Immunohistochemical localization of irisin in mole rats(Spalax leucodon)

Abstract

Irisin was first identified in skeletal muscle cells. It is an exercise protein that is secreted into the circulation; it causes conversion of white adipose tissue to brown adipose tissue. We investigated irisin immunoreactivity in mole rat (Spalax leucodon) tissues. We examined cerebellum, pituitary, heart, liver, pancreas, spleen, uterus, kidney and striated muscle in female adult mole rats. Tissues were processed, embedded in paraffin, sectioned at 5 μm and stained immunohistochemically for irisin. Irisin immunostaining was detected in the cytoplasm of stained cells; the cytoplasm of Purkinje cells was unstained. We found that irisin may be synthesized in many tissues. The function of locally synthesized irisin currently is unknown.

From Exercise to Cognitive Performance: Role of Irisin

“…Irisin is cleaved from fibronectin type III domain containing 5 (FNDC5), a transmembrane precursor protein expressed in muscle under the control of PGC-1α. FNDC5 is also known to be profoundly expressed in many regions of thebrain, including cerebellar Purkinje cells, the hypothalamus, and the hippocampus, a region of the brain involved in memory and spatial awareness [7][8][9][10][11][12].…”

Revisiting skeletal myopathy and exercise training in heart failure: Emerging role of myokines

“…These clinical findings are supported by experimental data showing abnormally reduced FNDC5 and PGC-1α expression in skeletal muscle from rats with HFrEF secondary to ischemic cardiomyopathy [42]. Irisin can also be produced by the heart [43]. Compared with wild-type animals, Fndc5 gene deletion resulted in exacerbation of LV hypertrophy, dysfunction and fibrosis, whereas Fndc5 gene overexpression resulted in attenuation of these phenotypes [44].…”

Effect of Irisin on LIF and integrin αvβ3 in rats of implantation failure

“…Existing evidence of the role of biological factors, such as leptin, insulin and adiponectin, whose main function is to regulate energy metabolism, in reproduction is conclusive [7,8]. Moreover, studies have shown that the expression of irisin has been identified in endometrium and ovary [9,10]. Based on these studies, we speculate that, irisin, a new molecular marker and target in metabolic disorder [11], may have potential effect on reproductive capacity.…”

“…Studies demonstrated that the expression of irisin has been found in endometrium and endometrial irisin level in PCOS patients decreased [9,10], which indicated a potential direct role of irisin on endometrium. Embryo implantation is the premise of successful pregnancy, it is crucial to take timely modifications for endometrium to become receptive to the developing embryo for success of implantation [12].…”