in OSCC.

show abstract

Immunohistochemical investigation of biomarkers for predicting adipose tissue invasion in oral squamous cell carcinoma

Cited by 2 publications

References 38 publications

Invasion of the bucco-mandibular space by oral squamous cell carcinoma: histopathological analysis of invasion pattern

Invasion of the bucco-mandibular space by oral squamous cell carcinoma: histopathological analysis of invasion pattern

High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma

Contact Info

Product

Resources

About