Immunohistochemical localization of acyl-CoA hydrolase/thioesterase multigene family members to rat epithelia

Abstract

Acyl-CoA hydrolases cleave acyl-CoA thioesters to free fatty acids and coenzyme A. The potency of these enzymes may serve to modulate cellular levels of acyl-CoAs to affect various cellular functions, including lipid metabolism. In this study, we investigated the tissue distribution of this multigene family of enzymes, focusing on cytosolic (CTE-I) and mitochondrial acyl-CoA thioesterases (MTE-I) in adult rats, using an anti-CTE-I antibody which recognizes both the isoforms. Western blotting detected them mainly in organs closely related to fatty acid oxidation, of which kidney contained the highest levels of both enzymes. Immunohistochemistry localized the enzymes primarily in the proximal tubules, where a large energy demand is expected and fatty acids represent a major fuel, correlating well with the intrarenal distribution of peroxisomal beta-oxidation. In situ hybridization suggested colocalization of CTE-I and MTE-I in the kidney. The immunoreactivity was also found in various epithelial tissues in the body, including Harderian gland and sebaceous gland. These results demonstrated the distribution of CTE-I and MTE-I in a wide variety of rat tissues, primarily characterized by an epithelial localization, being consistent with their involvement in fatty acid metabolism.

The effect of Acot2 overexpression or downregulation on the preadipocyte differentiation in Chinese Red Steppe cattle

“…The acyl-CoA thioesterase 2 (Acot2), also known as MTE-I, PTE2 and ARTISt/p43 [20], is a member of the acyl-CoA thioesterase (Acots) family [21]. It is highly expressed in the mammalian kidney, heart, liver, brain, brown adipose tissue, skeletal muscles and steroid tissues [23,24,25]. In the rodent model of the diet-induced obesity, and compared with the low-fat diet group, the expression of the Acot2 protein in the heart and soleus muscle of the rats that were fed with high-fat diet increased by 2.0 and 7.6 times, respectively [26].…”