Immunohistochemical localization of substance P, vasoactive intestinal polypeptide and gastrin-releasing peptide in vas deferens and seminal vesicle, and the effect of these and eight other neuropeptides on resting tension and neurally evoked contractile activity

Stjernquist, Martin; Håkanson, R.; Leander, S.; Owman, Christer; Sundler, F.; Uddman, R.

doi:10.1016/0167-0115(83)90282-3

Cited by 73 publications

(53 citation statements)

References 22 publications

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“…Although ejaculation has been thought of as a single event, it involves three distinct components: emission, ejection and orgasm. 1 Emission is a Ring preparation method in isolated rat SV experiment HW Lee et al sympathetically mediated neural function involving the release of ductal ejaculatory closure resistance and the coordinated contraction of the SVs and ductus deferens smooth muscles. The sympathetic signals cause the release of NE from the postganglionic neurons of the seminal tract to activate smooth muscle 1-adrenergic receptors.…”

Section: Discussionmentioning

confidence: 99%

“…1 The human SVs are a lateral outpouching of the vas deferens with a capacity of 3-4 ml; these tubuloalveolar glands are the major sites for the production and storage of seminal fluid and consist of a mucosal layer lining the lumen, and a thick outer layer of circular and longitudinal smooth muscle. 2 The rhythmic contractions of the smooth muscle of the SVs are vital to the facilitation of seminal fluid discharge.…”

Section: Introductionmentioning

confidence: 99%

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A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation

et al. 2008

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In this study, we evaluated the efficacy of a novel method of seminal vesicle (SV) preparation-ring preparation method-in isolated SV experiments in the rat. Rat SVs were prepared as strips and rings and applied to organ baths. The relaxation responses by sodium nitroprusside or doxazosin and contractile responses by electrical field stimulation (EFS) were recorded in both groups. We compared the proportion of tissues that showed consistent responses to the stimuli-drug or EFSin both groups. And magnitudes of the contractile or relaxation responses were also evaluated in the two groups. In strip preparations (n ¼ 20), six tissues (30%) showed stable responses to drugs and were regarded to be useful. However, in ring preparations (n ¼ 20), 13 tissues (65%) showed stable responses (Po0.001). Similarly, consistent contractile responses to the EFS were recorded in eight strips (40%) and 13 rings (65%) (n ¼ 20 in each group; Po0.05). The magnitudes of the relaxations by drugs were not different between the two methods (P ¼ 0.42) and the contractions by EFS were higher in ring preparations (Po0.05). The ring preparation seems to be better for responses to drugs and EFS compared with the strip preparations and, therefore, might be an alternative technique for in vitro SV experiments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation

et al. 2008

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“…VIP has been demonstrated in nerve fibres supplying the prostate and seminal vesicles in the guinea-pig, rat, cat, rabbit, mouse and humans. VIP-immunoreactive nerve fibres have been mainly associated with the basis of the glandular epithelium and, in smaller proportion, with the smooth muscle coat and blood vessels (Alm et al, 1980;Vaalasti et al, 1980Vaalasti et al, , 1986Larsen et al, 1981;Gu et al, 1983;Stjernquist et al, 1983;Polak and Bloom, 1984;Lamano Carvalho et al, 1986;Higgins and Gosling, 1989;Lange and Unger, 1990;Properzi et al, 1992;Jen et al 1995;Kepper and Keast, 1995;Tainio, 1995). Very little is known about the physiological role of VIP in AGG.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Klimczuk¹,

Kaleczyc²,

Franke-Radowiecka³

et al. 2005

Vet. Med. - Czech

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Our previous immunohistochemical investigations revealed three major populations of nerve fibres supplying the porcine accessory genital glands (AGG) including noradrenergic, non-noradrenergic putative cholinergic and sensory nerve terminals (Kaleczyc et al., 1997). However, it is still unclear whether the non-noradrenergic nerve fibres are cholinergic in nature. The knowledge of the population of cholinergic nerve fibres in mammalian AGG based upon vesicular acetylcholine transporter (VAChT) immunohistochemistry is very limited. Therefore, the aim of the present investigation was to disclose the occurrence and colocalization patterns of VAChT, dopamineâ-hydroxylase (DβH) and some neuropeptides including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and somatostatin (SOM) within nerve fibres supplying the porcine AGG. Double-immunohistochemical labellings showed that VAChT-positive nerve terminals were non-adrenergic (DâH-negative), however, many of them contained immunoreactivities to VIP, NPY and/or SOM. The coexistence patterns of these biologically active substances in nerve fibres supplying particular glands are similar but the density of cholinergic innervation varies between the organs. The innervation of the seminal vesicle and prostatic body is better developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of cholinergic nerve fibres associated with blood vessels supplying the glands contain VIP and NPY and, to a lesser degree, SOM. The possible function and origin of the cholinergic nerve fibre population are discussed.

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“…The latter was also found to contract smooth muscles of the guinea-pig prostate (Cohen and Drey, 1989). Numerous reports have indicated that VIP and NPY can directly influence the activity of the male reproductive system (Larsen et al, 1981;Stjernquist et al, 1983;Smith et al, 1984;Fahrenkrug et al, 1988;Iravani and Zar, 1994;Pinho et al, 1994;Tong et al, 1996) probably also due to their interactions (Solano et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…The ovine prostate contained VIP-IR nerve fibres in a frequency comparable to the one found in the SV and BU but the abundance of NPY-IR nerve fibres was very low. Previously, numerous VIPand NPY-containing nerve fibres were found in the human ASG (Gu et al, 1983;Adrian et al, 1984;Higgins and Gosling, 1989;Lange and Unger, 1990;Chapple et al, 1991;Tainio, 1995;Hedlund et al, 1997;Dixon et al, 2000) and in the ASG of many other animal species (Alm et al, 1977(Alm et al, , 1980Stjernquist et al, 1983;Lamano-Carvalho et al, 1986;Moss et al, 1987;Yuri, 1990;Properzi et al, 1992;Iravani and Zar, 1994;Pinho et al, 1994;Ottesen et al, 1995;Domeneghini, 1997, 1998;Chow et al, 1997;Kaleczyc et al, 1999;Czaja et al, 2000;Majewski et al, 2001;Mirabella et al, 2003). The results of the previously mentioned studies can be summarised as follows: in all species the vast majority of VIP-IR nerve fibres were localised in the mucosal layer of the SV, BU and prostate, whereas only sparse VIP-IR nerve fibres were found within the smooth muscle layer.…”

Section: Discussionmentioning

confidence: 99%

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

Arciszewski¹,

Wąsowicz

2006

Acta Veterinaria Hungarica

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Immunohistochemistry was applied to determine the distribution patterns of nerve fibres containing tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and vesicular acetylcholine transporter (VAChT) in the prostate, seminal vesicle (SV) and bulbourethral glands (BU) of male sheep. In all organs studied, cholinergic innervation was more developed than noradrenergic innervation. Numerous VAChTimmunoreactive (IR) nerve fibres were found in the muscular layer and mucosa of the SV and BU as well as in the prostate. A similar abundance of noradrenergic nerve fibres (showing immunoreactivity both to TH and DβH) was also found in both layers of the SV and BU (but not in the prostate). In the prostate a moderate density of VIP-IR nerve fibres was present but only very scarce NPY-IR nerve fibres were shown. All the studied accessory sexual glands (ASG) of male sheep contained VIP-IR nerve fibres in a similar frequency. Double immunohistochemistry revealed that the vast majority of noradrenergic nerve fibres also contained NPY. None of the noradrenergic nerve fibres showed the presence of VAChT or VIP. The possible functional significance of these findings is discussed.Key words: Autonomic innervation, neuropeptides, immunohistochemistry, accessory sexual glands, genital tract, sheep Secretions of the accessory sexual glands (ASG) contribute to the semen plasma responsible for the maintenance of a favourable environment for spermatozoa. It is generally accepted that dysfunctions of the prostate, seminal vesicles (SV) and bulbourethral glands (BU) lead to low semen quality, making the semen unsuitable for artificial insemination (AI). This is of particular importance in ruminants in which AI is routinely used. The general opinion is that the development and function of the ASG are influenced both by the endocrine and the nervous system. The application of histochemical and immunohistochemical techniques has revealed autonomic nerve fibres (noradrenergic and cholinergic)

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Immunohistochemical localization of substance P, vasoactive intestinal polypeptide and gastrin-releasing peptide in vas deferens and seminal vesicle, and the effect of these and eight other neuropeptides on resting tension and neurally evoked contractile activity

Cited by 73 publications

References 22 publications

A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation

A novel method of seminal vesicle preparation in isolated seminal vesicle experiments in the rat: ring preparation

Immunohistochemical characterisation of cholinergic nerve fibres supplying accessory genital glands in the pig

Noradrenergic and cholinergic innervation of the accessory sexual glands in male sheep

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