Immunohistochemical localization of estrogen receptor-α in sex ducts and gonads of newborn piglets

Nielsen, Majken; Bøgh, Ingrid Brück; Schmidt, Mette; Greve, T.

doi:10.1007/s004180100269

Cited by 27 publications

(5 citation statements)

References 37 publications

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“…2001). Furthermore, the low level of oestrogens in the uterus of neonatal pigs might suggest that growth factors acting via ERα initiate the transcription of oestrogen‐dependent genes (Nielsen et al. 2001).…”

Section: Discussionmentioning

confidence: 99%

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

Knapczyk-Stwora

Durlej

Duda

et al. 2011

Reprod Domestic Animals

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The uterus is a well-known target of endocrine, paracrine and autocrine acting molecules among which steroid hormones are of special importance. The objective of our work was to localize oestrogen receptors (ERα and ERβ) mRNA and protein in the pig uterus throughout pregnancy (10, 18, 32, 50, 71, 90 days post coitum) using RT-PCR, Western-blot and immunohistochemistry. The present study is the first one to demonstrate the presence of ERs protein in the porcine uterus not only at the beginning but also at mid- and late pregnancy. In the pregnant swine, ERα was immunolocalized in the luminal epithelium (LE) and glandular epithelium (GE) and the myometrium of the uterus with differences in the intensity of staining at different stages of pregnancy studied. The LE and GE of pregnant swine stained for ERβ regardless of the day of pregnancy examined, whereas only a few cells within the myometrium showed a weak immunoreactivity. Western blot analysis confirmed the presence of ERα and ERβ proteins on all investigated days of gestation. The expression of ERα and ERβ mRNA was detected by RT-PCR in all examined samples corresponding to each of the consecutive stages of pregnancy. The obtained results show that ERα is more abundant in comparison to ERβ within the porcine pregnant uterus. The presence of ERα and ERβ in all compartments of the pig uterus during pregnancy may indicate direct action of oestrogens on proliferation and differentiation of these cells.

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Section: Discussionmentioning

confidence: 99%

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

Knapczyk-Stwora

Durlej

Duda

et al. 2011

Reprod Domestic Animals

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“…CD151 has previously been linked with human gamete fusion 22 , associates specifically with α3, α6 and α7 integrins 1,3,23 and links α3β1 and α6β1 integrins to other tetraspanins, including CD9 [24][25][26][27] . Based on that knowledge we propose that CD151 could be involved as a part of known multimolecular complexes remodelling sperm membrane through integrins 28,29 . Remodelling of the acrosomal region of the sperm head during acrosome reaction (AR) has been reported 37 , the other bands may easily represent CD151 isoforms with different glycosylation, however, the un-glycosylated form was found only in human spermatozoa (Fig.…”

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confidence: 93%

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Jankovičová

Frolikova

Palenikova

et al. 2020

Sci Rep

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The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence of CD151 in sperm, and present its expression and distribution during spermatogenesis and sperm transition during the acrosome reaction. We traced CD151 gene and protein expression in testicular cell subpopulations, with strong enrichment in spermatogonia and spermatids. The testicular and epididymal localization pattern is designated to the sperm head primary fusion site called the equatorial segment and when compared to the acrosome vesicle status, CD151 was located into the inner acrosomal membrane overlying the nucleus. Moreover, we show CD151 interaction with α6 integrin subunit, which forms a dimer with β4 as a part of cisprotein interactions within sperm prior to gamete fusion. We used mammalian species with distinct sperm morphology and sperm maturation such as mouse and bull and compared the results with human. In conclusion, the delivered findings characterise CD151 as a novel sperm tetraspanin network member and provide knowledge on its physiology in male germ cells.CD151 belongs to the tetraspanin superfamily, these proteins participate in many biological processes such as growth control, intracellular signalling, cell adhesion, migration, motility 1-4 and they also take place in pathogenesis of some human diseases 5,6 . Tetraspanins create a scaffolding multiprotein network in the membrane that anchors other proteins into specific domains with precise functions and moreover, they are indispensable players in the mammalian fertilization process. Tetraspanin CD9 expressed on mouse eggs is considered as an essential molecule for successful gamete fusion 7-9 as well as tetraspanin CD81 which is also involved in fertilization 10,11 . Our previous studies concerning species-specific traits of CD9 and CD81 in mouse, bull and porcine gametes during their functional maturation and fertilization, suggested the active participation of tetraspanins in these events [11][12][13][14] . With the ability of tetraspanins being part of signalling cascades involving cytoskeleton 5,15,16 they also play a crucial role in the organization of functional membrane protein cluster domains called a tetraspanin web, which were also described in oocyte and recently in sperm. The integral part of the tetraspanin web are integrins and the presence of α3β1, α6β1 and α6β4 integrins was shown in sperm [17][18][19][20] . Importantly, in somatic cells, tetraspanin CD151 was found to play a key role in regulating the adhesion strengthening of integrin α6β1 21 . CD151 has previously been linked with human gamete fusion 22 , associates specifically with α3, α6 and α7 integrins 1,3,23 and links α3β1 and α6β1 integrins to other tetraspanins, including CD9 [24][25][26][27] . Based on that knowledge we p...

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“…Two subtypes of ER exist, ERα and ERβ; these proteins have been found in the uterus of various animal species (Greene et al, ; Kuiper, Enmark, Pelto‐Huikko, Nilsson, & Gustafsson, ; Shughrue, Lane, Scrimo, & Merchenthaler, ; Silva et al, ; Wang, Masironi, Eriksson, & Sahlin, ). Studies have shown that ERα antagonists can inhibit the development of pig uterine glands and lead to delayed development of endometrium (Nielsen, Bogh, Schmidt, & Greve, ). In ERα knockout mice with uterine hypoplasia, the number of uterine glands was significantly reduced, and the rate of pregnancy in ERβ knockout mice was significantly diminished (Couse & Korach, ), indicating that ER is important for the function of the uterus.…”

Section: Introductionmentioning

confidence: 99%

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Duan

Xiao

et al. 2019

Reprod Domestic Animals

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Oestrogen, androgen and progesterone are involved in the regulation of uterine physiological functions, with the participation of the following proteins: oestrogen receptor (ER), androgen receptor (AR) and progesterone nuclear receptor (PGR). In this study, we used immunohistochemistry to detect the localization of ERα, ERβ, AR and PGR in sheep uterus. Additionally, we used real‐time polymerase chain reaction (RT‐qPCR) and Western blot technique to analyse their expression profiles at different stages of sheep oestrous cycle in the endometrium and myometrium. Immunohistochemical analysis showed that ERα, ERβ, AR and PGR were present in sheep uterus in oestrus, mainly in the uterine luminal epithelium, stroma, gland and myometrium. Real‐time polymerase chain reaction results showed that in the endometrium, ERα expression level was highest in oestrus. ERβ and PGR, instead, were highly expressed in pro‐oestrus. In the myometrium, ERα was highly expressed in both oestrus and pro‐oestrus, and ERβ was highly expressed in oestrus and dioestrus. Progesterone nuclear receptor expression was highest in oestrus, followed by metoestrus. In the endometrium, both receptors ERα and ERβ were abundant in pro‐oestrus, while the maximum AR protein content was found in oestrus. At this stage of the oestrous cycle, PGR protein concentration in the myometrium was significantly lower than those observed in other stages. These results suggest that these receptors are important for sheep reproductive function, as their expression at mRNA and protein levels exhibits particular time‐ and tissue‐specific profiles along the oestrous cycle.

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Immunohistochemical localization of estrogen receptor-α in sex ducts and gonads of newborn piglets

Cited by 27 publications

References 37 publications

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

Expression of Oestrogen Receptor α and Oestrogen Receptor β in the Uterus of the Pregnant Swine

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

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