Immunohistochemical localization of lysozyme, lactoferrin, al-antichymotrypsin, and α1-antichymotrypsin, and α1-antitrypsin in salivary gland of human fetuses

Lee, Suk-Keun; Lim, Chae Young; G., Je; Yamada, Kazuto; Kunikata, Mayuko; Hashimura, Kouji; Mori, Masahiko

doi:10.1016/s0065-1281(11)80357-0

Cited by 7 publications

(9 citation statements)

References 27 publications

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“…3,20,24 Moreover, only a few studies on salivary glands in infants have been reported. Lee et al 25 investigated the localization of lysozyme, lactoferrin, α1-antichymotrypsin, and α1-antitrypsin in major salivary glands of human fetuses. Host defense peptides of the entire lip vermilion mucosa were studied by RT-PCR in infants.…”

Section: Research-article2016mentioning

confidence: 99%

Labial Salivary Glands in Infants

Stoeckelhuber

Loeffelbein

Olzowy

et al. 2016

J Histochem Cytochem.

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SummaryHuman labial glands secrete mucous and serous substances for maintaining oral health. The normal microbial flora of the oral cavity is regulated by the acquired and innate immune systems. The localization and distribution of proteins of the innate immune system were investigated in serous acinar cells and the ductal system by the method of immunohistochemistry. Numerous antimicrobial proteins could be detected in the labial glands: β-defensin-1, -2, -3; lysozyme; lactoferrin; and cathelicidin. Cytoskeletal components such as actin, myosin II, cytokeratins 7 and 19, α-and β-tubulin were predominantly observed in apical cell regions and may be involved in secretory activities. (J Histochem Cytochem 64:502-510, 2016)

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Section: Research-article2016mentioning

confidence: 99%

Labial Salivary Glands in Infants

Stoeckelhuber

Loeffelbein

Olzowy

et al. 2016

J Histochem Cytochem.

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“…Synthesis and subcellular localization of LF in salivary gland cells have been detected. This indicates the secretory mechanism of LF by acinar cells (185,186). LF is expressed in both normal salivary gland cells and tumors cells (187)(188)(189)(190)(191)(192)(193).…”

Section: Lysozyme Lactoferrin 1 -Antichymotrypsin and 1 -Antitrypsinmentioning

confidence: 92%

“…In these studies, the coexpression of LF with LY has been demonstrated in salivary gland tumors. LF reactivity in human fetal salivary glands is demonstrated in serous acinar cells (25 w to 40 w fetus) and in intercalated, striated and excretory ducts (25 w to 29 or 30 w) (186).…”

Section: Lysozyme Lactoferrin 1 -Antichymotrypsin and 1 -Antitrypsinmentioning

confidence: 98%

“…Localization of LY in salivary glands has been investigated with an immunohistochemical method, which demonstrated that ductal cells are strongly positive and serous acinar cells are occasionally reactive. Human fetal salivary glands expressed LY in acinar cells (usually serous type), and in intercalated duct cells in specimens from 21, 23 to 40 weeks (185,186). Salivary pleomorphic adenoma also shows positive reaction for LY and LF.…”

Section: Lysozyme Lactoferrin 1 -Antichymotrypsin and 1 -Antitrypsinmentioning

confidence: 99%

“…Human salivary glands are reactive to 1 -AT in ductal segments but unreactive to -ACT in glandular components (202). In human fetal glands, -ACT is present in serous cells (23-34 w) and in ducts (15-20 w), while -AT is expressed in both serous and mucous cells (20-40 w) and ducts (15-40 w) at strong level (186). Salivary pleomorphic adenoma and other tumors are found to show varying reactivities to -ACT and -AT (202).…”

Section: Lysozyme Lactoferrin 1 -Antichymotrypsin and 1 -Antitrypsinmentioning

confidence: 99%

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Antimicrobial Peptides in Saliva and Salivary Glands: Their Roles in the Oral Defense System

et al. 2006

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Elafin expression in human fetal and adult submandibular glands

Lee

Hirose

et al. 2002

Histochem Cell Biol

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Elafin, a bifunctional protein, has the NH(2)-terminal domain functions as a transglutaminase substrate for crosslinking to lysine-containing proteins and the COOH-terminal whey acidic protein domain as a potent anti-elastase. Human fetal submandibular glands (n=100) and adult submandibular glands (n=10) were used to elucidate the expression pattern of elafin in the developmental processes of human submandibular gland by immunohistochemistry, in situ hybridization, and western blot analysis. Elafin mRNA was expressed both in the gland epithelium and intralobular mesenchymal tissue of fetal submandibular gland in an early developmental stage (10-18 weeks) and an early intermediate developmental stage (EIDS; 19-24 weeks). The elafin antigen was also found in the intralobular mesenchyme of submandibular gland in the same stages. Thereafter, elafin was transitionally expressed in the ducts and acini of submandibular glands. In the late intermediate developmental stage (LIDS; 25-32 weeks) and the late developmental stage (LDS; 33-40 weeks), elafin became markedly positive in the excretory and striated ducts but weakly positive in the intralobular mesenchymal tissue. The elafin was heavily present in the excretory and striated ducts of adult submandibular gland, while it was sparse in the intralobular mesenchymal tissues. Western blot analysis showed the protein extracts from submandibular glands of EIDS, LIDS, and LDS, adult submandibular gland, fetal tissues (8 weeks), and adult parotid saliva migrated into multiple bands, about 25, 50, 65, and 140 kDa, which were higher than the putative size of elafin protein, 12 kDa. These data suggest that elafin, anti-elastase, is an essential component highly utilized during the morphogenetic processes of fetal salivary gland development and continuously plays a role for the protection of the tubuloalveolar structures of adult salivary gland.

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Immunohistochemical localization of lysozyme, lactoferrin, al-antichymotrypsin, and α1-antichymotrypsin, and α1-antitrypsin in salivary gland of human fetuses

Cited by 7 publications

References 27 publications

Labial Salivary Glands in Infants

Labial Salivary Glands in Infants

Antimicrobial Peptides in Saliva and Salivary Glands: Their Roles in the Oral Defense System

Elafin expression in human fetal and adult submandibular glands

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