Immunohistochemical localisation of 3?-hydroxysteroid dehydrogenase in Sarcocystis spp.

Yarım, Murat; Yıldız, Kader; Kabakci, N.; Karahan, Siyami

doi:10.1007/s00436-004-1152-2

Cited by 5 publications

(5 citation statements)

References 16 publications

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“…For instance, sterol-sterifying enzymes were found in Toxoplasma gondee , a protozoan incapable of cholesterol de novo synthesis (Lige et al, 2013 ). The presence of 3β-hydroxysteroid dehydrogenase in Sarcocystis spp had been shown by immunohistochemistry (Yarim et al, 2004 ); the enzyme is also present in Taenia solium cysticerci and tapeworms (Fernández Presas et al, 2008 ). Genes from the cholesterol synthesis pathway have been found and expressed in Giardia intestinalis (Hernandez and Wasserman, 2006 ).…”

Section: Parasites Express Steroidogenic Enzymesmentioning

confidence: 96%

Parasites and steroid hormones: corticosteroid and sex steroid synthesis, their role in the parasite physiology and development

et al. 2015

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In many cases parasites display highly complex life cycles that include the penetration and permanence of the larva or adults within host organs, but even in those that only have one host, reciprocal, intricate interactions occur. Evidence indicates that steroid hormones have an influence on the development and course of parasitic infections. The host gender's susceptibility to infection, and the related differences in the immune response are good examples of the host-parasite interplay. However, the capacity of these organisms to synthesize their own steroidogenic hormones still has more questions than answers. It is now well-known that many parasites synthesize ecdysteroids, but limited information is available on sex steroid and corticosteroid synthesis. This review intends to summarize some of the existing information in the field. In most, but not all parasitosis the host's hormonal environment determines the susceptibility, the course, and severity of parasite infections. In most cases the infection disturbs the host environment, and activates immune responses that end up affecting the endocrine system. Furthermore, sex steroids and corticosteroids may also directly modify the parasite reproduction and molting. Available information indicates that parasites synthesize some steroid hormones, such as ecdysteroids and sex steroids, and the presence and activity of related enzymes have been demonstrated. More recently, the synthesis of corticosteroid-like compounds has been shown in Taenia solium cysticerci and tapeworms, and in Taenia crassiceps WFU cysticerci. In-depth knowledge of the parasite's endocrine properties will contribute to understand their reproduction and reciprocal interactions with the host, and may also help designing tools to combat the infection in some clinical situations.

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Section: Parasites Express Steroidogenic Enzymesmentioning

confidence: 96%

Parasites and steroid hormones: corticosteroid and sex steroid synthesis, their role in the parasite physiology and development

et al. 2015

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“…Different 3β-HSD isoforms have been cloned from various tissues of humans, rats, and mice (Simard et al 1993;Penning 1997). The immunolocalization of 3β-HSD has been recently shown by Yarim et al (2004) in the parasite Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

The key steroidogenic enzyme 3β-hydroxysteroid dehydrogenase in Taenia solium and Taenia crassiceps (WFU)

et al. 2008

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Larval and adult stages of Taenia solium and Taenia crassiceps WFU strain were analyzed by histochemical and biochemical methods to determine the existence of steroid pathways. The presence of the key enzyme 3beta-hydroxisteroid-dehydrogenase (3beta-HSD) was examined in frozen sections of cysticerci obtained from mice and segments of tapeworms obtained from the intestine of hamsters. 3beta-HSD activity was detected by nitroblue-tetrazolium products after incubation with dehydroepiandrosterone, androstendiol, or pregnenolone. Tapeworm tissues exhibited 3beta-HSD activity in the subtegumentary areas of the neck and immature proglottids following incubation with androstendiol, as well as surrounding the testes in mature proglottids. T. solium cysticerci exhibited 3beta-HSD activity in the subtegumentary tissues. The synthesis of steroid hormones involving the activity of 3beta-HSD was studied in cysticerci or tapeworms incubated in the presence of tritiated steroid precursors. The culture media were analyzed by thin layer chromatography and showed synthesis of androstendiol, testosterone, and 17beta-estradiol by cysticerci, androstendiol, and 17beta-estradiol by tapeworms. The results strongly suggest the activity of 3beta-HSD in taeniid parasites that have at least a part of the enzymatic chain required for androgen and estrogen synthesis and that the enzymes are present in the larval stage and from the early strobilar stages to the mature proglottids.

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“…Interestingly, immunoreactivity for 3␤-hydroxysteroid dehydrogenase/delta 5-4 has been shown in the cytoplasm of the parasite Sarcocystis spp. by immunocytochemistry [41].…”

Section: Discussionmentioning

confidence: 99%

Steroidogenic capacity of Trypanosoma cruzi trypomastigotes

Vacchina

Valdéz

Gómez³

et al. 2008

The Journal of Steroid Biochemistry and Molecular Biology

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Immunohistochemical localisation of 3?-hydroxysteroid dehydrogenase in Sarcocystis spp.

Cited by 5 publications

References 16 publications

Parasites and steroid hormones: corticosteroid and sex steroid synthesis, their role in the parasite physiology and development

Parasites and steroid hormones: corticosteroid and sex steroid synthesis, their role in the parasite physiology and development

The key steroidogenic enzyme 3β-hydroxysteroid dehydrogenase in Taenia solium and Taenia crassiceps (WFU)

Steroidogenic capacity of Trypanosoma cruzi trypomastigotes

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