Immunohistochemical localization of orexin/hypocretin-like immunoreactive peptides and melanin-concentrating hormone in the brain and pituitary of medaka

Amiya, Noriko; Amano, Masafumi; Oka, Yoshitaka; Iigo, Masayuki; Takahashi, Akiyoshi; Yamamori, Kunio

doi:10.1016/j.neulet.2007.07.043

Cited by 49 publications

(38 citation statements)

References 22 publications

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“…This result is in line with similar observations from several studies in teleosts, amphibians, reptiles, birds, and mammals [Shibahara et al, 1999;Galas et al, 2001;Phillips-Singh et al, 2003;Kaslin et al, 2004;Huesa et al, 2005;Singletary et al, 2005Singletary et al, , 2006Nixon and Smale, 2007;. In contrast, orexins in specific cells of the adenohypophysis have been reported in human [Blanco et al, 2003], orexin-A in two species of anurans [Yamamoto et al, 2004;Suzuki et al, 2007], and orexin-A and orexin-B in the pituitary of two species of teleost fishes [Amiya et al, 2007;Suzuki et al, 2007Suzuki et al, , 2009.…”

Section: Distribution Of Orexin-ir Fibers In the Brain Of Vertebratessupporting

confidence: 90%

“…The presence of these cells in the goldfish (Carassius auratus) brain is more restricted and they are located in the anterolateral hypothalamus within the nucleus lateralis tuberis and, more caudally, bordering the third ventricle in the nucleus posterioris periventricularis [Huesa et al, 2005]. The latter nucleus contains the main group of orexin-ir cells in the medaka fish Oryzias latipes [Amiya et al, 2007].…”

Section: Orexin-ir Neurons In the Brain Of Vertebratesmentioning

confidence: 99%

“…Interestingly, the primary amino acid sequence of orexins is highly conserved across vertebrates [Sakurai et al, 1998;Shibahara et al, 1999;Alvarez and Sutcliffe, 2002;Ohkubo et al, 2002;Panula, 2009]. Thus, the distribution of orexin-like immunoreactive cells and fibers in the brain of different vertebrates has been analyzed by means of immunohistochemical techniques using antibodies against mammalian orexins [Shibahara et al, 1999;Galas et al, 2001;Ohkubo et al, 2002;PhillipsSingh et al, 2003;Kaslin et al, 2004;Huesa et al, 2005;Singletary et al, 2005Singletary et al, , 2006Amiya et al, 2007;. In general, the localization of orexin-ir cell bodies in particular forebrain nuclei and the widespread distribution of fibers were corroborated for all vertebrate groups studied, but when direct cross-species comparisons were made, using the same immunohistochemical approach, important variations were found in the precise distribution of orexin-ir cells and fibers [Singletary et al, 2006;Nixon and Smale, 2007;.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes Protopterus dolloi and Neoceratodus forsteri

et al. 2009

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Lungfishes are currently considered the closest living relatives of tetrapods and represent an interesting group for the study of evolutionary traits in the transition from fishes to tetrapods. The brains of lungfishes have received little attention in comparative studies probably due to the difficulty of obtaining these unique animals. In the present study the distribution of orexin (hypocretin)-like immunoreactivity was studied in the brain of the African lungfish Protopterus dolloi and the Australian lungfish Neoceratodus forsteri by using antibodies directed against the mammalian orexin-A and orexin-B peptides. Simultaneous detection of orexins and tyrosine hydroxylase or serotonin was used to assess the precise location of the orexins in the brain and to evaluate the possible influence of the orexin system on the monoaminergic cell groups. Although some differences were noted, a common pattern for the distribution of orexins in the two lungfishes studied was observed. In both species, most immunoreactive neurons were observed in the suprachiasmatic nucleus and dorsal hypothalamus. Only in Neoceratodus, however, were important cell populations found in the preoptic area and infundibular hypothalamus, whereas small numbers of faintly reactive neurons were present in the lateral septum and ventral striatum. Fiber labeling was widely distributed in all main brain subdivisions, but was more abundant in regions such as the septum, preoptic area, suprachiasmatic nucleus, lateral hypothalamic area, thalamus, pretectum and tegmentum. Less conspicuous was the innervation of the pallial regions, habenula, optic tectum, rhombencephalic reticular formation and spinal cord. Orexinergic innervation was found in close contact with dopaminergic, noradrenergic and serotoninergic cell groups, homologous to the substantia nigra in the midbrain tegmentum, the locus coeruleus, the nucleus of the solitary tract and the raphe nuclei. Although unique features have been found for lungfishes, the location of orexin immunoreactive elements is largely consistent with that recently reported following a similar approach in amphibians and amniotes, suggesting that the general organization of this peptidergic system occurred in the common ancestor of lungfishes and tetrapods.

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Section: Distribution Of Orexin-ir Fibers In the Brain Of Vertebratessupporting

confidence: 90%

Section: Orexin-ir Neurons In the Brain Of Vertebratesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes Protopterus dolloi and Neoceratodus forsteri

et al. 2009

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“…Neuronal cell bodies with MCH-like immunoreactivity are distributed mainly in the thalamus and hypothalamus including the paraventricular organ (PVO) and the nucleus lateralis tuberis (NLT), and their fibres project to the neurohypophysis of teleosts (Vallarino et al 1989, 1998, Baker et al 1995, Amano et al 2003, Pandolfi et al 2003, Amiya et al 2007, Matsuda et al 2009a. These reports suggest that nerve fibres containing MCH-like immunoreactivity arise from the thalamic and hypothalamic areas, and terminate mainly in the neurohypophysis, and that MCH acts as a peripheral hormone affecting body colour in fish.…”

Section: Introductionmentioning

confidence: 99%

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Tanaka¹,

Azuma²,

Nejigaki³

et al. 2009

Journal of Endocrinology

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Melanin-concentrating hormone (MCH)-containing neurons directly innervate the adenohypophysis in the teleost pituitary. We examined immunohistochemically the relationship between MCH-containing nerve fibres or endings and somatolactin (SL)-producing cells in the goldfish pituitary. Nerve fibres or endings with MCH-like immunoreactivity were identified in the neurohypophysis in close proximity to the adenohypophysial cells showing SL-like immunoreactivity. We also examined the effect of MCH on SL release from cultured goldfish pituitary cells and SL synthesis using a cell immunoblot and a real-time PCR method. Treatment of individually dispersed pituitary cells with MCH 10 K7 M for 3 h decreased the area of SL-like immunoreactivity on immunoblots, and MCH-induced reductions in SL release were blocked by treatment with the mammalian MCH receptor (MCHR) antagonist, compound-30, at a concentration of 10 K5 M.Treatment with 10 K7 M MCH for 3 h did not affect sl-a and -b (smtla and -b as given in the Zfin Database) mRNA expression levels. These led us to explore the signal transduction mechanism leading to the inhibition of SL release, for which we examined whether MCH-induced reductions in SL release are mediated by the G i or G q proteincoupled signalling pathway. The MCH-induced reductions in SL release were abolished by treatment with the G i/o protein inhibitors, NF023 (10 K5 M) or pertussis toxin (260 ng/ml), but not by the phospholipase C inhibitor, U-73122 (3!10 K6 M). These results indicate that MCH can potentially function as a hypothalamic factor suppressing SL release via the MCHR, and subsequently through the G i protein to inhibit the adenylate cyclase/cAMP/protein kinase A-signalling pathway in goldfish pituitary cells.

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“…In mammals, orexins are produced by a small population of neurons located in the lateral hypothalamus that profusely innervates all major subdivisions of the central nervous system Cutler et al, 1999;Nambu et al, 1999;McGranaghan and Piggins, 2001;Mintz et al, 2001;Novak and Albers, 2002;Sakurai, 2005;Nixon and Smale, 2007]. The highly phylogenetic conserved molecular structure of these neuropeptides [Shibahara et al, 1999;Wong et al, 2011] has allowed the immunohistochemical localization of orexinlike immunoreactive cells and fibers in the brain of nonmammalian vertebrates like birds [Ohkubo et al, 2002;Singletary et al, 2006;Miranda et al, 2013], reptiles [Domínguez et al, 2010b], amphibians [Shibahara et al, 1999;Galas et al, 2001;López et al, 2009a], and distinct fish groups like lungfishes [López et al, 2009b], teleosts [Kaslin et al, 2004;Huesa et al, 2005;Amiya et al, 2007;Panula, 2010;Matsuda et al, 2012;Pérez Sirkin et al, 2013], and cladistians [López et al, 2014]. All of these studies point to a conserved organization of the orexinergic system among vertebrates, with a general presence of orexin neurons in the hypothalamus (although the particular localization within this region can vary among vertebrate groups or even among species within the same group) and a pattern of extensive innervation on the main brain areas.…”

mentioning

confidence: 99%

Spatiotemporal Development of the Orexinergic (Hypocretinergic) System in the Central Nervous System of Xenopus laevis

López¹,

Morales²,

González³

2016

Brain Behav Evol

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The present immunohistochemical study represents a detailed spatiotemporal analysis of the localization of orexin-immunoreactive (OX-ir) cells and fibers throughout development in the brain of the anuran amphibian Xenopus laevis, a model frequently used in developmental studies. Anurans undergo remarkable physiological changes during the early life stages, and very little is known about the ontogeny and the localization of the centers that control functions such as appetite and feed ingestion in the developing brain. We examined the onset of the orexinergic system, demonstrated to be involved in appetite regulation, using antibodies against mammalian orexin-A and orexin-B peptides. Simultaneous detection of orexins with other territorial markers was used to assess the precise location of the orexinergic cells in the hypothalamus, analyzed within a segmental paradigm. Double staining of orexins and tyrosine hydroxylase served to evaluate possible interactions with the catecholaminergic systems. At early embryonic stages, the first OX-ir cells were detected in the hypothalamus and, soon after, long descending projections were observed through the brainstem to the spinal cord. As brain development proceeded, the double-staining techniques demonstrated that this OX-ir cell group was located in the suprachiasmatic nucleus within the alar hypothalamus. Throughout larval development, the number of OX-ir cells increased notably and a widespread fiber network that innervated the main areas of the forebrain and brainstem was progressively formed, including innervation in the posterior tubercle and mesencephalon, the locus coeruleus, and the nucleus of the solitary tract where catecholaminergic cells are present. In addition, orexinergic cells were detected in the preoptic area and the tuberal hypothalamus only at late prometamorphic stages. The final distribution pattern, largely similar to that of the adult, was achieved through metamorphic climax. The early expression of orexins in Xenopus suggests important roles in brain development in the embryonic period before feeding, and the progression of the temporal and spatial complexity of the orexinergic system might be correlated to the maturation of appetite control regulation, among other functions.

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Immunohistochemical localization of orexin/hypocretin-like immunoreactive peptides and melanin-concentrating hormone in the brain and pituitary of medaka

Cited by 49 publications

References 22 publications

Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes <i>Protopterus dolloi</i> and <i>Neoceratodus forsteri</i>

Distribution of Orexin/Hypocretin Immunoreactivity in the Brain of the Lungfishes <i>Protopterus dolloi</i> and <i>Neoceratodus forsteri</i>

Melanin-concentrating hormone reduces somatolactin release from cultured goldfish pituitary cells

Spatiotemporal Development of the Orexinergic (Hypocretinergic) System in the Central Nervous System of <b><i>Xenopus laevis</i></b>

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