Immunohistochemical localization of CB1 receptor in canine salivary glands

Dall’Aglio, Cecilia; Mercati, Francesca; Pascucci, Luisa; Boiti, Cristiano; Pedini, V.; Ceccarelli, Piero

doi:10.1007/s11259-010-9379-0

Cited by 25 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This particular distribution might be due to the fact that the CB 1 receptors are mainly expressed in axons and pre synaptic terminals [36–38], emphasizing the important role of this receptor as a modulator of neurotransmitter release at specific synapses [9, 39, 40]. CB 1 receptors, however, have also been found on postsynaptic structures [22, 23, 41], glial cells [42–45] and peripheral cells such as cells of the striated ducts of the parotid and mandibular glands, keratinocytes, fibroblasts and macrophages [24, 25, 46–48]. …”

Section: Discussionmentioning

confidence: 99%

“…In addition, CB 1 expression has also been described particularly in the dorsal horn in rats [19, 23] and in the spinal cord of humans [17]. In the species dog, CB 1 receptors were detected in salivary glands [24], hair follicles [25], skin and hippocampus [26]. However, a detailed analysis of the distribution of CB 1 receptors in the CNS and PNS has not been reported in canines so far.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system

et al. 2017

View full text Add to dashboard Cite

The endocannabinoid system is a regulatory pathway consisting of two main types of cannabinoid receptors (CB1 and CB2) and their endogenous ligands, the endocannabinoids. The CB1 receptor is highly expressed in the central and peripheral nervous systems (PNS) in mammalians and is involved in neuromodulatory functions. Since endocannabinoids were shown to be elevated in cerebrospinal fluid of epileptic dogs, knowledge about the species specific CB receptor expression in the nervous system is required. Therefore, we assessed the spatial distribution of CB1 receptors in the normal canine CNS and PNS. Immunohistochemistry of several regions of the brain, spinal cord and peripheral nerves from a healthy four-week-old puppy, three six-month-old dogs, and one ten-year-old dog revealed strong dot-like immunoreactivity in the neuropil of the cerebral cortex, Cornu Ammonis (CA) and dentate gyrus of the hippocampus, midbrain, cerebellum, medulla oblongata and grey matter of the spinal cord. Dense CB1 expression was found in fibres of the globus pallidus and substantia nigra surrounding immunonegative neurons. Astrocytes were constantly positive in all examined regions. CB1 labelled neurons and satellite cells of the dorsal root ganglia, and myelinating Schwann cells in the PNS. These results demonstrate for the first time the spatial distribution of CB1 receptors in the healthy canine CNS and PNS. These results can be used as a basis for further studies aiming to elucidate the physiological consequences of this particular anatomical and cellular distribution.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Currently, data regarding the presence and localization of cannabinoid receptors in the major salivary glands of domestic animals are limited to the dog (Dall'Aglio et al., ). Therefore, the aim of this study was to evaluate (i) whether CB1 and CB2 are expressed in the mandibular gland of pigs and (ii) whether different physical types of the same diet modulate their expression in different ways.…”

Section: Introductionmentioning

confidence: 99%

The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

Pirino

Cappai

Maranesi

et al. 2017

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

We explored the expression and cell type distribution of cannabinoid receptors type 1 (CB1) and cannabinoid receptors type 2 (CB2) in the mandibular glands of pigs in relation to different physical forms of the diet. Thirty-two crossbred growing pigs (ages 5-6 weeks) were randomly allotted to four experimental groups (eight pigs/group) and fed four different physical types of the same diet for 4 weeks: finely ground pellet (FP), coarsely ground meal (CM), coarsely ground pellet (CP) and coarsely ground extruded (CE) with dMEAN of 0.46, 0.88, 0.84 and 0.66 mm respectively. At the end of the feeding trial, the pigs were euthanized and the mandibular gland was collected after dissection. By immunohistochemistry, positive signals for CB1 were found in the cytoplasm of duct epithelial cells of pigs fed CP, FP and CE diets and in the serous cells of mixed acini in pigs fed the coarser CM diet. Positive signals for CB2 were detected in duct epithelial cells and in neurons of ganglia close to major secretory ducts of all pigs. The differential expression and localization of these receptors in response to variable chewing activity due to the type of diet suggest that endocannabinoids may influence the functional activity of the mandibular gland by modifying qualitative and/or quantitative aspects of salivary secretion.

show abstract

“…There have been several studies demonstrating that CB1 receptor is localized mainly in the ductal system, whereas CB2 receptor is in the acini in immunohistochemistry (Busch et al;Prestifilippo et al, 2006;Dall'Aglio et al, 2010). Considering the fact that the development and differentiation of the duct-acinus system of the salivary gland progress at early postnatal stages of mice (Zajicek et al, 1985;Denny et al, 1990), and also that the salivary glands are under controlled by their specific parasympathetic nervous system, it is meaningful to examine the localization of CB1 in the parasympathetic ganglion as well as in the glands during the course of postnatal development of mice by immunohistochemistry, which was the main purpose of the present study.…”

Section: Introductionmentioning

confidence: 99%

Localization of Cannabinoid Receptor 1 (CB1) in Submandibular and Sublingual Salivary Glands of Mice throughout Postnatal Development

2015

View full text Add to dashboard Cite

SUMMARY:In view of the fact that human marijuana users often show dry mouth symptom, the present study was attempted to examine the localization of CB1, which was originally identified in brain, in the submandibular and sublingual salivary glands of postnatal developing male mice by immunohistochemistry. In submandibular gland, CB1-immunoreactivity was positive in a majority of acinar cells in forms of granular appearance in their apical cytoplasm, while it was negative in the ducts at newborn stage. The immunoreactivity decreased in the acinar cells at P1W and no immunoreactivity was detected in the acinar cells at P3W and thereafter. The immunoreactivity was positive in ductal cells at P3W and it remained positive thereafter until P8W stage. The immunoreaction was distinct on the apical plasmalemma of the intercalated ductal cells, while it was distinct on the basal plasmalemma of the granular convoluted ductal cells. The enhanced immunostaining on the lateral plasmalemma of the granular ductal cells was discerned only on P6W. In sublingual gland, CB1-immunoreactivity was detected in the demilune acinar cells and ductal cells only on P4W. Furthermore, CB1-immunoreactivity was shown to occur in the salivary ganglionic neurons, suggesting the CB1-inhibitory action in the saliva secretion through the parasympathetic nervous transmission.

show abstract

Immunohistochemical localization of CB1 receptor in canine salivary glands

Cited by 25 publications

References 9 publications

Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system

Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system

The presence and distribution of cannabinoid type 1 and 2 receptors in the mandibular gland: The influence of different physical forms of diets on their expression in piglets

Localization of Cannabinoid Receptor 1 (CB1) in Submandibular and Sublingual Salivary Glands of Mice throughout Postnatal Development

Contact Info

Product

Resources

About