Immunohistochemical localization of calcium-binding proteins (CaBPs) in the epidermis of the earthworm Lumbricus terrestris (Annelida, Oligochaeta)

Licata, A.; Mauceri, Angela; Ricca, M. B.; Cascio, Patrizia Lo; Martella, Silvestro; Amato, Aldo

doi:10.1078/s0065-1281(04)70025-2

Cited by 11 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1), and a pseudostratified epithelium in the clitellar region. In all epidermal regions are present supporting, basal, sensory and glandular mucous cells as reported in previous studies (Richards, 1973(Richards, , 1974Licata et al, 1998Licata et al, , 2000. The mucous cells (orthochromatic and metachromatic) are mainly present in the clitellar region, while neuroendocrine-like cells are more numerous in the cephalic and terminal regions.…”

Section: Resultssupporting

confidence: 81%

“…The secretion of these cells could aid water retention in the acidic mucous film or modify the viscosity of the lubricating mucus (Richards, 1974). A receptor-secretory function with paracrine action has also been attributed considering these cells as neuroendocrine-like, capable of producing bioactive substances (Licata et al, 1994(Licata et al, , 1998(Licata et al, , 2000. The aim of this study is to characterize the glycoconjugates of the glandular cells of the Lumbricus terrestris epidermis using a battery of four biotinylated lectins and to correlate their binding to the epidermal cells function.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lectin histochemistry of epidermal glandular cells in the earthworm Lumbricus terrestris (Annelida Oligochaeta)

Licata

Mauceri²,

Ainis³

et al. 2009

Eur J Histochem

View full text Add to dashboard Cite

Carbohydrate residues were localized in the glandular cells of the epidermis of Lumbricus terrestris by lectin histochemistry. The following biotinylated lectins were used: ConA, PNA, WGA, UEA-I. Each lectin has a specific binding pattern in the epidermal glandular cells. The ConA binding is evident in the orthochromatic mucous cells; PNA in the metachromatic mucous cells; WGA in the neuroendocrine-like cells; UEA-I in the cuticle. The epidermal glandular cells possess specific sites for the different lectins in relation to their functional characteristics. Therefore, these sugar residues indicate different behaviours of the cells in epidermal functions related to ion transport, receptor-secretory processes and defence

show abstract

Section: Resultssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Lectin histochemistry of epidermal glandular cells in the earthworm Lumbricus terrestris (Annelida Oligochaeta)

Licata

Mauceri²,

Ainis³

et al. 2009

Eur J Histochem

View full text Add to dashboard Cite

show abstract

“…In the skin of mammals, serotonin has been implicated in enhancing inflammatory reactions by attracting mast cells at the site of tissue lesion (Kushnir‐Sukhov et al,2006), and a similar role for 5‐HT in the dermis of lampreys may occur. The absence of serotonergic structures in the epidermis contrasts with their presence in the epidermis of different invertebrates (Myhrberg,1971; Licata et al,1998; McKenzie et al,1998; Faccioni‐Heuser et al,2004) and vertebrates (Zaccone,1986; García‐Caballero et al,1989; Zaccone et al,1990,1994; Fasulo et al,1993). The presence of serotonin in epidermal Merkel cells has been reported in teleosts, amphibians, dipnoans, and mammals (Whitear,1989; García‐Caballero et al,1989; English et al,1992; Zaccone et al,1994).…”

Section: Discussionmentioning

confidence: 99%

Extensive presence of serotonergic cells and fibers in the peripheral nervous system of lampreys

Barreiro‐Iglesias

Aldegunde

Anadón

et al. 2008

J of Comparative Neurology

View full text Add to dashboard Cite

Lampreys are suitable animal models for studying the evolution of the vertebrate nervous system because they belong to the earliest group of extant vertebrates, the agnathans. The general organization of the serotonergic central nervous system is well known in lampreys, but information about its peripheral organization is lacking. In the present study, high-performance liquid chromatography and immunohistochemistry with an antibody against serotonin were used to study the presence and peripheral distribution of serotonergic elements in the head of larval sea lampreys. Adult lampreys were also analyzed in order to compare the organization of the system in larval and postmetamorphic stages. Serotonergic cells were observed in the cranial nerve ganglia, the musculature, the taste buds, and in the gills. Serotonergic fibers were extensive in all tissues except the epidermis. Possible roles were proposed for the different cell types observed in relation to their location, structure, and innervation patterns. The serotonergic cells appear to be involved in the oxygen and taste chemoreception processes, the modulation of musculature contraction, and in the regulation of cilia beat frequency and mucus secretion in lampreys. The extensive presence of serotonergic elements in the peripheral nervous system of lampreys suggests a close relationship with the organization of this system in invertebrate groups.

show abstract

“…Many studies have been carried out regarding the morphology, physiology, and histochemistry of the epidermal glandular cells of oligochaetes (Richards 1973, 1974a,b; Licata et al 1994, 1998, 2000, 2002). The literature relative to the epidermis of polychaetes is scarce.…”

mentioning

confidence: 99%

Histochemical and ultrastructural analysis of the epidermal gland cells of Branchiomma luctuosum (Polychaeta, Sabellidae)

Mastrodonato

Lepore

Gherardi

et al. 2005

Invertebrate Biology

View full text Add to dashboard Cite

Abstract. This histochemical and ultrastructural study describes the epidermal gland cells of a tubicolous polychaete, Branchiomma luctuosum. The histochemistry was carried out using standard techniques and FITC‐labelled lectins. Four types of secretory cells were identified in two categories: orthochromatic cells (Type 1) and metachromatic cells (Types 2, 3, and 4). The secretory product of the Type‐1 orthochromatic cells contains neutral glycoproteins with Galβ1,3GalNAc residues. Metachromatic cells produce acidic, mainly sulfated, glycoconjugates with Galβ1,3GalNAc residues (Type 2) or glucosidic and/or mannosidic residues (Types 3 and 4). In sialylated chains, terminal sialic acid is bound to the penultimate GalNAc and Galβ1,3GalNAc residues. The complex composition of the mucus produced by epidermal gland cells of B. luctuosum may be correlated with its different functions. Ultrastructural studies of the epidermal gland cells showed differing morphology, and the presence in the gland cells of Types 3 and 4 of a funnel‐shaped structure for the extrusion of the secretory material.

show abstract

Immunohistochemical localization of calcium-binding proteins (CaBPs) in the epidermis of the earthworm Lumbricus terrestris (Annelida, Oligochaeta)

Cited by 11 publications

References 12 publications

Lectin histochemistry of epidermal glandular cells in the earthworm Lumbricus terrestris (Annelida Oligochaeta)

Lectin histochemistry of epidermal glandular cells in the earthworm Lumbricus terrestris (Annelida Oligochaeta)

Extensive presence of serotonergic cells and fibers in the peripheral nervous system of lampreys

Histochemical and ultrastructural analysis of the epidermal gland cells of Branchiomma luctuosum (Polychaeta, Sabellidae)

Contact Info

Product

Resources

About