Immunohistochemical localization of androgen and progesterone receptors in the uterus of the camel (Camelus dromedarius)

Emam, Mahmoud Abdelghaffar

doi:10.1016/j.acthis.2014.07.004

Cited by 7 publications

(7 citation statements)

References 28 publications

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“…This finding is in accordance with those of Robinson, Mann, Lamming, and Wathes () who stated that in the cow, PR expression was higher in the stroma than it was in the various types of epithelial cells and that increased expression was observed at oestrus and during the early luteal phase. Contradicting our findings, Emam () has mentioned that the cyclic camel uterus exhibited a pronounced expression of progesterone and androgen receptors in both epithelial and stromal components. It is, however, in contrast with results from Spencer and Bazer () in the sheep endometrium, who reported that steroid receptor regulation of oestrogen receptors as well as PR is most pronounced in both luminal and glandular epithelial cells, and is observed to a much smaller extent in stromal cells.…”

Section: Discussionsupporting

confidence: 53%

“…Understanding the expression and localization of progesterone (PR) receptors in the uterus of the dromedary camel is key to understanding the physiological events occurring in the uterus during reproductive cycling as well as during pregnancy. Emam () reported data on progesterone receptors in the cycling dromedary camel but not the pregnant dromedary camel. Thus, the focus of this study was to characterize and compare the distribution of progesterone (PR) receptors in the uterine endometrium and myometrium between nonpregnant (females with newly formed corpora lutea present) based on our observations and those of Emam () and pregnant camels at late pregnancy.…”

Section: Introductionmentioning

confidence: 99%

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Survey of nuclear progesterone receptor expression in the uterus of the cyclic and pregnant camel (Camelus dromedarius)

Osman

Abbott

Ahmed

2018

Anat Histol Embryol

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Progesterone receptors (PR) are necessary to mediate the biological effects of progesterone and are integral to the regulation of a number of different aspects of reproduction in mammals including ovulation of the oocyte, implantation of the conceptus and maintenance of pregnancy. This study investigated the expression and localization of progesterone receptors in the uterine wall of both pregnant and cyclic (nonpregnant) camels. Uterine tissue samples were collected from healthy animals and processed for routine histological and immunohistochemical staining techniques to reveal nuclear PR. Demonstration of PR was performed by indirect immunohistochemical techniques using monoclonal antibodies raised against human PR. Immunolocalization of PR was more intense in all four endometrial zones (I-IV) as well as the myometrium of non pregnant (cyclic) animals (animals with newly formed corpus luteum). In contrast, PR immunostaining in both the endometrium and the myometrium was greatly reduced in pregnancy, particularly in the latest stage examined (approximately 366 days of gestation). In conclusion, a better understanding of the expression of steroid hormones and their receptors, including progesterone and the PR is critical to improving the reproductive health and pregnancy in the domesticated dromedary camel.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Survey of nuclear progesterone receptor expression in the uterus of the cyclic and pregnant camel (Camelus dromedarius)

Osman

Abbott

Ahmed

2018

Anat Histol Embryol

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“…In human (Amso, Crow, & Shaw, 1994), mouse (Couse & Korach, 2001), cattle (Kimmins & Maclaren, 2001), sheep (Meikle, Tasende, Sosa, & Garófalo, 2004), dromedary (Emam, 2014) and pig (Sukjumlong, Persson, Dalin, Janson, & Sahlin, 2009), the ER is found in the uterus. Combined with oestrogen, ER can increase endometrial hyperplasia and glandular secretion (Spencer & Bazer, 2004;Tarleton, Wiley, & Bartol, 1999); this receptor also maintains myometrial contraction (Spencer, Wiley, & Bartol, 1993).…”

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confidence: 99%

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Duan

Xiao

et al. 2019

Reprod Domestic Animals

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Oestrogen, androgen and progesterone are involved in the regulation of uterine physiological functions, with the participation of the following proteins: oestrogen receptor (ER), androgen receptor (AR) and progesterone nuclear receptor (PGR). In this study, we used immunohistochemistry to detect the localization of ERα, ERβ, AR and PGR in sheep uterus. Additionally, we used real‐time polymerase chain reaction (RT‐qPCR) and Western blot technique to analyse their expression profiles at different stages of sheep oestrous cycle in the endometrium and myometrium. Immunohistochemical analysis showed that ERα, ERβ, AR and PGR were present in sheep uterus in oestrus, mainly in the uterine luminal epithelium, stroma, gland and myometrium. Real‐time polymerase chain reaction results showed that in the endometrium, ERα expression level was highest in oestrus. ERβ and PGR, instead, were highly expressed in pro‐oestrus. In the myometrium, ERα was highly expressed in both oestrus and pro‐oestrus, and ERβ was highly expressed in oestrus and dioestrus. Progesterone nuclear receptor expression was highest in oestrus, followed by metoestrus. In the endometrium, both receptors ERα and ERβ were abundant in pro‐oestrus, while the maximum AR protein content was found in oestrus. At this stage of the oestrous cycle, PGR protein concentration in the myometrium was significantly lower than those observed in other stages. These results suggest that these receptors are important for sheep reproductive function, as their expression at mRNA and protein levels exhibits particular time‐ and tissue‐specific profiles along the oestrous cycle.

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“…The pattern of PR expression of A. lituratus resembles that of other mammals, in which PR expression is linked to cells of the stroma, glands, and superficial epithelium of the endometrium and the myometrium (Emam, ; Vermeirsch, Simoens, Hellemans, Coryn, & Lauwersl, ).…”

Section: Discussionmentioning

confidence: 97%

Evaluation of the uterine hormonal control of the bat Artibeus lituratus during the different phases of its reproductive cycle

Santiago

Albernaz

Santos

et al. 2020

Journal of Morphology

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Artibeus lituratus is a frugivorous bat that directly assists in the restoration of degraded habitats through the effective dispersion of seeds and fruits. Given its great importance, this work aimed to evaluate the uterine hormonal control of A. lituratus during its different reproductive phases. The uteri of 30 sexually mature adult females, five specimens for each of the six sample groups (NON, nonreproductive; P1, initial pregnancy; P2, intermediate pregnancy; P3, advanced pregnancy; LAC, lactating; P + LAC, pregnant‐lactating), were submitted to analyses of serum estradiol and progesterone concentrations, in addition to immunohistochemical analyses. Both estradiol and progesterone, gradually increased during pregnancy, with a marked significant increase in P3 females. Both returned to low levels in LAC‐females; however, estradiol levels decreased further in P + LAC‐females, while progesterone increased in the same group. In general, signs indicative of aromatase expression were observed in the endometrium of all analyzed groups and in the placenta of bats in the gestation groups. Similarly, ERα and PR were expressed in the myometrium, endometrium and placenta at varying levels of intensity. The results indicate that the uterine microenvironment of A. lituratus is directly regulated by serum concentrations of estradiol and progesterone, and fluctuations in these concentrations control morphological and physiological changes of this organ during different phases of the reproductive cycle. Research highlights Increases in serum concentrations of estradiol and progesterone coordinate the gestational period of A. lituratus. Estradiol activates ERα, stimulating cell proliferation in the uterus, in addition to activating the expression of PR, which trigger the quiescence of the myometrium and stimulation of the secretion and differentiation of the endometrium. Results showed several similarities to humans, indicating the use of A. lituratus as an animal model in reproductive studies.

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Immunohistochemical localization of androgen and progesterone receptors in the uterus of the camel (Camelus dromedarius)

Cited by 7 publications

References 28 publications

Survey of nuclear progesterone receptor expression in the uterus of the cyclic and pregnant camel (Camelus dromedarius)

Survey of nuclear progesterone receptor expression in the uterus of the cyclic and pregnant camel (Camelus dromedarius)

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Evaluation of the uterine hormonal control of the bat Artibeus lituratus during the different phases of its reproductive cycle

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