Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol

Thoungseabyoun, Wipawee; Tachow, Apussara; Pakkarato, Sawetree; Rawangwong, Atsara; Krongyut, Suthankamon; Sakaew, Waraporn; Kondo, Hisatake; Hipkaeo, Wiphawi

doi:10.1016/j.archoralbio.2017.05.003

Cited by 12 publications

(11 citation statements)

References 23 publications

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“…Positive signals for CB1 and CB2 were localized within the cytoplasm of ductal epithelial and serous acinar cells rather than in their cell membranes. Characteristically, however, even if CB receptors are described as membrane receptors (Thoungseabyoun et al., ) where they exert their primary functions, they are also localized in the cytoplasm as a consequence of their shuttle between plasma membranes and cytoplasm (Kleyer et al., ; Leterrier, Bonnard, Carrel, Rossier, & Lenkei, ). The Western blotting analysis confirmed the results of the immunohistochemistry by detecting protein bands corresponding to the expected weights of both receptors.…”

Section: Discussionmentioning

confidence: 99%

“…In all animals belonging to the four experimental groups (a), immunopositivity is localized in the ductal epithelial cells near or on the apical cell membrane (arrow); in the same sections, CB2 immunopositivity is also evident in neuronal ganglia (b, arrows) CB2 were localized within the cytoplasm of ductal epithelial and serous acinar cells rather than in their cell membranes. Characteristically, however, even if CB receptors are described as membrane receptors (Thoungseabyoun et al, 2017) where they exert their primary functions, they are also localized in the cytoplasm as a consequence of their shuttle between plasma membranes and cytoplasm (Kleyer et al, 2012;Leterrier, Bonnard, Carrel, Rossier, & Lenkei, 2004). The…”

Section: Discussionmentioning

confidence: 99%

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The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

Pirino

Cappai

Maranesi

et al. 2017

Animal Physiology Nutrition

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We explored the expression and cell type distribution of cannabinoid receptors type 1 (CB1) and cannabinoid receptors type 2 (CB2) in the mandibular glands of pigs in relation to different physical forms of the diet. Thirty-two crossbred growing pigs (ages 5-6 weeks) were randomly allotted to four experimental groups (eight pigs/group) and fed four different physical types of the same diet for 4 weeks: finely ground pellet (FP), coarsely ground meal (CM), coarsely ground pellet (CP) and coarsely ground extruded (CE) with dMEAN of 0.46, 0.88, 0.84 and 0.66 mm respectively. At the end of the feeding trial, the pigs were euthanized and the mandibular gland was collected after dissection. By immunohistochemistry, positive signals for CB1 were found in the cytoplasm of duct epithelial cells of pigs fed CP, FP and CE diets and in the serous cells of mixed acini in pigs fed the coarser CM diet. Positive signals for CB2 were detected in duct epithelial cells and in neurons of ganglia close to major secretory ducts of all pigs. The differential expression and localization of these receptors in response to variable chewing activity due to the type of diet suggest that endocannabinoids may influence the functional activity of the mandibular gland by modifying qualitative and/or quantitative aspects of salivary secretion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

Pirino

Cappai

Maranesi

et al. 2017

Animal Physiology Nutrition

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“…; Thoungseabyoun et al. ). The three major salivary glands on the left side of each mouse were used individually for western blotting analysis and those on the right side for immuno‐histochemical analysis.…”

Section: Methodsmentioning

confidence: 97%

“…Thirty male mice at postnatal age 8 weeks were divided into two groups: one for analysis under normal conditions (six mice), the other for time-course analyses (six mice each at stages of 15, 30, 60, 120 min post-injection) under extrinsic stimulation by single-shot intraperitoneal injection of isoproterenol (IPR, 15 mg kg -1 bodyweight in physiological saline; Sigma Aldrich, Welwyn Garden City, UK). This amount of IPR has been shown to be appropriate for experimental stimulation Thoungseabyoun et al 2017). The three major salivary glands on the left side of each mouse were used individually for western blotting analysis and those on the right side for immuno-histochemical analysis.…”

Section: Animals and Tissues Preparationmentioning

confidence: 99%

Localization of phosphatidylinositol 4‐phosphate 5‐kinase (PIP5K) α, β, γ in the three major salivary glands in situ of mice and their response to β‐adrenoceptor stimulation

et al. 2019

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Phosphatidylinositol 4‐phosphate 5‐kinase (PIP5K), which is composed of three isozymes (α, β and γ), catalyzes the production of phosphatidylinositol bisphosphate (PIP2). This phospholipid functions in membrane trafficking, as an anchor for actin cytoskeletons and as a regulator of intramembranous channels/transporters. It is also a precursor of such second messengers as diacylglycerol, inositol triphosphate and phosphatidylinositol (3,4,5)‐triphosphate. In the present study, the expression and localization of endogenous PIP5Ks were examined in the three major salivary glands of young adult mice in situ. In western blotting of normal control glands, immunoreactive bands for individual PIP5Ks were detectable, with the highest density in the parotid gland and the weakest density in the submandibular gland. In immuno‐light microscopy under non‐stimulated condition, weak immunoreactivity for PIP5Kα was confined to the apical plasmalemma in parotid, but not sublingual or submandibular, acinar cells. Immunoreactivity for PIP5Kβ was weak to moderate and confined to ductal cells but not acinar cells, whereas that for PIP5Kγ was selectively and intensely detected in myoepithelial cells but not acinar cells, and it was weak in ductal cells in the three glands. In western blot of the parotid gland stimulated by isoproterenol, a β‐adrenoceptor agonist, no changes were seen in the intensity of immunoreactive bands for any of the PIP5Ks. In contrast, in immuno‐light microscopy, the apical immunoreactivity for PIP5Kα in parotid acinar cells was transiently and distinctly increased after the stimulation. The increased immunoreactivity was ultrastructurally localized on most apical microvilli and along contiguous plasma membrane, where membranous invaginations of various shapes and small vesicles were frequently found. It was thus suggested that PIP5Kα is involved in post‐exocytotic membrane dynamics via microvillous membranes. The present finding further suggests that each of the three isoforms of PIP5K functions through its product PIP2 discretely in different cells of the glands to regulate saliva secretion.

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“…Salivary glands express both CB 1 and CB 2 receptors with specific patterns [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ]. CB 1 receptors have been detected in the major salivary glands, however their expression was not observed in the acinous cells but were restricted to the striated duct cells near to the apical membrane [ 87 ].…”

Section: Introductionmentioning

confidence: 99%

Cannabinoids Drugs and Oral Health—From Recreational Side-Effects to Medicinal Purposes: A Systematic Review

Bellocchio

Inchingolo

et al. 2021

IJMS

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Background: marijuana, the common name for cannabis sativa preparations, is one of the most consumed drug all over the world, both at therapeutical and recreational levels. With the legalization of medical uses of cannabis in many countries, and even its recreational use in most of these, the prevalence of marijuana use has markedly risen over the last decade. At the same time, there is also a higher prevalence in the health concerns related to cannabis use and abuse. Thus, it is mandatory for oral healthcare operators to know and deal with the consequences and effects of cannabis use on oral cavity health. This review will briefly summarize the components of cannabis and the endocannabinoid system, as well as the cellular and molecular mechanisms of biological cannabis action in human cells and biologic activities on tissues. We will also look into oropharyngeal tissue expression of cannabinoid receptors, together with a putative association of cannabis to several oral diseases. Therefore, this review will elaborate the basic biology and physiology of cannabinoids in human oral tissues with the aim of providing a better comprehension of the effects of its use and abuse on oral health, in order to include cannabinoid usage into dental patient health records as well as good medicinal practice. Methods: the paper selection was performed by PubMed/Medline and EMBASE electronic databases, and reported according to the PRISMA guidelines. The scientific products were included for qualitative analysis. Results: the paper search screened a total of 276 papers. After the initial screening and the eligibility assessment, a total of 32 articles were considered for the qualitative analysis. Conclusions: today, cannabis consumption has been correlated to a higher risk of gingival and periodontal disease, oral infection and cancer of the oral cavity, while the physico-chemical activity has not been completely clarified. Further investigations are necessary to evaluate a therapeutic efficacy of this class of drugs for the promising treatment of several different diseases of the salivary glands and oral diseases.

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Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol

Cited by 12 publications

References 23 publications

The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

Localization of phosphatidylinositol 4‐phosphate 5‐kinase (PIP5K) α, β, γ in the three major salivary glands in situ of mice and their response to β‐adrenoceptor stimulation

Cannabinoids Drugs and Oral Health—From Recreational Side-Effects to Medicinal Purposes: A Systematic Review

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