Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

Kagayama, Manabu; Akita, Hirotoshi; Sasano, Yasuyuki

doi:10.1007/bf00186744

Cited by 17 publications

(19 citation statements)

References 29 publications

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“…The temporospatial expression pattern of Amtn during tooth development needs further examination. Gja1 has been previously reported to be expressed in the cells of the stratum intermedium and the inner enamel epithelium (Pinero et al, 1994;Kagayama et al, 1995) where it is distributed exclusively at the sites of contact between odontoblasts, suggesting a function in cell to cell signaling (Fried et al, 1996). Mutations in Gja1 have been found to cause oculodentodigital dysplasia and Hallermann-Streiff syndrome, conditions that include dental anomalies (Paznekas et al, 2003;Pizzuti et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis

Pemberton

Oka

et al. 2007

Developmental Dynamics

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To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between postnatal day (P) 1 and P10 to identify genes differentially expressed when compared with 16 control tissues. Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by quantitative reverse transcription-polymerase chain reaction analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm). Developmental Dynamics 236: 2245-2257, 2007.

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Section: Discussionmentioning

confidence: 99%

Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis

Pemberton

Oka

et al. 2007

Developmental Dynamics

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“…Some investigations have found an association between CX43 and differentiation of odontoblasts (Pinero et al 1994;Kagayama et al 1995;Fried et al 14 1996;Murakami et al 2001). Murakami et al found that expression of CX43 showed an increase following odontoblast differentiation (Murakami et al 2001).…”

Section: Intercellular Communication Via Gjs Plays a Major Role In Mamentioning

confidence: 99%

“…Recently, investigations on expression and localization of CX43 in rat incisor odontoblasts and pulp cells have demonstrated that CX43 was highly expressed in young odontoblasts (Pinero et al 1994;Kagayama et al 1995;Fried et al 1996;Murakami et al 2001). Furthermore, CX43 has been shown to be a marker of viability in dental pulp tissue (Muramatsu et al 2004).…”

Section: +mentioning

confidence: 99%

Inhibition of connexin 43 expression and function in cultured rat dental pulp cells by antisense oligonucleotide

et al. 2007

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:Connexins are gap junction proteins forming hexameric structures in plasma membranes of adjacent cells, creating intercellular channels. Connexin 43 (CX43) is expressed in pulp tissue. However, its function in dental pulp tissue has yet to be fully investigated. In the present study, we employed antisense oligonucleotides (AS) against rat CX43 to investigate the role of CX43 in dental pulp cells. We treated cultured dental pulp cells with AS or sense (S) oligonucleotides. The number of cells in the AS-treated groups was about 1.3-fold that in the S-treated controls. Growth rates were significantly different between the AS-and S-treated groups at 48 h (p<0.01). An alkaline phosphate assay revealed that AS-treated pulp cells dramatically decreased at 48 h after AS incorporation, while S-treated pulp cells showed no marked changes.Western blot analysis showed that HSP25 was highly expressed in S-treated cells, but was only weakly expressed in AS-treated cells at 48 h. Furthermore, AS-treated cells showed high expression of CX45. On the other hand, S-treated cells showed high expression of CX32. These results suggest that CX43 is involved in cell growth, mineralization and differentiation to odontoblasts in rat pulp cells, and that CX43 plays the opposite role to that of CX45.3

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“…One freeze-fracture study showed that GJs increase in size with the growth of odontoblasts and that the largest GJs can be found in young odontoblasts, which suggests a correlation between the size of GJs and cell function in dentinogenesis (Iguchi et al 1984). More recent studies have demonstrated immunohistochemically that GJs are present in dental pulp tissue and that CX43 exists between odontoblasts (Pinero et al 1994;Kagayama et al 1995;Fried et al 1996). In contrast, Su et al (1997) found no CX43 expression in rat odontoblasts.…”

Section: Discussionmentioning

confidence: 92%

“…The presence of GJs between odontoblasts and nerve fibers has been suggested but has not been conclusively established (Gunji 1982;Koling and RaskAndersen 1984;Fried et al 1996). CX43 expression in odontoblasts has been demonstrated clearly in the developing molar tooth germ of rats (Pinero et al 1994;Kagayama et al 1995;Fried et al 1996), but the expression and localization of CX43 in rat incisal odontoblasts has not yet been examined.…”

Section: Introductionmentioning

confidence: 95%

Expression and localization of connexin 43 in rat incisor odontoblasts

Murakami

Muramatsu

Shimono

2001

Anatomy and Embryology

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We have examined the expression and localization of connexin 43 (CX43) in rat incisor odontoblasts using reverse transcriptase polymerase chain reaction, in situ hybridization and immunohistochemistry. The CX43 gene was expressed in odontoblasts, and levels of gene expression increased throughout the course of development. In contrast, CX43 was down-regulated at an incisal segment. In situ hybridization analysis showed no positive signal for CX43 RNA in the cytoplasm of differentiating dental papilla cells, but faint positive signals for CX43 RNA were observed in early pre-odontoblasts. Those signals were more intense in young and in old odontoblasts, but were less in short odontoblasts. CX43 could not be detected in differentiating dental papilla cells or in early pre-odontoblasts by immunohistochemical localization, but a positive reaction was found in the late pre-odontoblast stage where predentin had been produced. The positivity gradually increased during odontoblast maturation, and was highest in the layer of old odontoblasts. These results indicate that odontoblasts that secrete actively dentin matrix components are tightly in contact with each other by gap junctions as suggested by the intense CX43.

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Immunohistochemical localization of connexin 43 in the developing tooth germ of rat

Cited by 17 publications

References 29 publications

Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis

Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis

Inhibition of connexin 43 expression and function in cultured rat dental pulp cells by antisense oligonucleotide

Expression and localization of connexin 43 in rat incisor odontoblasts

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