Immunohistochemical localization and biochemical changes in catalase and superoxide dismutase during metamorphosis in the olfactory system of frog Microhyla ornata

Gaupale, Tekchand C.; Londhe, Jayant; Ghaskadbi, Surendra; Subhedar, Nishikant K.; Bhargava, S.

doi:10.1016/j.neures.2011.11.005

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…Intriguingly, while no effect was observed in DNP-exposed individuals during the larval stage, they showed a decreased metabolic rate in the adult stage. Effects of a stressor on a trait may only become visible in the adult stage as metamorphosis itself is a stressful event (Campero et al, 2008) that may cause, for example, oxidative stress (Gaupale et al, 2012). Together with the DNP exposure in the larval stage this may have caused the adults to reduce their metabolic rate (cfr metabolic depression, Storey, 2015; for damselfly larvae: Van Dinh et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…This illustrates the importance of quantifying synergisms with aquatic pollutants not only during the larval stage, but also during and especially after metamorphosis. Given that the metamorphosis process itself is stressful and metabolically costly (Gaupale et al, 2012; Lowe et al, 2021), this likely left the adults that were food-limited as larvae with not enough energy to protect themselves against the negative influence of DNP. An alternative mechanism through DNP bio-accumulation causing stronger DNP effects later in life seems less likely given the low bio-accumulation potential of DNP (ECHA, 2019; USEPA, 2015).…”

Section: Discussionmentioning

confidence: 99%

Section: The Effects Of Dnp At High Foodmentioning

confidence: 99%

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Synergistic survival-related effects of larval exposure to an aquatic pollutant and food stress get stronger during and especially after metamorphosis and shape fitness of terrestrial adults

Jorissen

Janssens

Verheyen

et al. 2023

Preprint

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To improve the ecological risk assessment of aquatic pollutants it is needed to study their effects not only in the aquatic larval stage, but also in the terrestrial adult stage of the many animals with a complex life cycle. This remains understudied, especially with regard to interactive effects between aquatic pollutants and natural abiotic stressors. We studied effects of exposure to the pesticide DNP (2,4-Dinitrophenol) and how these were modulated by limited food availability in the aquatic larvae, and the possible delayed effects in the terrestrial adults of the damselfly Lestes viridis. Our results revealed that DNP and low food each had large negative effects on the life history, behaviour and to a lesser extent on the physiology of not only the larvae, but also the adults. Food limitation magnified the negative effects of DNP as seen by a strong decline in larval survival, metamorphosis success and adult lifespan. Notably, the synergism between the aquatic pollutant and food limitation for survival-related traits was stronger in the non-exposed adults than in the exposed larvae, likely because metamorphosis is stressful itself. Our results highlight that identifying effects of aquatic pollutants and synergisms with natural abiotic stressors, not only in the aquatic larval but also in the terrestrial adult stage, is crucial to fully assess the ecological impact of aquatic pollutants and to reveal the impact on the receiving terrestrial ecosystem through a changed aquatic-terrestrial subsidy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Effects Of Dnp At High Foodmentioning

confidence: 99%

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Synergistic survival-related effects of larval exposure to an aquatic pollutant and food stress get stronger during and especially after metamorphosis and shape fitness of terrestrial adults

Jorissen

Janssens

Verheyen

et al. 2023

Preprint

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“…Sections were used for the immunohistochemical localization of superoxide dismutase following the standard protocol (Gaupale et al, 2012). Briefly, the sections were deparaffinized, hydrated and then washed with phosphate buffered saline (PBS) thrice and treated with the 0.3% H 2 O 2 in methanol for 45 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…This was mainly suggestive of its protective function against increasing ROS (Starrs et al, 2001). The presence of SOD and its oxidative stress‐protective effect during embryogenesis of mammals, birds, reptiles, fishes and amphibianshas also been described (Frank and Grosecolose, 1984; Gaupale et al, 2012; Khan and Black, 2003; Montesano et al, 1989; Mourente et al, 1999; Qanungo and Mukherjea, 2000; Rizzo et al, 2007; Surai, 1999; Starrs et al, 2001; van Golde et al, 1998). These studies have mostly focused on the biochemical estimation of the enzyme activities during development.…”

Section: Introductionmentioning

confidence: 99%

Localization and distribution of superoxide dismutase‐1 in the neural tube morphogenesis of chick embryo

Dhage¹,

Kamble²,

Bhargava³

2016

Intl J of Devlp Neuroscience

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Superoxide dismutase 1 (SOD- 1) is an antioxidant enzyme that regulates the levels of Reactive oxygen species (ROS) by catalyzing the conversion of superoxide radical into hydrogen peroxide (HO) and oxygen. ROS are known to play a significant role in various cellular processes, via redox modification of a variety of molecules that participate in signaling pathways involved in this processes. As the levels of ROS in cells are controlled by the levels of antioxidant enzymes, thus SOD-1 may be indirectly involved in regulating different cellular processes by maintaining the required levels of HO Therefore, in the present study we have investigated the possible involvement of SOD- 1 in the neurulation during the development of chick embryo. During gastrulation, SOD- 1 immunoreactivity was observed throughout the ectoderm and cauda mesoderm areas, however, its presence during neurulation was restricted to certain areas of neural tube particularly in the dorsal neural tube where neural tube closure takes place. Assaying enzyme activity revealed a significant increase in the SOD activity during neurulation. Further, inhibition of SOD- 1 by Diethyldithiocarbamate (DDC) induced abnormalities in the development of the neural tube. SOD- 1 inhibition specifically affected the closure of neural tube in the anterior region. Thus, here we report the presence of SOD- 1 mainly in the ectoderm and tissues of ectodermal origin during gastrulation to neurulation which suggests that it may be involved in the regulating the cellular processes during neural tube morphogenesis.

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Adaptive and Maladaptive Consequences of Larval Stressors for Metamorphic and Postmetamorphic Traits and Fitness

Stoks

Janssens

Delnat

et al. 2022

Fascinating Life Sciences

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Immunohistochemical localization and biochemical changes in catalase and superoxide dismutase during metamorphosis in the olfactory system of frog Microhyla ornata

Cited by 7 publications

References 37 publications

Synergistic survival-related effects of larval exposure to an aquatic pollutant and food stress get stronger during and especially after metamorphosis and shape fitness of terrestrial adults

Synergistic survival-related effects of larval exposure to an aquatic pollutant and food stress get stronger during and especially after metamorphosis and shape fitness of terrestrial adults

Localization and distribution of superoxide dismutase‐1 in the neural tube morphogenesis of chick embryo

Adaptive and Maladaptive Consequences of Larval Stressors for Metamorphic and Postmetamorphic Traits and Fitness

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