Immunohistochemical localization of orexin A and orexin type 2 receptor-positive cells in the placenta of dogs

Dall’Aglio, Cecilia; Polisca, Angela; Troisi, Alessandro; Zelli, Riccardo; Ceccarelli, Piero

doi:10.1016/j.acthis.2014.03.004

Cited by 13 publications

(7 citation statements)

References 17 publications

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“…Orexin A could also be released from peripheral tissues such as the gastrointestinal tract, the endocrine pancreas β ‐cells, and kidney . Importantly, we found that orexin A is expressed in HUVECs, which is consistent with a previous study showing that the endothelial cells of the capillaries near the uterine glands express orexin A . It is possible that orexin A produced in endothelial cells on the vascular wall enters into the bloodstream.…”

Section: Discussionsupporting

confidence: 92%

Orexin A Suppresses Oxidized LDL Induced Endothelial Cell Inflammation via MAPK p38 and NF‐κB Signaling Pathway

Zhang

Liang

et al. 2018

IUBMB Life

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Orexin A is a multifaceted peptide produced in hypothalamus. We examined the effect of orexin A on vascular endothelial cells. Our study showed that orexin A had a profound inhibitory effect against endothelial inflammation by oxidized low-density lipoprotein (ox-LDL) in endothelial cells. Orexin A partially suppressed ox-LDL-induced monocytes THP-1 cells attachment to endothelial cells by limiting expression of vascular molecules including VCAM-1, ICAM-1, and E-selectin. Mechanistically, orexin A ameliorated endothelial dysfunction by reducing MAP kinase p38 and NF-κB activation via its receptor-OX1R. Orexin A suppressed phosphorylation of MAP kinase p38 and the NF-κB cascade kinases IKKα and IκBα, and prevented the shuttle of p65 protein into nuclear. Additionally, we reported that OX1R was expressed in HUVECs. Silence of OX1R completely abolished the inhibitory function of orexin in attachment of THP-1 cells. Collectively, our data suggest that orexin A ameliorated endothelial dysfunction under inflammatory stimuli. © 2018 IUBMB Life, 70(10):961-968, 2018.

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Section: Discussionsupporting

confidence: 92%

Orexin A Suppresses Oxidized LDL Induced Endothelial Cell Inflammation via MAPK p38 and NF‐κB Signaling Pathway

Zhang

Liang

et al. 2018

IUBMB Life

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“…Hcrts/orexins have also been identified in the kidney, albeit at a much lower concentration compared with those measured in the brain . Hcrts/orexins is also discernible in tear‐producing lacrimal, and adrenal glands and in placenta . Real‐time polymerase chain reaction studies showed that the major salivary glands (parotid, submandibular, and sublingual) of mammals have also been shown to contain Hcrt/orexin and their receptors .…”

Section: Tissue Expression Of Hypocretin/orexin Systemmentioning

confidence: 99%

“…41 Hcrts/orexins is also discernible in tear-producing lacrimal, 42 and adrenal 26 glands and in placenta. 28 Real-time polymerase chain reaction studies showed that the major salivary glands (parotid, submandibular, and sublingual) of mammals have also been shown to contain Hcrt/orexin and their receptors. 43 Dall'Aglio et al 43 used immunohistochemistry to identify orexin and their receptors in ganglion cells and ducts of the submandibular but not in parotid and sublingual glands (Table 1).…”

Section: Tissue Expression Of Hypocretin/ Orexin Systemmentioning

confidence: 99%

Hypocretin/orexin modulates body weight and the metabolism of glucose and insulin

Adeghate

Lotfy

D’Souza

et al. 2020

Diabetes Metabolism Res

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Summary The hypocretin/orexin (Hcrt/orexin) unit affects the functions of the nervous, cardiovascular, gastrointestinal, and reproductive systems. Hcrt/orexin ligands and receptors have been localized to different parts of the central and peripheral nervous systems, cerebrospinal fluid and blood, exocrine (pancreas, salivary, lacrimal) as well as endocrine (pancreatic islets, pituitary, adrenal) glands. Several factors including stress, glucagon‐like peptide‐1 agonists, glutamate, nicotine, glucose, and hypoglycaemia stimulate the expression of Hcrt/orexin system, but it is inhibited by ageing, bone morphogenetic protein, hypoxia/hypercapnia, melanocortin receptor accessory protein 2, and glucagon. Literature reports show that Hcrt/orexin can significantly increase insulin secretion from normal and diabetic rat pancreata. Hcrt/orexin decreases blood glucose concentration and reduces insulin resistance partly via increased tissue expression of glucose transporter type 4. It reduces obesity by increasing browning of fat cells and energy expenditure. Taken together, Hcrt/orexin modulates obesity and the metabolism of glucose and insulin. The Hcrt/orexin system may thus be a target in the development of new therapies for the treatment of diabetes mellitus.

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“…For the molecular biological studies, the samples were rinsed with RNase-free water and then frozen at -80 C for later evaluation of gene and protein expression. For the immunohistochemical analysis, other tissue samples of testes were xed by immersion in 4% (w/v) formaldehyde in PBS (pH 7.4) for 24 h at room temperature and subsequently processed following routine tissue preparation procedures (Dall'Aglio et al, 2014).…”

Section: Tissue Collection and Processingmentioning

confidence: 99%

Presence and localization of apelin and its cognate receptor in the testis of the dog using immunohistochemical and molecular biology technique.

Troisi

Dall’Aglio

Maranesi

et al. 2022

Preprint

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Apelin, a member of the adipokine family, is a new endogenous peptide and it’s involved, by interacting with a specific receptor, in the control of human and laboratory animals’male reproduction. As far as we know, no data is available about the presence of the apelinergic system in dog testicles. Therefore the aim of this study was to show, for the first time, its presence and distribution in the canine testis by immunoistochemistry and molecular biology studies. For this purpose, five fertile and healthy male dogs were used and subjected to elective orchiectomy. The immunohistochemical reaction evidenced the presence of apelin and its receptor in the canine testis. In particular, apelin appeared localized in spermatids and spermatozoa with a positive signal in the “acrosomal bodies”. Regarding to the apelin receptor, a positive immunoreaction was evidenced in the cytoplasm of the cells localized in the basal portion of the seminiferous tubules, likely Sertoli’s cells, and in the cytoplasm of Leydig cells. The RT-PCR analysis showed the presence of transcripts for apelin and apelin receptor in all the samples examined. In conclusion our result allows us to hypothesize that the role of the paracrine and endocrine apelinergic system reported in laboratory animals can also be found in the dog.

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Immunohistochemical localization of orexin A and orexin type 2 receptor-positive cells in the placenta of dogs

Cited by 13 publications

References 17 publications

Orexin A Suppresses Oxidized LDL Induced Endothelial Cell Inflammation via MAPK p38 and NF‐κB Signaling Pathway

Orexin A Suppresses Oxidized LDL Induced Endothelial Cell Inflammation via MAPK p38 and NF‐κB Signaling Pathway

Hypocretin/orexin modulates body weight and the metabolism of glucose and insulin

Presence and localization of apelin and its cognate receptor in the testis of the dog using immunohistochemical and molecular biology technique.

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