Immunohistochemical localization of alpha and beta adrenergic receptors in the human nasal turbinate

Shirasaki, Hideaki; Kanaizumi, Etsuko; Himi, Tetsuo

doi:10.1016/j.anl.2015.12.002

Cited by 6 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The discrepancies in α-ARs’ involvement in the PTGS-2 expression in response to noradrenaline influence in the inflamed endometrium and earlier reports [ 41 , 47 ] can be related to the experimental model (cells, tissue explants) and kind of tissues. Considering the fact that α-ARs are largely located in vascular cells [ 29 , 50 ], the noradrenaline effect found on the PTGS-2 expression (also mPTGES-1 expression, PGE2 secretion) in the E. coli group endometrial explants could be due to changes in blood flow. It should also be added that downstream mechanisms of AR activation are not fully recognized.…”

Section: Discussionmentioning

confidence: 99%

Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs

Jana

Całka

Bulc

et al. 2023

IJMS

View full text Add to dashboard Cite

In the inflamed uterus, the production and secretion of prostaglandins (PGs) and noradrenergic innervation pattern are changed. Receptor-based control of prostaglandin E2 (PGE2) production and secretion by noradrenaline during uterine inflammation is unknown. The aim of this study was to determine the role of α1-, α2- and β-adrenoreceptors (ARs) in noradrenaline-influenced PG-endoperoxidase synthase-2 (PTGS-2) and microsomal PTGE synthase-1 (mPTGES-1) protein levels in the inflamed pig endometrium, and in the secretion of PGE2 from this tissue. E. coli suspension (E. coli group) or saline (CON group) was injected into the uterine horns. Eight days later, severe acute endometritis developed in the E. coli group. Endometrial explants were incubated with noradrenaline and/or α1-, α2- and β-AR antagonists. In the CON group, noradrenaline did not significantly change PTGS-2 and mPTGES-1 protein expression and increased PGE2 secretion compared to the control values (untreated tissue). In the E. coli group, both enzyme expression and PGE2 release were stimulated by noradrenaline, and these values were higher versus the CON group. The antagonists of α1- and α2-AR isoforms and β-AR subtypes do not significantly alter the noradrenaline effect on PTGS-2 and mPTGES-1 protein levels in the CON group, compared to noradrenaline action alone. In this group, α1A-, α2B- and β2-AR antagonists partly eliminated noradrenaline-stimulated PGE2 release. Compared to the noradrenaline effect alone, α1A-, α1B-, α2A-, α2B-, β1-, β2- and β3-AR antagonists together with noradrenaline reduced PTGS-2 protein expression in the E. coli group. Such effects were also exerted in this group by α1A-, α1D-, α2A-, β2- and β3-AR antagonists with noradrenaline on mPTGES-1 protein levels. In the E. coli group, the antagonists of all isoforms of α1-ARs and subtypes of β-ARs as well as α2A-ARs together with noradrenaline decreased PGE2 secretion versus noradrenaline action alone. Summarizing, in the inflamed pig endometrium, α1(A, B)-, α2(A, B)- and β(1, 2, 3)-ARs mediate the noradrenaline stimulatory effect on PTGE-2 protein expression, while noradrenaline via α1(A, D)-, α2A- and β(2, 3)-ARs increases mPTGES-1 protein expression and α1(A, B, D)-, α2A- and β(1, 2, 3)-ARs are involved in PGE2 release. Data suggest that noradrenaline may indirectly affect the processes regulated by PGE2 by influencing its production. Pharmacological modulation of particular AR isoforms/subtypes can be used to change PGE2 synthesis/secretion to alleviate inflammation and improve uterine function.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs

Jana

Całka

Bulc

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Immunohistochemical analysis was carried out using antihuman adrenergic subtype-specific protein antibodies (α1A-, α1D-, α2C-and β2-adrenergic receptors). The immunostaining displayed that submucosal glands contain a high amount of α1D-and β2-adrenergic receptors while smooth muscle surrounding the vasculature contains a high amount of α1A-and α2C-adrenergic receptors (Shirasaki, Kanaizumi, and Himi 2016).…”

Section: Autonomic Receptorsmentioning

confidence: 99%

“…One example is an α-adrenergic receptor agonist drug that mimics sympathetic agents to increase airflow. They function by binding to adrenergic receptors, which starts a excitatory cascade that results in decreased volume of the nasal mucosa, which decreases resistance and increases airflow (Shirasaki, Kanaizumi, and Himi 2016).…”

Section: Airway Resistancementioning

confidence: 99%

The inferior turbinate: An autonomic organ

Smith

Brook

Virani

et al. 2018

American Journal of Otolaryngology

View full text Add to dashboard Cite

“…ARs are cell membrane receptors included in the seven-transmembrane-spanning G-protein coupled receptor (GPCR) superfamily, formed by membrane proteins triggered by extracellular ligands such as small organic molecules, peptides, and neurotransmitters [15]. Structurally, GPCRs are formed by seven hydrophobic transmembrane segments which cross the plasma membrane with an extracellular amino terminus and an intracellular carboxyl-terminus.…”

Section: Introductionmentioning

confidence: 99%

“…According to their pharmacological response, ARs were subdivided into α and β types: these latter types were further divided into three families called β1, β2 and β3 on the basis of molecular classification [19,20]. β2-ARs have been described in multiple locations such as the lung, heart, blood vessels, and nasal turbinates [13,15,20]. However, there is little data available on the presence of β2-ARs in the adrenal gland.…”

Section: Introductionmentioning

confidence: 99%

Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland

Spagnolo

Mondello

Cardia

et al. 2019

IJMS

View full text Add to dashboard Cite

The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. In conclusion, the authors provide a possible explanation for the observed differences in expression in relation to the cause of death.

show abstract

Immunohistochemical localization of alpha and beta adrenergic receptors in the human nasal turbinate

Cited by 6 publications

References 13 publications

Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs

Role of Noradrenaline and Adrenoreceptors in Regulating Prostaglandin E2 Synthesis Cascade in Inflamed Endometrium of Pigs

The inferior turbinate: An autonomic organ

Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland

Contact Info

Product

Resources

About