Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound‑guided needle biopsy specimens of prostate adenocarcinoma

Singh, Jagtar; Thachil, Thanuja; Eapen, M; Lim, Aijye; Sufyan, Wajiha; Rawson, Robert V.; Duncan, H. J.; Ieso, Paolo De; Sohal, Sukhwinder Singh

doi:10.3892/mco.2021.2353

Cited by 4 publications

(1 citation statement)

References 41 publications

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“…Proinflammatory cytokines IL-6 and TNF-α were evaluated in blinded fashion by clinical pathology consultants. Expression levels were estimated using a semiquantitative scale of grade I (signal intensity) and grade II (cell expansion based on the proportion of positive stain cells) [ 23 ] (see scoring scheme in Supplementary Material Table S1 ). Two grades were added, for a level range of 0–4 used to assess the expression of IL-6 or TNF-α.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Mucosal Barrier Improvement with Prebiotics: Histological Evaluation of Longish Glucomannan Hydrolysates-Induced Innate T Lymphocyte Activities in Mice

et al. 2022

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Use of prebiotics is a growing topic in healthcare. A lightweight molecule and water-soluble fiber ingredient, longish glucomannan hydrolysates (LGH), has been developed to improve the intestinal mucosal barrier and confer gut health benefits. This study aims to investigate the implications of continuous LGH intervening in intestinal epithelium integrity and protective immunity against chemical dextran sodium sulfate (DSS)-induced colitis. Twelve male BALB/c mice were randomly arranged into four groups. The LGH/DSS group had results in bodyweight variance, epithelial cell density, and aberrancy score as good as the LGH group, and both were equivalent to the control group. LGH consumption effectively protects the distal intestinal epithelium by activating innate T lymphocytes. Meanwhile, T-cell subsets in subepithelial interspersion take a bystander role in these microenvironmental alterations. Under this stress, the cluster of differentiation 3 (CD3)+ T cells infiltrate the epithelium, while CD4+ T cells inversely appear in submucosal large lymphoid aggregates/isolated lymphoid follicles (ILFs) in which significant CD3+, CD4+, and CD8+ T-cell populations agglomerate. Moreover, forkhead box P3 (Foxp3) and interleukin 17 (IL-17) are observed in these ILFs. Agglomerated CD4+ T-cell lineages may have roles with proinflammatory T helper 17 cells and anti-inflammatory regulatory T cells in balancing responses to intraluminal antigens. Collectively, LGH administration may function in immune modulation to protect against DSS-induced inflammation.

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Section: Methodsmentioning

confidence: 99%

Intestinal Mucosal Barrier Improvement with Prebiotics: Histological Evaluation of Longish Glucomannan Hydrolysates-Induced Innate T Lymphocyte Activities in Mice

et al. 2022

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Association between endometrial senescent cells and immune cells in women with repeated implantation failure

Parvanov¹,

Ganeva²,

Arsov³

et al. 2023

J Assist Reprod Genet

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Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer

Pecci

Troisi

Aiello

et al. 2023

Preprint

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Background About 30% of Prostate cancer (PCa) patients progress to metastatic PCa that remains largely incurable. This evidence underlines the need for the development of innovative therapies. In this direction, the potential research focus might be on long non-coding RNAs (lncRNAs) like H19, which serve critical biological functions and show significant dysregulation in cancer. Previously, it has been shown that the long noncoding RNA H19 acts as transcriptional repressor of cell adhesion molecules affecting the PCa metastatic potential. Here, we investigated the role of H19/cell adhesion molecules circuitry on in vivo PCa experimental tumor growth and metastatic dissemination models. Methods H19 was silenced in luciferase-positive PC-3 and 22Rv1 cells and in vitro effect was evaluated by gene expression, proliferation, invasion and colony formation assays before and after treatment with the histone lysine demethylase inhibitor, GSK-J4. In vivo tumor growth and metastasis dissemination, in the presence or absence of GSK-J4, were analyzed in two models of human tumor in immunodeficient mice by in vivo bioluminescent imaging and immunohistochemistry (IHC) on explanted tissues. Organotypic Slice Cultures (OSCs) from fresh PCa-explant were used as ex vivo model to test GSK-J4 effects. Analysis of H19/cell adhesion molecules circuitry on PCa biopsies was conducted by IHC and droplet digital PCR on Formalin-Fixed Paraffin-Embedded tissues. Results H19 silencing in both PC-3 and 22Rv1 cells increased: i) E-cadherin and β4 integrin expression as well as invasion and colony formation, ii) in vivo tumor growth, and iii) metastasis formation at bone, lung, and liver. Of note, treatment with GSK-J4 reduced lesions. In parallel, GSK-J4 efficiently induced cell death in PCa-derived OSCs. Lastly, we investigated the potential of early predicting disease progression of the H19/cell adhesion molecules circuitry evaluated at the time of biopsy. Conclusions Our findings underscore the potential of the H19/cell adhesion molecules circuitry as a targeted approach in PCa treatment. Modulating this interaction has proven effective in inhibiting tumor growth and metastasis, presenting a logical foundation for targeted therapy. Additionally, the predictive potential of the H19/cell adhesion molecules circuitry suggests the possibility of an early application in PCa management.

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Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound‑guided needle biopsy specimens of prostate adenocarcinoma

Cited by 4 publications

References 41 publications

Intestinal Mucosal Barrier Improvement with Prebiotics: Histological Evaluation of Longish Glucomannan Hydrolysates-Induced Innate T Lymphocyte Activities in Mice

Intestinal Mucosal Barrier Improvement with Prebiotics: Histological Evaluation of Longish Glucomannan Hydrolysates-Induced Innate T Lymphocyte Activities in Mice

Association between endometrial senescent cells and immune cells in women with repeated implantation failure

Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer

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